Abstract
One isolate(Pp3) was isolated from infected pepper in Sichuan province.The isolate was identified as Chilli veinal mottle virus(ChiVMV).The CP(coat protein) gene of the isolate was cloned by RT-PCR(reverse transcription polymerase chain reaction) and was sequenced and compared with the CP gene sequences of other ChiVMVs reported in GenBank.The results showed that the sequence of the CP gene was 861 nucleotides in length.Sequence alignment showed that this isolate shared 80.0%～88.0% identity in the CP nucleotide sequence level with those ChiVMV isolates obtained from other parts of the world.Based on the phylogenetic analysis of the CP gene of ChiVMVs,it showed that the variation of isolate Pp3 was evident.Using model plant Nicotiana tabacum the pathogenicity of this virus was studied preliminarily.The results revealed that Pp3 had higher pathagenicity and could induce systemic necrosis in N.tabacum.
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Claims 6
Claim · model-written summary
The pepper isolate Pp3 was identified as Chilli veinal mottle virus.
- Taxon
- peppere0
- Interactor
- Chilli veinal mottle virusChilli veinal mottle viruse0pathogen
- Outcome
- identified as Chilli veinal mottle virus(ChiVMV)e0
- Setting
- Sichuan provincee0
Verbatim
e0“One isolate(Pp3) was isolated from infected pepper in Sichuan province.The isolate was identified as Chilli veinal mottle virus(ChiVMV).”
Claim · model-written summary
The Pp3 coat protein gene sequence was 861 nucleotides long.
- Interactor
- Chilli veinal mottle virusChilli veinal mottle virust0pathogen
- Gene
- CP genee0
- Outcome
- sequence of the CP genee0
- Value
- 861 nucleotidese0
Not reportedTaxon
Verbatim
e0“The results showed that the sequence of the CP gene was 861 nucleotides in length.”
t0“Chilli veinal mottle virus obtained from Sichuan”
Claim · model-written summary
Pp3 shared 80.0%–88.0% coat protein nucleotide identity with other ChiVMV isolates.
- Interactor
- ChiVMVe0pathogen
- Gene
- CPe0
- Outcome
- identity in the CP nucleotide sequence levele0
- Value
- 80.0%～88.0%e0
- Comparator
- other parts of the worlde0
Not reportedTaxon
Verbatim
e0“this isolate shared 80.0%～88.0% identity in the CP nucleotide sequence level with those ChiVMV isolates obtained from other parts of the world”
Claim · model-written summary
Phylogenetic analysis showed evident variation in isolate Pp3.
Not reportedTaxon
Verbatim
e0“Based on the phylogenetic analysis of the CP gene of ChiVMVs,it showed that the variation of isolate Pp3 was evident.”
Claim · model-written summary
Pp3 had higher pathogenicity in Nicotiana tabacum.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Interactor
- Pp3e0pathogen
- Outcome
- pathagenicitye0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using model plant Nicotiana tabacum the pathogenicity of this virus was studied preliminarily.The results revealed that Pp3 had higher pathagenicity”
Claim · model-written summary
Pp3 induced systemic necrosis in Nicotiana tabacum.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Interactor
- Pp3e0pathogen
- Outcome
- systemic necrosise0
- Direction
- inducee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using model plant Nicotiana tabacum the pathogenicity of this virus was studied preliminarily.The results revealed that Pp3 had higher pathagenicity and could induce systemic necrosis in N.tabacum.”