Paper · 2001 · Shandong Agricultural Sciences

Study on nodulation and nitrogen fixation of Azospirillum brasilense induced by 2,4-D in the roots of maize

Nie Yan-fu

7
claims
14
verbatim quotes
0
citations
4
research areas
—
Not open access

Abstract

Artificial nodules on the roots were induced by treating maize seedlings with 2,4-dichlorophenoxyacetic acid (2,4-D) and Azospirillum brasilense A suitable solution of 2,4-D(0 9mg/kg)had been selected when measuring nitrogenase activity Both acetylene reduction and 15 N 2 tracer method could measure nitrogenase activity 4-hour lag period was observed when measuring nitrogenase activity by acetylene reduction method The seedlings treated with 2,4-D and Azospirillum brasilense were more tolerant to O 2 concentration than that treated with Azospirillum brasilense only The result suggested that artificial nodules induced by 2,4-D provided niche protected from O 2 for Azospirillum brasilense

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Treating maize seedlings with 2,4-D and Azospirillum induced artificial root nodules.

Taxon
maizeZea mayse0
Treatment
treating maize seedlings with 2,4-dichlorophenoxyacetic acid (2,4-D) and Azospirillum brasilensee0
Interactor
Azospirillum brasilenseAzospirillum brasilensee0symbiont
Outcome
Artificial nodules on the rootse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Artificial nodules on the roots were induced by treating maize seedlings with 2,4-dichlorophenoxyacetic acid (2,4-D) and Azospirillum brasilense”

✓ located verbatim in the source · abstract, characters 0–144 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

A 0 9mg/kg 2,4-D solution was selected for nitrogenase activity measurement.

Taxon
maizeZea mayst0
Treatment
2,4-D(0 9mg/kg)e0
Interactor
Azospirillum brasilenseAzospirillum brasilenset1symbiont
Outcome
measuring nitrogenase activitye0
Value
0 9mg/kge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“A suitable solution of 2,4-D(0 9mg/kg)had been selected when measuring nitrogenase activity”

✓ located verbatim in the source · abstract, characters 145–236 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 102–107 · show in text ↑
t1

“Azospirillum brasilense”

✓ located verbatim in the source · title, characters 45–68 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Acetylene reduction could measure nitrogenase activity.

Taxon
maizeZea mayst0
Outcome
measure nitrogenase activitye0
Comparator
15 N 2 tracer methode0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both acetylene reduction and 15 N 2 tracer method could measure nitrogenase activity”

✓ located verbatim in the source · abstract, characters 237–321 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 102–107 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The 15 N 2 tracer method could measure nitrogenase activity.

Taxon
maizeZea mayst0
Outcome
measure nitrogenase activitye0
Comparator
acetylene reductione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both acetylene reduction and 15 N 2 tracer method could measure nitrogenase activity”

✓ located verbatim in the source · abstract, characters 237–321 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 102–107 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The acetylene reduction method showed a four hour lag when measuring nitrogenase activity.

Taxon
maizeZea mayst0
Outcome
lag periode0
Value
4-houre0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“4-hour lag period was observed when measuring nitrogenase activity by acetylene reduction method”

✓ located verbatim in the source · abstract, characters 322–418 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 102–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seedlings treated with both 2,4-D and Azospirillum were more tolerant of oxygen than those receiving Azospirillum alone.

Taxon
maizeZea mayst0
Treatment
2,4-D and Azospirillum brasilensee0
Interactor
Azospirillum brasilenseAzospirillum brasilensee0symbiont
Outcome
tolerant to O 2 concentratione0
Direction
more tolerante0
Comparator
Azospirillum brasilense onlye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The seedlings treated with 2,4-D and Azospirillum brasilense were more tolerant to O 2 concentration than that treated with Azospirillum brasilense only”

✓ located verbatim in the source · abstract, characters 419–571 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 102–107 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The authors inferred that induced nodules provide Azospirillum with protection from oxygen.

Taxon
maizeZea mayst0
Treatment
2,4-De0
Interactor
Azospirillum brasilenseAzospirillum brasilensee0symbiont
Outcome
niche protected from O 2 for Azospirillum brasilensee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“artificial nodules induced by 2,4-D provided niche protected from O 2 for Azospirillum brasilense”

✓ located verbatim in the source · abstract, characters 598–695 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 102–107 · show in text ↑

2,4-D · disease · Zea mays (maize)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

Azospirillum brasilense · disease · Zea mays (maize)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.