Abstract
Microshoots of `McIntosh' apple ( Malus domestica Borkh.) were grown on Murashige-Skoog (MS) nutrient medium supplemented with either sucrose or sorbitol or with sucrose and an elevated level of cytokinin. Shoot growth was recorded and concentrations of fructose, glucose, sorbitol, and sucrose were analyzed in nutrient media and shoots during a 6-week subculture period. Axillary branching was stimulated by high cytokinin and sorbitol media, with increased biomass production and carbohydrate use on the high-BA medium only. The sucrose in the nutrient medium was hydrolyzed to fructose and glucose, which were equally taken up by shoots. Sorbitol was taken up somewhat less effectively. The elevated level of BA decreased sucrose hydrolysis in the nutrient medium. There were high concentrations of sorbitol in shoots grown on the sorbitol medium, and sorbitol also accumulated at the end of the culture period in shoots grown on sucrose. The amount of sucrose was low, and glucose was more abundant than fructose in microshoots. The starch content of leaves was not affected by treatments or sampling time. Chemical names used: N 6 -benzyladenine (BA).
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Claims 12
Claim · model-written summary
High cytokinin stimulated apple microshoot axillary branching.
- Taxon
- Apple
Malus domesticat0 - Treatment
- high cytokinine0
- Outcome
- Axillary branchinge0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Axillary branching was stimulated by high cytokinin and sorbitol media”
t0“Apple”
Claim · model-written summary
Sorbitol medium stimulated apple microshoot axillary branching.
- Taxon
- Apple
Malus domesticat0 - Treatment
- sorbitol mediae0
- Outcome
- Axillary branchinge0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Axillary branching was stimulated by high cytokinin and sorbitol media”
t0“Apple”
Claim · model-written summary
High BA medium increased biomass production and carbohydrate use.
- Taxon
- Apple
Malus domesticat0 - Treatment
- high-BA mediume0
- Outcome
- biomass production and carbohydrate usee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“increased biomass production and carbohydrate use on the high-BA medium only”
t0“Apple”
Claim · model-written summary
Sucrose in the medium was hydrolyzed to fructose and glucose.
- Taxon
- Apple
Malus domesticat0 - Outcome
- sucrose in the nutrient medium was hydrolyzed to fructose and glucosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The sucrose in the nutrient medium was hydrolyzed to fructose and glucose”
t0“Apple”
Claim · model-written summary
Apple shoots took up fructose and glucose equally.
- Taxon
- Apple
Malus domesticat0 - Organ / stage
- shootse0
- Outcome
- fructose and glucose, which were equally taken upe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“fructose and glucose, which were equally taken up by shoots”
t0“Apple”
Claim · model-written summary
Apple shoots took up sorbitol somewhat less effectively.
- Taxon
- Apple
Malus domesticat0 - Outcome
- Sorbitol was taken upe0
- Direction
- somewhat less effectivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Sorbitol was taken up somewhat less effectively.”
t0“Apple”
Claim · model-written summary
Elevated BA decreased sucrose hydrolysis in apple nutrient medium.
- Taxon
- Apple
Malus domesticat0 - Treatment
- elevated level of BAe0
- Outcome
- sucrose hydrolysise0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The elevated level of BA decreased sucrose hydrolysis in the nutrient medium”
t0“Apple”
Claim · model-written summary
Shoots grown on sorbitol medium had high sorbitol concentrations.
- Taxon
- Apple
Malus domesticat0 - Organ / stage
- shootse0
- Treatment
- sorbitol mediume0
- Outcome
- high concentrations of sorbitole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“high concentrations of sorbitol in shoots grown on the sorbitol medium”
t0“Apple”
Claim · model-written summary
Sorbitol accumulated at the end of culture in shoots grown on sucrose.
- Taxon
- Apple
Malus domesticat0 - Organ / stage
- shootse0
- Treatment
- sucrosee0
- Outcome
- sorbitole0
- Direction
- accumulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“sorbitol also accumulated at the end of the culture period in shoots grown on sucrose”
t0“Apple”
Claim · model-written summary
Sucrose amount in apple microshoots was low.
- Taxon
- Apple
Malus domesticat0 - Outcome
- amount of sucrosee0
- Direction
- lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The amount of sucrose was low, and glucose was more abundant than fructose in microshoots”
t0“Apple”
Claim · model-written summary
Glucose was more abundant than fructose in apple microshoots.
- Taxon
- Apple
Malus domesticat0 - Outcome
- glucosee0
- Direction
- more abundante0
- Comparator
- fructosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“glucose was more abundant than fructose in microshoots”
t0“Apple”
Claim · model-written summary
Treatments and sampling time did not affect apple leaf starch content.
- Taxon
- Apple
Malus domesticat0 - Outcome
- starch content of leavese0
- Direction
- not affectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The starch content of leaves was not affected by treatments or sampling time”
t0“Apple”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.