Abstract
Crude toxin,extracted from the culture filter of Rhizoctonia solani with different methods was different in the biological activity but had similar UV absorption spectra with the maximum absorptive wave length of about 220 nm,which indicated that they may have similar chenmical composition.The crude toxin extracted with ether had the strongest inhibition to rice radicle growth with the inhibition rate of 94.63% and could induce the characteristic symptoms of rice sheath blight.The crude toxin was continuously separated and purified by Sephadex G75 silica gel column chromatography for two times,and the active eluates were collected and called the pure toxin.On the basis of thin layer chromatography(TLC) analysis,the toxin was carbohydrate.By high performance liquid chromatography(HPLC) analysis,the toxin had four specific absorption peaks at least,which indicated that it could contain four components.Through infrared spectrum(IR) determination,the toxin could have N-H(or O-H),C=O,C-N(or C-O) signals.According to gas chromatography mass spectrometry(GCMS),the toxin contained glucose,Nacetylmannose and sucrose,but no benzoic acid,phenylacetic acid and their ramifications.
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Claims 9
Claim · model-written summary
Extraction methods yielded toxins with differing biological activity.
- Taxon
- Rhizoctonia solani
Rhizoctonia solanie0 - Outcome
- biological activitye0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Crude toxin,extracted from the culture filter of Rhizoctonia solani with different methods was different in the biological activity but had similar UV absorption spectra with the maximum absorptive wave length of about 220 nm”
Claim · model-written summary
The extracts had similar UV spectra peaking near 220 nm.
- Taxon
- Rhizoctonia solaniRhizoctonia solanie0
- Outcome
- UV absorption spectrae0
- Direction
- similare0
- Value
- about 220 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Crude toxin,extracted from the culture filter of Rhizoctonia solani with different methods was different in the biological activity but had similar UV absorption spectra with the maximum absorptive wave length of about 220 nm”
Claim · model-written summary
Ether-extracted toxin inhibited rice radicle growth by 94.63%.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- radiclee0
- Treatment
- crude toxin extracted with ethere0
- Interactor
- Rhizoctonia solaniRhizoctonia solanit0pathogen
- Outcome
- rice radicle growthe0
- Direction
- inhibitione0
- Value
- 94.63%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The crude toxin extracted with ether had the strongest inhibition to rice radicle growth with the inhibition rate of 94.63% and could induce the characteristic symptoms of rice sheath blight.”
t0“Rhizoctonia solani”
Claim · model-written summary
Ether-extracted toxin induced rice sheath blight symptoms.
- Taxon
- riceOryza sativae0
- Treatment
- crude toxin extracted with ethere0
- Interactor
- Rhizoctonia solaniRhizoctonia solanit0pathogen
- Outcome
- characteristic symptoms of rice sheath blighte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The crude toxin extracted with ether had the strongest inhibition to rice radicle growth with the inhibition rate of 94.63% and could induce the characteristic symptoms of rice sheath blight.”
t0“Rhizoctonia solani”
Claim · model-written summary
The toxin was identified as carbohydrate by TLC.
- Taxon
- Rhizoctonia solaniRhizoctonia solanit0
- Outcome
- the toxin was carbohydratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the basis of thin layer chromatography(TLC) analysis,the toxin was carbohydrate.”
t0“Rhizoctonia solani”
Claim · model-written summary
HPLC indicated the toxin could have four components.
- Taxon
- Rhizoctonia solaniRhizoctonia solanit0
- Outcome
- four specific absorption peaks at leaste0
- Value
- four specific absorption peakse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By high performance liquid chromatography(HPLC) analysis,the toxin had four specific absorption peaks at least,which indicated that it could contain four components.”
t0“Rhizoctonia solani”
Claim · model-written summary
IR indicated N-H or O-H, C=O, and C-N or C-O signals.
- Taxon
- Rhizoctonia solaniRhizoctonia solanit0
- Outcome
- N-H(or O-H),C=O,C-N(or C-O) signalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through infrared spectrum(IR) determination,the toxin could have N-H(or O-H),C=O,C-N(or C-O) signals.”
t0“Rhizoctonia solani”
Claim · model-written summary
GCMS found glucose, Nacetylmannose, and sucrose in the toxin.
- Taxon
- Rhizoctonia solaniRhizoctonia solanit0
- Outcome
- glucose,Nacetylmannose and sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“According to gas chromatography mass spectrometry(GCMS),the toxin contained glucose,Nacetylmannose and sucrose,but no benzoic acid,phenylacetic acid and their ramifications.”
t0“Rhizoctonia solani”
Claim · model-written summary
GCMS found no benzoic acid, phenylacetic acid, or derivatives in the toxin.
- Taxon
- Rhizoctonia solaniRhizoctonia solanit0
- Outcome
- no benzoic acid,phenylacetic acid and their ramificationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“According to gas chromatography mass spectrometry(GCMS),the toxin contained glucose,Nacetylmannose and sucrose,but no benzoic acid,phenylacetic acid and their ramifications.”
t0“Rhizoctonia solani”
The same questions in other papers
pathogen product · Rhizoctonia solani · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.