Abstract
The concentration of non-enzymatic antioxidants such as ascorbate and glutathione in tissues is one of the major plant responses to biotic and many abiotic stresses, including metals. Therefore, it is crucial to develop the most effective methods for simultaneous quantitative analysis of these antioxidants. Capillary zone electrophoresis allows relatively fast and effective analysis. The aim of the paper was to apply and optimise the capillary electrophoresis conditions for simultaneous determination of glutathione, glutathione disulphide, ascorbate, and dehydroascorbate in small plant tissue samples exposed to copper and cadmium. The method ensures good linearity and reproducibility, with correlation coefficients 0.988 for ascorbate and 0.999 for glutathione and glutathione disulphide, and with detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively. Cu stress was found to increase the ascorbate concentration and glutathione content in leaves, while Cd increased glutathione in the oldest leaf segments and root.
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Claims 8
Claim · model-written summary
Ascorbate measurement had correlation coefficient 0.988.
Zea maysNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“correlation coefficients 0.988 for ascorbate”
t0“Zea mays”
Claim · model-written summary
Glutathione and glutathione disulphide measurement had correlation coefficient 0.999.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.999 for glutathione and glutathione disulphide”
t0“Zea mays”
Claim · model-written summary
Ascorbate detection limit was approximately 2.50 ppm.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively”
t0“Zea mays”
Claim · model-written summary
Glutathione detection limit was approximately 0.65 ppm.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively”
t0“Zea mays”
Claim · model-written summary
Glutathione disulphide detection limit was approximately 0.50 ppm.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“detection limits approximately 2.50, 0.65 and 0.50 ppm, respectively”
t0“Zea mays”
Claim · model-written summary
Copper stress increased leaf ascorbate concentration and glutathione content.
- Organ / stage
- leavese0
- Treatment
- Cu stresse0
- Outcome
- ascorbate concentration and glutathione contente0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cu stress was found to increase the ascorbate concentration and glutathione content in leaves”
t0“Zea mays”
Claim · model-written summary
Cadmium increased glutathione in the oldest leaf segments.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cd increased glutathione in the oldest leaf segments and root”
t0“Zea mays”
Claim · model-written summary
Cadmium increased root glutathione.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cd increased glutathione in the oldest leaf segments and root”
t0“Zea mays”
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.