Abstract
Cinnamyl alcohol dehydrogenase(CAD)plays a key role in lignin biosynthesis.In this study,we report the molecular characteristics of CAD gene cloned from banana(Musa acuminata L.AAA group,cv.Cavendish)using a RACE-PCR-based strategy.MaCAD1 contained an open reading frame of 1 077 bp which encoded a polypeptide of 358 amino acids.Protein alignment showed that it contains the complete typical conserved alcohol dehydrogenase domains,which belongs to a typical CAD protein.Phylogenetic analysis showed that the deduced amino acid sequence of MaCAD1 also has high similarity to OsCAD6(CAD39907)from rice.Tissue-specific studies showed that the expression of MaCAD1 was constitutively expressed in various tissues of banana seedling.In resistant and susceptible varieties of banana after inoculation Foc TR4,the expression of MaCAD1 was decreased.While in resistant varieties the time point of MaCAD1,compared to control,increased higher than that in susceptible varieties at time points,indicated that this gene played an important role in banana resistance.MaCAD1 could be a new marker gene in banana seedling under the infection of Foc TR4.
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Claims 8
Claim · model-written summary
MaCAD1 had a 1 077 bp open reading frame encoding 358 amino acids.
Verbatim
e0“MaCAD1 contained an open reading frame of 1 077 bp which encoded a polypeptide of 358 amino acids”
t0“Banana”
Claim · model-written summary
MaCAD1 encoded a 358 amino acid polypeptide.
Verbatim
e0“MaCAD1 contained an open reading frame of 1 077 bp which encoded a polypeptide of 358 amino acids”
t0“Banana”
Claim · model-written summary
MaCAD1 contained typical conserved alcohol dehydrogenase domains.
Verbatim
e0“it contains the complete typical conserved alcohol dehydrogenase domains,which belongs to a typical CAD protein”
e1“MaCAD1 contained an open reading frame of 1 077 bp which encoded a polypeptide of 358 amino acids”
t0“Banana”
Claim · model-written summary
MaCAD1 showed high sequence similarity to rice OsCAD6.
Not reportedDirection
Verbatim
e0“the deduced amino acid sequence of MaCAD1 also has high similarity to OsCAD6(CAD39907)from rice”
t0“Banana”
Claim · model-written summary
MaCAD1 was constitutively expressed across banana seedling tissues.
Verbatim
e0“the expression of MaCAD1 was constitutively expressed in various tissues of banana seedling”
Claim · model-written summary
MaCAD1 expression decreased after Foc TR4 inoculation in resistant and susceptible banana varieties.
- Taxon
- bananae0
- Treatment
- inoculation Foc TR4e0
- Interactor
- Foc TR4
_(38100977215).jpg?width=60)
Fusarium odoratissimume0pathogen - Gene
- MaCAD1e0
- Outcome
- expression of MaCAD1e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(38100977215).jpg?width=500)
Verbatim
e0“In resistant and susceptible varieties of banana after inoculation Foc TR4,the expression of MaCAD1 was decreased”
Claim · model-written summary
At a reported time point, MaCAD1 expression increased more in resistant than susceptible banana varieties.
Verbatim
e0“in resistant varieties the time point of MaCAD1,compared to control,increased higher than that in susceptible varieties at time points”
t0“Banana”
Claim · model-written summary
The authors propose MaCAD1 as a marker gene for banana seedlings under Foc TR4 infection.
- Taxon
- bananae0
- Interactor
- Foc TR4Fusarium odoratissimume0pathogen
- Gene
- MaCAD1e0
- Outcome
- marker genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MaCAD1 could be a new marker gene in banana seedling under the infection of Foc TR4”
The same questions in other papers
inoculation · molecular: expression · MusaPositive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
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2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.