Abstract
RNA silencing has been exploited to produce transgenic plants with resistance to viral pathogens via posttranscriptional gene silencing (PTGS). In some cases, this technology is difficult to apply due to the instability of inverted repeat (IR) constructs during cloning and plant transformation. Although such constructs have been shown to be stabilized with introns and efficiently induce RNA silencing, we found that the Pdk intron did not stabilize South African cassava mosaic virus (SACMV) silencing constructs. Therefore, we developed a method for producing long SACMV IR constructs through bisulfite-induced base pair mismatches on the sense arm prior to IR assembly. Expression of SACMV BC1 mismatched IR constructs in the model test plant Nicotiana benthamiana resulted in a reduction in viral BC1 transcript levels, hence viral replication, upon SACMV infection. Mismatched SACMV AC1 IR constructs induced PTGS more efficiently in a N. benthamiana callus system than nonmismatched IR constructs. Our novel method for IR construct generation should be applicable to many sequences where the generation of these constructs has proven difficult in the past.
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Claims 6
Claim · model-written summary
The Pdk intron did not stabilize SACMV silencing constructs.
- Taxon
- plantsViridiplantaee1
- Treatment
- Pdk introne0
- Interactor
- South African cassava mosaic virusSouth African cassava mosaic viruse0pathogen
- Outcome
- SACMV) silencing constructse0
- Direction
- did not stabilizee0
Not reportedComparatorValue
Verbatim
e0“we found that the Pdk intron did not stabilize South African cassava mosaic virus (SACMV) silencing constructs”
e1“transgenic plants with resistance to viral pathogens”
Claim · model-written summary
A bisulfite mismatch method produced long SACMV inverted-repeat constructs.
- Taxon
- plantsViridiplantaee1
- Interactor
- South African cassava mosaic virusSouth African cassava mosaic viruse2pathogen
- Outcome
- long SACMV IR constructse0
Not reportedComparatorValue
Verbatim
e0“we developed a method for producing long SACMV IR constructs through bisulfite-induced base pair mismatches on the sense arm prior to IR assembly”
e1“transgenic plants with resistance to viral pathogens”
e2“South African cassava mosaic virus (SACMV)”
Claim · model-written summary
SACMV BC1 mismatched constructs reduced viral BC1 transcript levels in infected Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- SACMV BC1 mismatched IR constructse0
- Interactor
- SACMVe0pathogen
- Gene
- viral BC1e0
- Outcome
- viral BC1 transcript levelse0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of SACMV BC1 mismatched IR constructs in the model test plant Nicotiana benthamiana resulted in a reduction in viral BC1 transcript levels, hence viral replication, upon SACMV infection.”
Claim · model-written summary
SACMV BC1 mismatched constructs reduced viral replication in infected Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- SACMV BC1 mismatched IR constructse0
- Interactor
- SACMVe0pathogen
- Outcome
- viral replicatione0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of SACMV BC1 mismatched IR constructs in the model test plant Nicotiana benthamiana resulted in a reduction in viral BC1 transcript levels, hence viral replication, upon SACMV infection.”
Claim · model-written summary
Mismatched SACMV AC1 constructs induced PTGS more efficiently than nonmismatched constructs in N. benthamiana callus.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Organ / stage
- calluse0
- Treatment
- Mismatched SACMV AC1 IR constructse0
- Interactor
- SACMVe0pathogen
- Outcome
- PTGSe0
- Direction
- more efficientlye0
- Comparator
- nonmismatched IR constructse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mismatched SACMV AC1 IR constructs induced PTGS more efficiently in a N. benthamiana callus system than nonmismatched IR constructs.”
Claim · model-written summary
The authors expect the new construct-generation method to apply to sequences for which constructs have been difficult to make.
- Taxon
- plantsViridiplantaee1
- Outcome
- IR construct generation should be applicable to many sequencese0
Verbatim
e0“Our novel method for IR construct generation should be applicable to many sequences where the generation of these constructs has proven difficult in the past.”
e1“transgenic plants with resistance to viral pathogens”
The same questions in other papers
Manihot esculenta · disease · Nicotiana benthamianaPositive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.