Abstract
With ~(45) Ca~(2+)-transport assay,the effect of red light (R) on calciumaccumulation in mitochondria isolatedfrom hypocotyl segments of 3-day-oldetiolated seedlings of mung bean(Phaseolus radiatus) was investigated.Immediately after red light irradiation,the active accumulation of Ca~(2+) in mi-tochondria was the same as that fromunirradiated segments. 10 min later,however, the uptake of calcium ion bymitochondria from R-irradiated seg-ments was lowered by 15%. More-over, since calcium accumulation inmitochondria needed energy, the activ-ities of ATPase in mitochondria wereexamined. The results demonstratedthat, 10 min after red light irradia-tion, the activities of Ca~(2+)-ATPaseand Mg~(2+)-ATPase declined by 29%and 10%, respectively. It seemed thatCa~(2+)-ATPase and part of Mg~(2+)-AT-Pase acted as calcium ion pumps regu-lating Ca~(2+) accumulation in mitochon-dria. In addition, CaM content in R-irradiated sgements was 95% higherthan that in the control, when the seg-ments were isolated 10 min after treat-ment. The activity of NAD kinase locat-ed in mitochondria was stimulated byred light when isolated 10 min after ir-radiation and the increase of enzymeactivity reached 17% only when Ca~(2+)(1 mmol/L) was present in the assaymedium. From the data reported here it issuggested that the activities of calciumpumps located in hypocotyl mitochon-dria of mung bean are depressed by redlight irradiation and the accumulationof Ca~(2+) in the mitochondria are de-creased. The concentrations of Ca~(2+)and CaM in the cytosol increased and,as a result, Ca~(2+) (and calmodulin )-de-pendent enzymes such as NAD kinasewhich play an important role in a largenumber of cellular processes are stimu-lated.
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Claims 9
Claim · model-written summary
Immediately after red light, mitochondrial active calcium accumulation was the same as in unirradiated segments.
- Taxon
- Mung Bean
.jpg?width=60)
Vigna radiatat0 - Treatment
- red light irradiatione0
- Outcome
- active accumulation of Ca~(2+)e0
- Direction
- the samee0
- Qualifier
- Immediatelye0
- Comparator
- unirradiated segmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Immediately after red light irradiation,the active accumulation of Ca~(2+) in mi-tochondria was the same as that fromunirradiated segments.”
t0“Mung Bean”
Claim · model-written summary
Ten minutes later, mitochondrial calcium uptake was lowered by 15% after red light.
- Taxon
- Mung BeanVigna radiatat0
- Treatment
- R-irradiatede0
- Outcome
- uptake of calcium ione0
- Direction
- lowerede0
- Value
- 15%e0
- Qualifier
- 10 min latere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“10 min later,however, the uptake of calcium ion bymitochondria from R-irradiated seg-ments was lowered by 15%.”
t0“Mung Bean”
Claim · model-written summary
Ca~(2+)-ATPase activity declined by 29% ten minutes after red light.
- Taxon
- Mung BeanVigna radiatat0
- Treatment
- red light irradia-tione0
- Gene
- Ca~(2+)-ATPasee0
- Outcome
- activities of Ca~(2+)-ATPasee0
- Direction
- declinede0
- Value
- 29%e0
- Qualifier
- 10 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results demonstratedthat, 10 min after red light irradia-tion, the activities of Ca~(2+)-ATPaseand Mg~(2+)-ATPase declined by 29%and 10%, respectively.”
t0“Mung Bean”
Claim · model-written summary
Mg~(2+)-ATPase activity declined by 10% ten minutes after red light.
- Taxon
- Mung BeanVigna radiatat0
- Treatment
- red light irradia-tione0
- Gene
- Mg~(2+)-ATPasee0
- Outcome
- Mg~(2+)-ATPasee0
- Direction
- declinede0
- Value
- 10%e0
- Qualifier
- 10 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results demonstratedthat, 10 min after red light irradia-tion, the activities of Ca~(2+)-ATPaseand Mg~(2+)-ATPase declined by 29%and 10%, respectively.”
t0“Mung Bean”
Claim · model-written summary
The authors suggest Ca~(2+)-ATPase and part of Mg~(2+)-ATPase act as calcium pumps regulating mitochondrial calcium accumulation.
- Taxon
- Mung BeanVigna radiatat0
- Gene
- Ca~(2+)-ATPasee0
- Outcome
- Ca~(2+) accumulatione0
- Qualifier
- calcium ion pumpse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It seemed thatCa~(2+)-ATPase and part of Mg~(2+)-AT-Pase acted as calcium ion pumps regu-lating Ca~(2+) accumulation in mitochon-dria.”
t0“Mung Bean”
Claim · model-written summary
CaM content was 95% higher than control ten minutes after red light.
- Taxon
- Mung BeanVigna radiatat0
- Treatment
- R-irradiatede0
- Gene
- CaMe0
- Outcome
- CaM contente0
- Direction
- highere0
- Value
- 95%e0
- Qualifier
- 10 mine0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition, CaM content in R-irradiated sgements was 95% higherthan that in the control, when the seg-ments were isolated 10 min after treat-ment.”
t0“Mung Bean”
Claim · model-written summary
Red light stimulated mitochondrial NAD kinase activity by 17% only with 1 mmol/L calcium in the assay, ten minutes after irradiation.
- Taxon
- Mung BeanVigna radiatat0
- Treatment
- red lighte0
- Gene
- NAD kinasee0
- Outcome
- activity of NAD kinasee0
- Direction
- stimulatede0
- Value
- 17%e0
- Qualifier
- only when Ca~(2+)(1 mmol/L) was present in the assaymediume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of NAD kinase locat-ed in mitochondria was stimulated byred light when isolated 10 min after ir-radiation and the increase of enzymeactivity reached 17% only when Ca~(2+)(1 mmol/L) was present in the assaymedium.”
t0“Mung Bean”
Claim · model-written summary
The authors suggest red light depresses hypocotyl mitochondrial calcium pumps and decreases mitochondrial calcium accumulation.
- Taxon
- mung beanVigna radiatae0
- Treatment
- redlight irradiatione0
- Outcome
- accumulationof Ca~(2+) in the mitochondriae0
- Direction
- de-creasede0
- Qualifier
- activities of calciumpumpse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From the data reported here it issuggested that the activities of calciumpumps located in hypocotyl mitochon-dria of mung bean are depressed by redlight irradiation and the accumulationof Ca~(2+) in the mitochondria are de-creased.”
Claim · model-written summary
The authors interpret increased cytosolic calcium and CaM as stimulating calcium/calmodulin-dependent enzymes such as NAD kinase.
- Taxon
- Mung BeanVigna radiatat0
- Gene
- CaMe0
- Compound
- Ca~(2+)e0
- Outcome
- enzymes such as NAD kinasee0
- Direction
- stimu-latede0
- Qualifier
- concentrations of Ca~(2+)and CaM in the cytosol increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentrations of Ca~(2+)and CaM in the cytosol increased and,as a result, Ca~(2+) (and calmodulin )-de-pendent enzymes such as NAD kinasewhich play an important role in a largenumber of cellular processes are stimu-lated.”
t0“Mung Bean”
The same questions in other papers
radiation · molecular: metabolites · Vigna radiata (mung bean)Positive and negative results
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light · molecular: metabolites · Vigna radiata (mung bean)Positive and negative results
4 papers in the same direction as this paper · 8 in the opposite direction
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9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.