Abstract
In this work, we found, that exogenous melatonin pretreatment improved anthocyanin accumulation (1- to 2-fold) in cabbage. To verify the relationship with melatonin and anthocyanin, an Arabidopsis mutant, snat, which expresses a defective form of the melatonin biosynthesis enzyme SNAT (Serotonin N-acetyl transferase), was employed. Under cold conditions, the foliage of wild-type Arabidopsis exhibited a deeper red color than the snat mutant. This finding further proved, that exogenous melatonin treatment was able to affect anthocyanin accumulation. To gain a better understanding of how exogenous melatonin upregulates anthocyanin, we measured gene expression in cabbage samples treated with melatonin and untreated controls. We found that the transcript levels of anthocyanin biosynthetic genes were upregulated by melatonin treatment. Moreover, melatonin treatment increased the expression levels of the transcription factors MYB, bHLH, and WD40, which constitute the transcriptional activation complex responsible for coordinative regulation of anthocyanin biosynthetic genes. We found, that free radical generation was downregulated, whereas the osmotic adjustment and antioxidant capacities were upregulated in exogenous melatonin-treated cabbage plants. We concluded, that melatonin increases anthocyanin production and benefits cabbage growth.
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Claims 7
Claim · model-written summary
Exogenous melatonin pretreatment improved anthocyanin accumulation by 1- to 2-fold in cabbage.
- Taxon
- cabbage
Brassica oleracea var. capitatae1 - Treatment
- exogenous melatonin pretreatmente1
- Outcome
- anthocyanin accumulatione1
- Direction
- improvede1
- Value
- 1- to 2-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“exogenous melatonin pretreatment improved anthocyanin accumulation (1- to 2-fold) in cabbage”
Claim · model-written summary
Under cold conditions, the foliage of wild-type Arabidopsis was deeper red than that of the snat mutant.
- Taxon
- Arabidopsis
Arabidopsise2 - Material
- snate3
- Treatment
- cold conditionse2
- Outcome
- red colore2
- Direction
- deepere2
- Comparator
- snat mutante2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Under cold conditions, the foliage of wild-type Arabidopsis exhibited a deeper red color than the snat mutant”
e3“an Arabidopsis mutant, snat, which expresses a defective form of the melatonin biosynthesis enzyme SNAT”
Claim · model-written summary
Melatonin treatment upregulated the transcript levels of anthocyanin biosynthetic genes in cabbage.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Treatment
- melatonin treatmente4
- Gene
- anthocyanin biosynthetic genese4
- Outcome
- transcript levels of anthocyanin biosynthetic genese4
- Direction
- upregulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“We found that the transcript levels of anthocyanin biosynthetic genes were upregulated by melatonin treatment”
e1“exogenous melatonin pretreatment improved anthocyanin accumulation (1- to 2-fold) in cabbage”
Claim · model-written summary
Melatonin treatment increased the expression of the transcription factors MYB, bHLH and WD40 in cabbage.
- Taxon
- cabbageBrassica oleracea var. capitatae1
- Treatment
- melatonin treatmente5
- Gene
- MYB, bHLH, and WD40e5
- Outcome
- expression levels of the transcription factorse5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“melatonin treatment increased the expression levels of the transcription factors MYB, bHLH, and WD40”
e1“exogenous melatonin pretreatment improved anthocyanin accumulation (1- to 2-fold) in cabbage”
Claim · model-written summary
Exogenous melatonin treatment downregulated free radical generation in cabbage.
- Taxon
- cabbageBrassica oleracea var. capitatae7
- Treatment
- exogenous melatonin-treatede7
- Outcome
- free radical generatione6
- Direction
- downregulatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“free radical generation was downregulated”
e7“in exogenous melatonin-treated cabbage plants”
Claim · model-written summary
Exogenous melatonin treatment upregulated osmotic adjustment and antioxidant capacities in cabbage.
- Taxon
- cabbageBrassica oleracea var. capitatae7
- Treatment
- exogenous melatonin-treatede7
- Outcome
- osmotic adjustment and antioxidant capacitiese7
- Direction
- upregulatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“the osmotic adjustment and antioxidant capacities were upregulated in exogenous melatonin-treated cabbage plants”
Claim · model-written summary
Melatonin is concluded to benefit cabbage growth.
- Taxon
- cabbageBrassica oleracea var. capitatae4
- Treatment
- melatonine4
- Outcome
- cabbage growthe4
- Direction
- benefitse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“melatonin increases anthocyanin production and benefits cabbage growth”
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