Paper · 2001 · Biologia Plantarum

Stimulation of Shoot Regeneration on Linum Hypocotyl Segments by Thidiazuron and Its Response to Light and Calcium

P. Jain; A. Rashid

5
claims
10
verbatim quotes
29
citations
2
research areas
✓
Open access

Abstract

The basal segment from hypocotyl of Linum usitatissimum L. seedling readily regenerates, to produce a large number of shoots, in a short period of 5 [ndash ] 7 d. This response was stimulated by a low concentration (0.1 [micro ]M) of thidiazuron (TDZ). TDZ was also effective in inducing regeneration in dark. A drastic reduction in regeneration response on hormone-free as well as TDZ-supplemented medium was found after inclusion of an inhibitor of calcium-uptake, lanthanum (La3+). An essentiality of calcium in the regeneration was also evidenced by an increased response with increasing concentration of calcium. At Ca2+ concentration insufficient for regeneration, inclusion of TDZ resulted in shoot formation.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thidiazuron at 0.1 µM stimulated the number of shoots regenerated from Linum hypocotyl segments.

Taxon
Linum usitatissimumLinum usitatissimume0
Treatment
thidiazuron (TDZ)e1
Outcome
number of shootse1
Direction
stimulatede1
Value
0.1 [micro ]Me1
Qualifier
a low concentratione1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Linum usitatissimum
Linum usitatissimum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The basal segment from hypocotyl of Linum usitatissimum L. seedling”

✓ located verbatim in the source · abstract, characters 0–67 · show in text ↑
e1

“The basal segment from hypocotyl of Linum usitatissimum L. seedling readily regenerates, to produce a large number of shoots, in a short period of 5 [ndash ] 7 d. This response was stimulated by a low concentration (0.1 [micro ]M) of thidiazuron (TDZ)”

✓ located verbatim in the source · abstract, characters 0–251 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thidiazuron was effective in inducing shoot regeneration in darkness.

Taxon
Linum usitatissimumLinum usitatissimume0
Treatment
TDZe2
Outcome
regeneratione2
Direction
effective in inducinge2
Setting
darke2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Linum usitatissimum
Linum usitatissimum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The basal segment from hypocotyl of Linum usitatissimum L. seedling”

✓ located verbatim in the source · abstract, characters 0–67 · show in text ↑
e2

“TDZ was also effective in inducing regeneration in dark”

✓ located verbatim in the source · abstract, characters 253–308 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Lanthanum, an inhibitor of calcium uptake, drastically reduced shoot regeneration response.

Taxon
Linum usitatissimumLinum usitatissimume0
Treatment
lanthanum (La3+)e3
Outcome
regeneration responsee3
Direction
drastic reductione3
Qualifier
hormone-free as well as TDZ-supplemented mediume3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Linum usitatissimum
Linum usitatissimum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The basal segment from hypocotyl of Linum usitatissimum L. seedling”

✓ located verbatim in the source · abstract, characters 0–67 · show in text ↑
e3

“A drastic reduction in regeneration response on hormone-free as well as TDZ-supplemented medium was found after inclusion of an inhibitor of calcium-uptake, lanthanum (La3+)”

✓ located verbatim in the source · abstract, characters 310–483 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoot regeneration response increased with increasing calcium concentration in Linum hypocotyl segments.

Taxon
Linum usitatissimumLinum usitatissimume0
Treatment
calciume4
Outcome
responsee4
Direction
increasede4
Qualifier
with increasing concentratione4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Linum usitatissimum
Linum usitatissimum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The basal segment from hypocotyl of Linum usitatissimum L. seedling”

✓ located verbatim in the source · abstract, characters 0–67 · show in text ↑
e4

“An essentiality of calcium in the regeneration was also evidenced by an increased response with increasing concentration of calcium”

✓ located verbatim in the source · abstract, characters 485–616 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At calcium concentrations insufficient for regeneration, thidiazuron resulted in shoot formation.

Taxon
Linum usitatissimumLinum usitatissimume0
Treatment
inclusion of TDZe5
Outcome
shoot formatione5
Qualifier
At Ca2+ concentration insufficient for regeneratione5

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Linum usitatissimum
Linum usitatissimum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The basal segment from hypocotyl of Linum usitatissimum L. seedling”

✓ located verbatim in the source · abstract, characters 0–67 · show in text ↑
e5

“At Ca2+ concentration insufficient for regeneration, inclusion of TDZ resulted in shoot formation”

✓ located verbatim in the source · abstract, characters 618–715 · show in text ↑

Thidiazuron · growth · Linum usitatissimum (flax)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.