Abstract
Phytic acid (PA) is a major source of inorganic phosphate (Pi) in the soil; however, the plant lacks the capacity to utilize it for Pi nutrition and growth. Microbial phytases constitute a group of enzymes that are able to remobilize Pi from PA. Thus, the use of these phytases to increase the capacity of higher plants to remobilize Pi from PA is of agronomical interest. In the current study, we generate transgenic Arabidopsis lines (ePHY) overexpressing an extracellular form of the phytase PHY-US417 of Bacillus subtilis, which are characterized by high levels of secreted phytase activity. In the presence of PA as sole source of Pi, while the wild-type plants show hallmark of Pi deficiency phenotypes, including the induction of the expression of Pi starvation-induced genes (PSI, e.g. PHT1;4) and the inhibition of growth capacity, the ePHY overexpressing lines show a higher biomass production and no PSI induction. Interestingly, when co-cultured with ePHY overexpressors, wild-type Arabidopsis plants (or tobacco) show repression of the PSI genes, improvement of Pi content and increases in biomass production. In line with these results, mutants in the high-affinity Pi transporters, namely pht1;1 and pht1;1-1;4, both fail to accumulate Pi and to grow when co-cultured with ePHY overexpressors. Taken together, these data demonstrate the potential of secreted phytases in improving the Pi content and enhancing growth of not only the transgenic lines but also the neighbouring plants.
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Claims 8
Claim · model-written summary
ePHY lines had high secreted phytase activity.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- ePHYe0
- Gene
- PHY-US417e0
- Outcome
- secreted phytase activitye0
- Direction
- high levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ePHY) overexpressing an extracellular form of the phytase PHY-US417 of Bacillus subtilis, which are characterized by high levels of secreted phytase activity”
t0“Arabidopsis”
Claim · model-written summary
With phytic acid as sole phosphate source, wild type plants showed phosphate deficiency phenotypes and inhibited growth.
- Taxon
- ArabidopsisArabidopsist0
- Material
- wild-type plantse0
- Treatment
- PA as sole source of Pie0
- Gene
- PHT1;4e0
- Outcome
- Pi deficiency phenotypese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of PA as sole source of Pi, while the wild-type plants show hallmark of Pi deficiency phenotypes, including the induction of the expression of Pi starvation-induced genes (PSI, e.g. PHT1;4) and the inhibition of growth capacity”
t0“Arabidopsis”
Claim · model-written summary
ePHY lines on phytic acid showed higher biomass production.
- Taxon
- ArabidopsisArabidopsist0
- Material
- ePHYe0
- Outcome
- biomass productione0
- Direction
- highere0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ePHY overexpressing lines show a higher biomass production and no PSI induction”
t0“Arabidopsis”
Claim · model-written summary
ePHY lines showed no induction of phosphate starvation genes.
- Taxon
- ArabidopsisArabidopsist0
- Material
- ePHYe0
- Outcome
- PSI inductione0
- Direction
- no PSI inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ePHY overexpressing lines show a higher biomass production and no PSI induction”
t0“Arabidopsis”
Claim · model-written summary
Wild type Arabidopsis co-cultured with ePHY plants showed repression of PSI genes.
- Taxon
- ArabidopsisArabidopsise0
- Material
- wild-type Arabidopsise0
- Treatment
- co-cultured with ePHY overexpressorse0
- Outcome
- PSI genese0
- Direction
- repressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when co-cultured with ePHY overexpressors, wild-type Arabidopsis plants (or tobacco) show repression of the PSI genes, improvement of Pi content and increases in biomass production”
Claim · model-written summary
Wild type Arabidopsis co-cultured with ePHY plants improved phosphate content and biomass.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- co-cultured with ePHY overexpressorse0
- Outcome
- Pi content and increases in biomass productione0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when co-cultured with ePHY overexpressors, wild-type Arabidopsis plants (or tobacco) show repression of the PSI genes, improvement of Pi content and increases in biomass production”
Claim · model-written summary
Tobacco co-cultured with ePHY plants showed repression of PSI genes and improved Pi content and biomass.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- co-cultured with ePHY overexpressorse0
- Outcome
- PSI genes, improvement of Pi content and increases in biomass productione0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when co-cultured with ePHY overexpressors, wild-type Arabidopsis plants (or tobacco) show repression of the PSI genes, improvement of Pi content and increases in biomass production”
Claim · model-written summary
Phosphate transporter mutants failed to accumulate Pi or grow with ePHY neighbors.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pht1;1 and pht1;1-1;4e0
- Treatment
- co-cultured with ePHY overexpressorse0
- Gene
- pht1;1 and pht1;1-1;4e0
- Outcome
- accumulate Pi and to growe0
- Direction
- faile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutants in the high-affinity Pi transporters, namely pht1;1 and pht1;1-1;4, both fail to accumulate Pi and to grow when co-cultured with ePHY overexpressors”
t0“Arabidopsis”
The same questions in other papers
overexpression · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
15 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- negative
- negative
18 papers more in this group
See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.