Abstract
To this day,Agrobacterium-mediated transformation is the most widely used method in plant gene engineering,its mechanism is best understood and its effects are most satisfactory.In tissue culture for transformation of Brassica napus mediated by A.tumefaciens,vitrification of the regenerated plantlets often occurs,which normally reduces the frequency of gene transformation.The experiment reported in this paper showed that many factors affected vitrification,including phytohormone concentration,sucrose concentration,agar concentration and inoculum density.The concentration of 6-BA and ZT had significant influence on budding rate and vitrification rate.Vitrification rate increased with their concentration.6-BA 1 mg/L plus ZT 2 mg/L gave quite satisfactory results.Sucrose concentration and agar concentrations also had significant influence on vitrification rate.Vitrification and budding rate decreased with increasing sucrose and agar concentration.Sucrose 50 g/L plus agar 10 g/L was advisable.Meanwhile,inoculum density had significant influence on the occurrence of vitrification.Vitrification tended to be more serious with higher inoculum density,but budding rate was little influenced by it.A density of 20-30 inocula per dish was appropriate.
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Claims 8
Claim · model-written summary
Higher 6-BA and ZT concentration increased vitrification rate.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- 6-BA and ZTe0
- Outcome
- Vitrification ratee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“The concentration of 6-BA and ZT had significant influence on budding rate and vitrification rate.Vitrification rate increased with their concentration”
t0“Brassica napus”
Claim · model-written summary
6-BA 1 mg/L plus ZT 2 mg/L gave satisfactory results.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- 6-BA 1 mg/L plus ZT 2 mg/Le0
- Outcome
- quite satisfactory resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6-BA 1 mg/L plus ZT 2 mg/L gave quite satisfactory results”
t0“Brassica napus”
Claim · model-written summary
Increasing sucrose concentration reduced vitrification and budding rates.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- sucrosee0
- Outcome
- Vitrification and budding ratee0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitrification and budding rate decreased with increasing sucrose and agar concentration”
t0“Brassica napus”
Claim · model-written summary
Increasing agar concentration reduced vitrification and budding rates.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- agare0
- Outcome
- Vitrification and budding ratee0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitrification and budding rate decreased with increasing sucrose and agar concentration”
t0“Brassica napus”
Claim · model-written summary
The authors advised sucrose 50 g/L plus agar 10 g/L.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- Sucrose 50 g/L plus agar 10 g/Le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose 50 g/L plus agar 10 g/L was advisable”
t0“Brassica napus”
Claim · model-written summary
Higher inoculum density made vitrification more serious.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- higher inoculum densitye0
- Outcome
- Vitrificatione0
- Direction
- more seriouse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vitrification tended to be more serious with higher inoculum density”
t0“Brassica napus”
Claim · model-written summary
Inoculum density had little influence on budding rate.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- inoculum densitye0
- Outcome
- budding ratee0
- Direction
- little influencede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculum density had significant influence on the occurrence of vitrification.Vitrification tended to be more serious with higher inoculum density,but budding rate was little influenced by it”
t0“Brassica napus”
Claim · model-written summary
The authors considered 20-30 inocula per dish appropriate.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- 20-30 inocula per dishe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A density of 20-30 inocula per dish was appropriate”
t0“Brassica napus”