Abstract
Phenylalanine ammonia-lyase (PAL) was purified from bamboo shoots (Bambusa oldhami Munro) through steps of DEAE-Sephacel column chromatography, Sepharose CL-6B gel filtration, and a second DEAE-Sephacel column chromatography. Polyacrylamide gel electrophoresis showed that the purified enzyme (Mr 360, 000) still contained two minor bands with much slower mobilities (Mr ?? 660, 000) than the major one. The minor band could be the aggregated forms of native PAL, based on the fact that only a single band (Mr 86, 000) was observed upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectric focusing, and all bands shared common antigenic determinants as revealed by immunoblotting done with an antiserum raised against the major band. There was a sharp rise of PAL activity of bamboo shoots after harvest, and this was only partly due to the de novo synthesis of this enzyme as measured by Laurell's rocket immunoelectrophoresis.
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Claims 5
Claim · model-written summary
Purified bamboo shoot PAL had a major electrophoretic band and two slower minor bands.
Verbatim
e0“Phenylalanine ammonia-lyase (PAL) was purified from bamboo shoots (Bambusa oldhami Munro)”
e1“the purified enzyme (Mr 360, 000) still contained two minor bands with much slower mobilities (Mr ?? 660, 000) than the major one”
Claim · model-written summary
A single 86,000 Mr band appeared under SDS electrophoresis or isoelectric focusing, supporting aggregated minor forms.
Verbatim
e0“bamboo shoots (Bambusa oldhami Munro)”
e1“only a single band (Mr 86, 000) was observed upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectric focusing”
Claim · model-written summary
All PAL bands shared antigenic determinants.
Verbatim
e0“bamboo shoots (Bambusa oldhami Munro)”
e1“all bands shared common antigenic determinants as revealed by immunoblotting”
Claim · model-written summary
PAL activity rose sharply in bamboo shoots after harvest.
Verbatim
e0“There was a sharp rise of PAL activity of bamboo shoots after harvest”
Claim · model-written summary
De novo PAL synthesis accounted for only part of the postharvest activity increase.
Verbatim
e0“There was a sharp rise of PAL activity of bamboo shoots after harvest, and this was only partly due to the de novo synthesis of this enzyme”