Paper · 2014 · HortScience

An In Vitro Germination Technique for Some Stone Fruit Species: the Embryo Isolated from Cotyledons Successfully Germinated without Cold Pre-treatment of Seeds

Bekir Şan; Adnan Nurhan Yildirim; Fatma Yildirim

18
claims
29
verbatim quotes
22
citations
3
research areas
✓
Open access

Abstract

Seeds of temperate fruit species need a long time to germinate as a result of their requirement of stratification or cold treatment. Therefore, fast and uniform germination techniques are desirable and important for fruit tree propagation and especially for breeding studies. The effects of combinations of benzylaminopurine (BAP) and gibberellic acid (GA 3 ) on in vitro embryo germination of apricot, peach, and wild cherry were determined without seed cold pretreatment. The results showed that no germination was recorded in all the seeds with testa. In the seeds without testa, no germination (wild cherry) or limited germination (less than 10%) was recorded. When the embryos separated from cotyledons were cultured, successful germination was obtained for all species. In general, the addition of different combinations of BAP and GA 3 into the Murashige and Skoog (MS) medium significantly increased the germination ratios of embryos without cotyledons in all species. For wild cherry, the best treatment (66.7% germination) was MS media containing 0.5 mg·L −1 BAP + 2.0 mg·L −1 GA 3 or 0.5 mg·L −1 BAP + 4.0 mg·L −1 GA 3 . For peach, the best treatment (86.7% germination) was MS medium supplemented with 0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3 . For apricot, the best treatment (93.3%) was MS media containing 0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3 , 1.0 mg·L −1 BAP + 1.0 mg·L −1 GA 3 , or 1.0 mg·L −1 BAP + 2.0 mg·L −1 GA 3 .

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Claims 18

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Seeds with testa did not germinate in apricot.

Taxon
apricotPrunus armeniacae1
Organ / stage
seeds with testae0
Outcome
germinatione0
Direction
no germinatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus armeniaca
Prunus armeniaca · TaxonFir0002 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“no germination was recorded in all the seeds with testa”

✓ located verbatim in the source · abstract, characters 498–553 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Seeds with testa did not germinate in peach.

Organ / stage
seeds with testae0
Outcome
germinatione0
Direction
no germinatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“no germination was recorded in all the seeds with testa”

✓ located verbatim in the source · abstract, characters 498–553 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Seeds with testa did not germinate in wild cherry.

Taxon
wild cherrye1
Organ / stage
seeds with testae0
Outcome
germinatione0
Direction
no germinatione0

Verbatim

e0

“no germination was recorded in all the seeds with testa”

✓ located verbatim in the source · abstract, characters 498–553 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Wild cherry seeds without testa did not germinate.

Taxon
wild cherrye0
Organ / stage
seeds without testae0
Outcome
germinatione0
Direction
no germinatione0

Verbatim

e0

“In the seeds without testa, no germination (wild cherry) or limited germination (less than 10%) was recorded”

✓ located verbatim in the source · abstract, characters 555–663 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Other seeds without testa had limited germination below 10%.

Taxon
apricotPrunus armeniacae1
Organ / stage
seeds without testae0
Outcome
germinatione0
Direction
limited germinatione0
Value
less than 10%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus armeniaca
Prunus armeniaca · TaxonFir0002 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In the seeds without testa, no germination (wild cherry) or limited germination (less than 10%) was recorded”

✓ located verbatim in the source · abstract, characters 555–663 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Peach seeds without testa had limited germination below 10%.

Organ / stage
seeds without testae0
Outcome
germinatione0
Direction
limited germinatione0
Value
less than 10%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“In the seeds without testa, no germination (wild cherry) or limited germination (less than 10%) was recorded”

✓ located verbatim in the source · abstract, characters 555–663 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Embryos separated from cotyledons germinated successfully in apricot.

Taxon
apricotPrunus armeniacae1
Organ / stage
embryos separated from cotyledonse0
Outcome
successful germinatione0
Value
all speciese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus armeniaca
Prunus armeniaca · TaxonFir0002 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“When the embryos separated from cotyledons were cultured, successful germination was obtained for all species”

✓ located verbatim in the source · abstract, characters 665–774 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Embryos separated from cotyledons germinated successfully in peach.

Organ / stage
embryos separated from cotyledonse0
Outcome
successful germinatione0
Value
all speciese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“When the embryos separated from cotyledons were cultured, successful germination was obtained for all species”

✓ located verbatim in the source · abstract, characters 665–774 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Embryos separated from cotyledons germinated successfully in wild cherry.

Taxon
wild cherrye1
Organ / stage
embryos separated from cotyledonse0
Outcome
successful germinatione0
Value
all speciese0

Verbatim

e0

“When the embryos separated from cotyledons were cultured, successful germination was obtained for all species”

✓ located verbatim in the source · abstract, characters 665–774 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BAP and GA 3 combinations significantly increased embryo germination in apricot.

Taxon
apricotPrunus armeniacae1
Treatment
different combinations of BAP and GA 3e0
Outcome
germination ratios of embryos without cotyledonse0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus armeniaca
Prunus armeniaca · TaxonFir0002 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the addition of different combinations of BAP and GA 3 into the Murashige and Skoog (MS) medium significantly increased the germination ratios of embryos without cotyledons in all species”

✓ located verbatim in the source · abstract, characters 788–975 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BAP and GA 3 combinations significantly increased embryo germination in peach.

Treatment
different combinations of BAP and GA 3e0
Outcome
germination ratios of embryos without cotyledonse0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“the addition of different combinations of BAP and GA 3 into the Murashige and Skoog (MS) medium significantly increased the germination ratios of embryos without cotyledons in all species”

✓ located verbatim in the source · abstract, characters 788–975 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BAP and GA 3 combinations significantly increased embryo germination in wild cherry.

Taxon
wild cherrye1
Treatment
different combinations of BAP and GA 3e0
Outcome
germination ratios of embryos without cotyledonse0
Direction
increasede0

Not reportedComparator

Verbatim

e0

“the addition of different combinations of BAP and GA 3 into the Murashige and Skoog (MS) medium significantly increased the germination ratios of embryos without cotyledons in all species”

✓ located verbatim in the source · abstract, characters 788–975 · show in text ↑
e1

“apricot, peach, and wild cherry”

✓ located verbatim in the source · abstract, characters 394–425 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Wild cherry germination reached 66.7% with MS medium containing 0.5 mg·L −1 BAP and 2.0 mg·L −1 GA 3.

Taxon
wild cherrye0
Treatment
0.5 mg·L −1 BAP + 2.0 mg·L −1 GA 3e0
Outcome
germinatione0
Direction
beste0
Value
66.7%e0

Verbatim

e0

“For wild cherry, the best treatment (66.7% germination) was MS media containing 0.5 mg·L −1 BAP + 2.0 mg·L −1 GA 3 or 0.5 mg·L −1 BAP + 4.0 mg·L −1 GA 3”

✓ located verbatim in the source · abstract, characters 977–1129 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Wild cherry germination reached 66.7% with MS medium containing 0.5 mg·L −1 BAP and 4.0 mg·L −1 GA 3.

Taxon
wild cherrye0
Treatment
0.5 mg·L −1 BAP + 4.0 mg·L −1 GA 3e0
Outcome
germinatione0
Direction
beste0
Value
66.7%e0

Verbatim

e0

“For wild cherry, the best treatment (66.7% germination) was MS media containing 0.5 mg·L −1 BAP + 2.0 mg·L −1 GA 3 or 0.5 mg·L −1 BAP + 4.0 mg·L −1 GA 3”

✓ located verbatim in the source · abstract, characters 977–1129 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Peach germination reached 86.7% with 0.5 mg·L −1 BAP and 3.0 mg·L −1 GA 3.

Treatment
0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3e0
Outcome
germinatione0
Direction
beste0
Value
86.7%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“For peach, the best treatment (86.7% germination) was MS medium supplemented with 0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3”

✓ located verbatim in the source · abstract, characters 1132–1248 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Apricot germination reached 93.3% with 0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3.

Taxon
apricotPrunus armeniacae0
Treatment
0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3e0
Outcome
best treatmente0
Direction
beste0
Value
93.3%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus armeniaca
Prunus armeniaca · TaxonFir0002 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“For apricot, the best treatment (93.3%) was MS media containing 0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3 , 1.0 mg·L −1 BAP + 1.0 mg·L −1 GA 3 , or 1.0 mg·L −1 BAP + 2.0 mg·L −1 GA 3”

✓ located verbatim in the source · abstract, characters 1251–1426 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Apricot germination reached 93.3% with 1.0 mg·L −1 BAP + 1.0 mg·L −1 GA 3.

Taxon
apricotPrunus armeniacae0
Treatment
1.0 mg·L −1 BAP + 1.0 mg·L −1 GA 3e0
Outcome
best treatmente0
Direction
beste0
Value
93.3%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus armeniaca
Prunus armeniaca · TaxonFir0002 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“For apricot, the best treatment (93.3%) was MS media containing 0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3 , 1.0 mg·L −1 BAP + 1.0 mg·L −1 GA 3 , or 1.0 mg·L −1 BAP + 2.0 mg·L −1 GA 3”

✓ located verbatim in the source · abstract, characters 1251–1426 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Apricot germination reached 93.3% with 1.0 mg·L −1 BAP + 2.0 mg·L −1 GA 3.

Taxon
apricotPrunus armeniacae0
Treatment
1.0 mg·L −1 BAP + 2.0 mg·L −1 GA 3e0
Outcome
best treatmente0
Direction
beste0
Value
93.3%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus armeniaca
Prunus armeniaca · TaxonFir0002 · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“For apricot, the best treatment (93.3%) was MS media containing 0.5 mg·L −1 BAP + 3.0 mg·L −1 GA 3 , 1.0 mg·L −1 BAP + 1.0 mg·L −1 GA 3 , or 1.0 mg·L −1 BAP + 2.0 mg·L −1 GA 3”

✓ located verbatim in the source · abstract, characters 1251–1426 · show in text ↑

Gibberellic acid · growth · Prunus persica (peach)Results differ

23 papers: 19 positive, 4 negative or no effect

18 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. negative
  3. negative

19 papers more in this group

See all 23 papers →

N-benzyladenine · growth · Prunus persica (peach)Results differ

24 papers: 20 positive, 4 negative or no effect

19 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. no effect

20 papers more in this group

See all 24 papers →

N-benzyladenine · growth · Prunus armeniaca (apricot)Positive and negative results

11 papers: 9 positive, 2 negative or no effect

8 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

7 papers more in this group

See all 11 papers →

Gibberellic acid · growth · Prunus armeniaca (apricot)Positive and negative results

12 papers: 10 positive, 2 negative or no effect

9 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

8 papers more in this group

See all 12 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.