Paper · 2002 · Analysis and Testing Technology and Instruments

Determination of Phenolic Glycosides from Salix Oritrepha by High-performance Liquid Chromatography

Feng Shi

3
claims
4
verbatim quotes
1
citations
2
research areas
—
Not open access

Abstract

Salicin, Salicyloylsalicin, and Leteolin 7Oglucoside were determined from S. Oritrepha. Methods: Highperformance liquid chromatography(HPLC) on a Symmetry C\-18 column (4.6 mm×250 mm, 5 μm) with acetic acidmethanol gradient elution, at column temperature of 30 ℃, flowrate of 1.1 mL/min and wavelength at 270 nm. Results: The contents of three compounds can be determined simultaneously from S. Oritrepha.The linear concentration ranges of these compounds were 527.0~105.4 μg/mL, 140.0~28.0 μg/mL, 81.0~16.2 μg/mL respectively. The average recoveries were 104.9 %, 104.6 % and 103.6 % respectively, and relative standard deviations were 2.0 %, 0.3 % and 0.3 % respectively. Conclusion: The proposed method was a simple and rapid method for simultaneous determination of the three mentioned compounds in various tissues from S. Oriterpha.

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Claims 3

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

HPLC simultaneously determined three glycosides from Salix.

Taxon
Salix OritrephaSalix oritrephat0
Outcome
contents of three compoundse0
Value
three compoundse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Salix oritrepha
Salix oritrepha · Taxon阿橋 HQ · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The contents of three compounds can be determined simultaneously from S. Oritrepha.”

✓ located verbatim in the source · abstract, characters 329–412 · show in text ↑
t0

“Salix Oritrepha”

✓ located verbatim in the source · title, characters 42–57 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The linear concentration range for salicin was 527.0~105.4 μg/mL.

Taxon
S. Oritrephae0
Outcome
linear concentration rangese0
Value
527.0~105.4 μg/mLe0

Verbatim

e0

“Salicin, Salicyloylsalicin, and Leteolin 7Oglucoside were determined from S. Oritrepha. Methods: Highperformance liquid chromatography(HPLC) on a Symmetry C\-18 column (4.6 mm×250 mm, 5 μm) with acetic acidmethanol gradient elution, at column temperature of 30 ℃, flowrate of 1.1 mL/min and wavelength at 270 nm. Results: The contents of three compounds can be determined simultaneously from S. Oritrepha.The linear concentration ranges of these compounds were 527.0~105.4 μg/mL, 140.0~28.0 μg/mL, 81.0~16.2 μg/mL respectively.”

✓ located verbatim in the source · abstract, characters 0–534 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The average recoveries for the three compounds were 104.9%, 104.6% and 103.6%, respectively.

Outcome
average recoveriese0
Value
104.9 %e0

Verbatim

e0

“The average recoveries were 104.9 %, 104.6 % and 103.6 % respectively”

✓ located verbatim in the source · abstract, characters 535–604 · show in text ↑