Abstract
The suspension cell apoptotic rate and mitochondria viability of two peanut varieties( 99- 1507Al- tolerant and ZH2 Al- sensitive) treated by different concentration aluminum( 0,20,100,400 μmol / L) were examined by fluorescence microscope and flow cytometry. The results showed that after Al treatment for 12h,the fluorescent light produced by FDA( Fluorescein diacetate) decreased,but increased by PI( Propidium Iodide) with the increase of Al concentration,which indicated that peanut cytoactive decreased. Content of O2 ·-and H2O2in mitochondria increased,and both mitochondrial membrane potential of two peanut varieties decreased,decrement of ZH2 was higher than 99- 1507. 50μmol / L and above Al could significantly induce peanut suspension cultures programed cell death. With the increase of Al concentration,the number of programed death cell induced by Al increased. The number was higher in ZH2 than in 99- 1507. There were significant differences between the two peanut varieties. By using flow cytometry,the apoptotic of peanut suspension cell could be inspected accurately. There was a negative correlation between cell apoptpsis rate and Al- resistance of peanut.
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Claims 9
Claim · model-written summary
Increasing Al concentration reduced peanut cell activity after 12 hours.
- Taxon
- peanut
Arachis hypogaeae0 - Treatment
- Al treatmente0
- Outcome
- peanut cytoactivee0
- Direction
- decreasede0
- Qualifier
- for 12he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after Al treatment for 12h,the fluorescent light produced by FDA( Fluorescein diacetate) decreased,but increased by PI( Propidium Iodide) with the increase of Al concentration,which indicated that peanut cytoactive decreased”
Claim · model-written summary
Mitochondrial O2 ·- content increased after Al treatment.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- Al treatmente1
- Outcome
- Content of O2 ·-e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Content of O2 ·-and H2O2in mitochondria increased,and both mitochondrial membrane potential of two peanut varieties decreased”
e1“after Al treatment for 12h,the fluorescent light produced by FDA( Fluorescein diacetate) decreased,but increased by PI( Propidium Iodide) with the increase of Al concentration,which indicated that peanut cytoactive decreased”
Claim · model-written summary
Mitochondrial H2O2 content increased after Al treatment.
- Taxon
- peanutArachis hypogaeae0
- Outcome
- H2O2in mitochondriae0
- Direction
- increasede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Content of O2 ·-and H2O2in mitochondria increased,and both mitochondrial membrane potential of two peanut varieties decreased”
Claim · model-written summary
Mitochondrial membrane potential decreased in both peanut varieties, more in ZH2.
- Taxon
- peanutArachis hypogaeae0
- Material
- ZH2e0
- Outcome
- mitochondrial membrane potentiale0
- Direction
- decreasede0
- Comparator
- 99- 1507e0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both mitochondrial membrane potential of two peanut varieties decreased,decrement of ZH2 was higher than 99- 1507”
Claim · model-written summary
Al at 50 μmol / L and above significantly induced programmed cell death in peanut cultures.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- 50μmol / L and above Ale0
- Outcome
- peanut suspension cultures programed cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“50μmol / L and above Al could significantly induce peanut suspension cultures programed cell death.”
Claim · model-written summary
The number of programmed death cells rose with Al concentration.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- Al concentratione0
- Outcome
- number of programed death celle0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With the increase of Al concentration,the number of programed death cell induced by Al increased.”
t0“peanut suspension cultures”
Claim · model-written summary
ZH2 had more programmed death cells than 99- 1507.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number was higher in ZH2 than in 99- 1507.”
t0“peanut suspension cultures”
Claim · model-written summary
Flow cytometry accurately inspected apoptosis of peanut suspension cells.
- Taxon
- peanutArachis hypogaeae0
- Outcome
- apoptotic of peanut suspension celle0
- Value
- accuratelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By using flow cytometry,the apoptotic of peanut suspension cell could be inspected accurately.”
Claim · model-written summary
Cell apoptosis rate was negatively correlated with Al resistance in peanut.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- Al- resistancee0
- Outcome
- cell apoptpsis ratee0
- Direction
- negative correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was a negative correlation between cell apoptpsis rate and Al- resistance of peanut.”
The same questions in other papers
aluminium atom · molecular: metabolites · Arachis hypogaea (peanut)Results agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.