Paper · 1990 · Canadian Journal of Botany

Leaf surface ultrastructure and susceptibility to ammonium nitrate injury

Iris Bitterlich; Mahesh K. Upadhyaya

12
claims
14
verbatim quotes
14
citations
4
research areas
—
Not open access

Abstract

Ammonium nitrate solution (20% N) can burn the foliage of many broadleaf weeds without significant injury to cole (broccoli, Brussels sprouts, cabbage, and cauliflower) and onion crops. Scanning electron microscopy showed that a heavy crystalline epicuticular wax layer composed of tubules, dendrites, and (or) platelets was present on the adaxial leaf surface of ammonium nitrate tolerant species. Susceptible species had little or no wax on their leaf surface. Removal of epicuticular wax by cellulose acetate stripping from tolerant cabbage leaves increased ammonium nitrate retention and salt injury. We conclude that epicuticular wax protects the foliage of tolerant species by repelling the salt solution. The solution, however, did not wet the stripped leaves evenly and leaf kill was incomplete, indicating that amorphous cuticular wax offers some protection to these leaves. The foliage of susceptible species, which lacked epicuticular wax, was damaged because it retained the solution. The presence of surface structures such as trichomes on common groundsel (Senecio vulgaris L.), lady's thumb (Polygonum persicaria L.), and shepherd's purse (Capsella bursa-pastoris (L.) Medic.) may further increase solution retention and salt injury. Key words: epicuticular wax, cole crops, onion, weed, leaf surface, trichome.

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Claims 12

Intervention effectobservedoriginal resultpositivenot significant (as stated)measured

Claim · model-written summary

Ammonium nitrate solution burned broadleaf weed foliage without significant injury to cole and onion crops.

Taxon
cole (broccoli, Brussels sprouts, cabbage, and cauliflower) and onion cropse0
Treatment
Ammonium nitrate solution (20% N)e0
Interactor
broadleaf weedse0weed
Outcome
injury to cole (broccoli, Brussels sprouts, cabbage, and cauliflower) and onion cropse0
Direction
without significant injurye0

Not reportedComparator

Verbatim

e0

“Ammonium nitrate solution (20% N) can burn the foliage of many broadleaf weeds without significant injury to cole (broccoli, Brussels sprouts, cabbage, and cauliflower) and onion crops.”

✓ located verbatim in the source · abstract, characters 0–185 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ammonium nitrate tolerant species had a heavy crystalline epicuticular wax layer on the upper leaf surface.

Taxon
ammonium nitrate tolerant speciese0
Organ / stage
adaxial leaf surfacee0
Outcome
heavy crystalline epicuticular wax layere0

Verbatim

e0

“a heavy crystalline epicuticular wax layer composed of tubules, dendrites, and (or) platelets was present on the adaxial leaf surface of ammonium nitrate tolerant species.”

✓ located verbatim in the source · abstract, characters 227–398 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Susceptible species had little or no leaf surface wax.

Taxon
Susceptible speciese0
Outcome
little or no wax on their leaf surfacee0
Direction
little or noe0

Verbatim

e0

“Susceptible species had little or no wax on their leaf surface.”

✓ located verbatim in the source · abstract, characters 399–462 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Removing epicuticular wax from cabbage leaves increased ammonium nitrate retention.

Organ / stage
leavese0
Treatment
Removal of epicuticular wax by cellulose acetate strippinge0
Outcome
ammonium nitrate retentione0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Removal of epicuticular wax by cellulose acetate stripping from tolerant cabbage leaves increased ammonium nitrate retention and salt injury.”

✓ located verbatim in the source · abstract, characters 463–604 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Removing epicuticular wax from cabbage leaves increased salt injury.

Organ / stage
leavese0
Treatment
Removal of epicuticular wax by cellulose acetate strippinge0
Outcome
salt injurye0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Removal of epicuticular wax by cellulose acetate stripping from tolerant cabbage leaves increased ammonium nitrate retention and salt injury.”

✓ located verbatim in the source · abstract, characters 463–604 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Epicuticular wax protects tolerant foliage by repelling salt solution.

Taxon
tolerant speciese0
Outcome
protects the foliagee0
Qualifier
repelling the salt solutione0

Verbatim

e0

“epicuticular wax protects the foliage of tolerant species by repelling the salt solution.”

✓ located verbatim in the source · abstract, characters 622–711 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Stripped leaves were unevenly wetted and leaf kill was incomplete.

Outcome
did not wet the stripped leaves evenly and leaf kill was incompletee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The solution, however, did not wet the stripped leaves evenly and leaf kill was incomplete”

✓ located verbatim in the source · abstract, characters 712–802 · show in text ↑
e1

“cabbage leaves”

✓ located verbatim in the source · abstract, characters 536–550 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Amorphous cuticular wax was inferred to provide some protection to stripped cabbage leaves.

Outcome
some protection to these leavese0
Qualifier
amorphous cuticular waxe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“indicating that amorphous cuticular wax offers some protection to these leaves.”

✓ located verbatim in the source · abstract, characters 804–883 · show in text ↑
e1

“cabbage leaves”

✓ located verbatim in the source · abstract, characters 536–550 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Susceptible foliage was damaged because it retained the solution.

Taxon
susceptible speciese0
Outcome
was damagede0
Qualifier
because it retained the solutione0

Verbatim

e0

“The foliage of susceptible species, which lacked epicuticular wax, was damaged because it retained the solution.”

✓ located verbatim in the source · abstract, characters 884–996 · show in text ↑
Mechanismhypothesisoriginal resultnegativemeasured

Claim · model-written summary

Surface structures may further increase solution retention and salt injury in common groundsel.

Taxon
common groundsel (Senecio vulgaris L.)Senecio vulgarise0
Outcome
solution retention and salt injurye0
Direction
increasee0
Qualifier
surface structures such as trichomese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Senecio vulgaris
Senecio vulgaris · TaxonPeter O'Connor aka anemoneprojectors from Stevenage, United Kingdom · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The presence of surface structures such as trichomes on common groundsel (Senecio vulgaris L.), lady's thumb (Polygonum persicaria L.), and shepherd's purse (Capsella bursa-pastoris (L.) Medic.) may further increase solution retention and salt injury.”

✓ located verbatim in the source · abstract, characters 997–1248 · show in text ↑
Mechanismhypothesisoriginal resultnegativemeasured

Claim · model-written summary

Surface structures may further increase solution retention and salt injury in lady's thumb.

Taxon
lady's thumb (Polygonum persicaria L.)Persicaria maculosae0
Outcome
solution retention and salt injurye0
Direction
increasee0
Qualifier
surface structures such as trichomese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persicaria maculosa
Persicaria maculosa · TaxonBjoertvedt · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The presence of surface structures such as trichomes on common groundsel (Senecio vulgaris L.), lady's thumb (Polygonum persicaria L.), and shepherd's purse (Capsella bursa-pastoris (L.) Medic.) may further increase solution retention and salt injury.”

✓ located verbatim in the source · abstract, characters 997–1248 · show in text ↑
Mechanismhypothesisoriginal resultnegativemeasured

Claim · model-written summary

Surface structures may further increase solution retention and salt injury in shepherd's purse.

Taxon
shepherd's purse (Capsella bursa-pastoris (L.) Medic.)Capsella bursa-pastorise0
Outcome
solution retention and salt injurye0
Direction
increasee0
Qualifier
surface structures such as trichomese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsella bursa-pastoris
Capsella bursa-pastoris · TaxonSaltySemanticSchmuck · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The presence of surface structures such as trichomes on common groundsel (Senecio vulgaris L.), lady's thumb (Polygonum persicaria L.), and shepherd's purse (Capsella bursa-pastoris (L.) Medic.) may further increase solution retention and salt injury.”

✓ located verbatim in the source · abstract, characters 997–1248 · show in text ↑

cellulose acetate · nutrient: content · Brassica oleracea (wild cabbage)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.