Paper · 1993 · Australian Journal of Plant Physiology

Verification of Nitrogenase Activity (C2h2 Reduction) in Azospirillum Populated, 2,4-Dichlorophenoxyacetic Acid Induced, Root Structures of Wheat

D Yu; IR Kennedy; YT Tchan

6
claims
11
verbatim quotes
23
citations
2
research areas
—
Not open access

Abstract

The characteristics of C2H4 formation and H2 evolution associated with 2,4-D induced root structures on wheat seedlings inoculated with Azospirillum brasilense Sp7 have been investigated. C2H4 formation and H2 evolution with 2,4-D treatment is several times greater than that by wheat seedlings inoculated with Azospirillum alone, as the result of the formation of a niche for azospirilla protected from O2. This increased C2H4 production in 2,4-D treated seedlings was proportional to C2H2 concentration and it was strongly inhibited by nitrite and ammonia. The inhibition increased with greater ammonia and nitrite concentration, but was reversible. The increased rate of C2H2 reduction activity was correlated with 15N enrichment in 2,4-D treated seedlings. These results confirm that the C2H4 formation was C2H2 dependent and represented the nitrogenase activity of A. brasilense associated with the 2,4-D induced root structures of wheat seedlings rather than C2H2 produced by plant tissues.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

2,4-D increased C2H4 formation and H2 evolution severalfold versus Azospirillum inoculation alone.

Taxon
wheat seedlingsTriticum aestivume0
Treatment
2,4-D treatmente0
Interactor
AzospirillumAzospirillume0symbiont
Outcome
C2H4 formation and H2 evolutione0
Direction
greatere0
Value
several timese0
Comparator
wheat seedlings inoculated with Azospirillum alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Azospirillum
Azospirillum · InteractorFadelblaid · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“C2H4 formation and H2 evolution with 2,4-D treatment is several times greater than that by wheat seedlings inoculated with Azospirillum alone”

✓ located verbatim in the source · abstract, characters 188–329 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Increased C2H4 production was proportional to C2H2 concentration.

Treatment
2,4-De0
Compound
C2H2e0
Outcome
C2H4 productione0
Direction
increasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This increased C2H4 production in 2,4-D treated seedlings was proportional to C2H2 concentration”

✓ located verbatim in the source · abstract, characters 408–504 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 140–145 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Nitrite and ammonia strongly inhibited C2H4 production.

Treatment
nitrite and ammoniae0
Outcome
C2H4 productione1
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“it was strongly inhibited by nitrite and ammonia.”

✓ located verbatim in the source · abstract, characters 509–558 · show in text ↑
e1

“This increased C2H4 production in 2,4-D treated seedlings was proportional to C2H2 concentration”

✓ located verbatim in the source · abstract, characters 408–504 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 140–145 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Inhibition increased with greater ammonia and nitrite concentrations and was reversible.

Compound
ammonia and nitritee0
Outcome
inhibitione0
Direction
increasede0
Qualifier
reversiblee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The inhibition increased with greater ammonia and nitrite concentration, but was reversible.”

✓ located verbatim in the source · abstract, characters 559–651 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 140–145 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

C2H2 reduction activity correlated with 15N enrichment in treated seedlings.

Treatment
2,4-De0
Compound
15Ne0
Outcome
C2H2 reduction activitye0
Direction
increasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The increased rate of C2H2 reduction activity was correlated with 15N enrichment in 2,4-D treated seedlings.”

✓ located verbatim in the source · abstract, characters 652–760 · show in text ↑
t0

“Wheat”

✓ located verbatim in the source · title, characters 140–145 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors attributed C2H4 formation to Azospirillum nitrogenase associated with induced wheat root structures.

Taxon
wheat seedlingsTriticum aestivume0
Organ / stage
root structurese0
Interactor
A. brasilenseAzospirillum brasilensee0symbiont
Outcome
C2H4 formatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“the C2H4 formation was C2H2 dependent and represented the nitrogenase activity of A. brasilense associated with the 2,4-D induced root structures of wheat seedlings”

✓ located verbatim in the source · abstract, characters 788–952 · show in text ↑

ammonia · yield · Triticum aestivum (wheat)Positive and negative results

21 papers: 15 positive, 6 negative or no effect

5 papers in the same direction as this paper · 15 in the opposite direction

  1. positive
  2. positive
  3. positive

17 papers more in this group

See all 21 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.