Abstract
The improved fluorescence microscopic bioassay with cells from callus of Pinus thunbergii was introduced.Toxicity of water extracts of the callus inoculated with a pathogenic bacterial strain,GcM5(——)1A,carried by pine wood nematode,Bursaphelenchus xylophilus or inoculated with mixture of this strain and axenic pine wood nematodes was studied by the bioassay method.Results showed that the water extracts of the callus after the inoculation with the bacterial strain alone or only the axenic nematode alone did not show toxicity.Water extracts of the callus after inoculation with mixture of aseptic pine wood nematodes and the bacterial strain or the filtrated cell free fluid of the culture of the bacterial strain on NB medium showed stronger toxicity with no statistical difference among the 3 inoculation treatments.Those above results indicated that pine wood nematode promoted the production of phytotoxin by bacterium in the host tissue.Toxicity of cell free fluid filtrated of the culture of bacterial strain on NB medium,in which pine wood nematode had been added,was not significantly changed.Results also showed that pine wood nematode itself neither produced phytotoxin nor affected the toxicity of extracts from the culture of bacterium after the addition of it.
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Claims 6
Claim · model-written summary
Water extracts of pine callus inoculated with the bacterial strain alone showed no toxicity.
- Taxon
- Pinus thunbergii
Pinus thunbergiie0 - Treatment
- inoculation with the bacterial strain alonee2
- Interactor
- pathogenic bacterial straine1pathogen
- Outcome
- toxicitye2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The improved fluorescence microscopic bioassay with cells from callus of Pinus thunbergii was introduced”
e1“water extracts of the callus inoculated with a pathogenic bacterial strain,GcM5(——)1A,carried by pine wood nematode,Bursaphelenchus xylophilus”
e2“water extracts of the callus after the inoculation with the bacterial strain alone or only the axenic nematode alone did not show toxicity”
Claim · model-written summary
Water extracts of pine callus inoculated with the axenic nematode alone showed no toxicity.
- Taxon
- Pinus thunbergiiPinus thunbergiie0
- Treatment
- axenic nematode alonee2
- Interactor
- pine wood nematode
Bursaphelenchus xylophiluse1pest - Outcome
- toxicitye2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The improved fluorescence microscopic bioassay with cells from callus of Pinus thunbergii was introduced”
e1“water extracts of the callus inoculated with a pathogenic bacterial strain,GcM5(——)1A,carried by pine wood nematode,Bursaphelenchus xylophilus”
e2“water extracts of the callus after the inoculation with the bacterial strain alone or only the axenic nematode alone did not show toxicity”
Claim · model-written summary
Water extracts of pine callus inoculated with a mixture of aseptic nematodes and the bacterial strain showed stronger toxicity.
- Taxon
- Pinus thunbergiiPinus thunbergiie0
- Treatment
- inoculation with mixture of aseptic pine wood nematodes and the bacterial straine3
- Interactor
- pathogenic bacterial straine1pathogen
- Second interactor
- pine wood nematodeBursaphelenchus xylophiluse1pest
- Outcome
- toxicitye3
- Direction
- strongere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The improved fluorescence microscopic bioassay with cells from callus of Pinus thunbergii was introduced”
e1“water extracts of the callus inoculated with a pathogenic bacterial strain,GcM5(——)1A,carried by pine wood nematode,Bursaphelenchus xylophilus”
e3“Water extracts of the callus after inoculation with mixture of aseptic pine wood nematodes and the bacterial strain or the filtrated cell free fluid of the culture of the bacterial strain on NB medium showed stronger toxicity”
Claim · model-written summary
Pine wood nematode promoted the production of phytotoxin by the bacterial strain in host tissue.
- Taxon
- Pinus thunbergiiPinus thunbergiie0
- Interactor
- pathogenic bacterial straine1pathogen
- Second interactor
- pine wood nematodeBursaphelenchus xylophiluse4pest
- Outcome
- production of phytotoxine4
- Direction
- promotede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The improved fluorescence microscopic bioassay with cells from callus of Pinus thunbergii was introduced”
e1“water extracts of the callus inoculated with a pathogenic bacterial strain,GcM5(——)1A,carried by pine wood nematode,Bursaphelenchus xylophilus”
e4“pine wood nematode promoted the production of phytotoxin by bacterium in the host tissue”
Claim · model-written summary
Adding pine wood nematode to bacterial culture fluid did not significantly change its toxicity.
- Taxon
- Pinus thunbergiiPinus thunbergiie0
- Treatment
- pine wood nematode had been addede5
- Interactor
- bacterial straine5pathogen
- Second interactor
- pine wood nematodeBursaphelenchus xylophiluse5pest
- Outcome
- Toxicity of cell free fluide5
- Direction
- not significantly changede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The improved fluorescence microscopic bioassay with cells from callus of Pinus thunbergii was introduced”
e5“Toxicity of cell free fluid filtrated of the culture of bacterial strain on NB medium,in which pine wood nematode had been added,was not significantly changed”
Claim · model-written summary
Pine wood nematode itself did not produce phytotoxin.
- Taxon
- Pinus thunbergiiPinus thunbergiie0
- Interactor
- pine wood nematodeBursaphelenchus xylophiluse6pest
- Outcome
- produced phytotoxine6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The improved fluorescence microscopic bioassay with cells from callus of Pinus thunbergii was introduced”
e6“pine wood nematode itself neither produced phytotoxin nor affected the toxicity of extracts from the culture of bacterium”
The same questions in other papers
inoculation · pest · Pinus thunbergii (Japanese black pine)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.