Abstract
An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks. Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%). Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival. In addition, ultrastructural studies showed that rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens, possibly due to the presence of wide spaces. Meanwhile, the leaves of in vivo specimens had more open stomata compared to in vitro specimens, yet their paracytic stomata structures were similar. Hence, there were no abnormalities or major differences between in vitro regenerants and mother plants.
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Claims 8
Claim · model-written summary
MS medium with 3.0 mg/L BAP and 1.5 mg/L NAA produced the highest shoots, 73.3 ± 0.5%.
- Taxon
- plante0
- Treatment
- MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAAe0
- Outcome
- number of shootse0
- Direction
- higheste0
- Value
- 73.3 ± 0.5%e0
Not reportedComparator
Verbatim
e0“Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%)”
Claim · model-written summary
The same medium produced the highest roots, 86.7 ± 0.4%.
- Taxon
- Canna indica
.jpg?width=60)
Canna indicat0 - Treatment
- 3.0 mg/L BAP plus 1.5 mg/L NAAe1
- Outcome
- rootse0
- Value
- 86.7 ± 0.4%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Canna indica”
e0“roots (86.7 ± 0.4%) after 2 weeks”
e1“MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA”
Claim · model-written summary
MS enriched with 7.0 mg/L BAP was optimal for multiple shoot regeneration at 33.0 ± 0.5%.
- Taxon
- Canna indicaCanna indicat0
- Treatment
- MS enriched with 7.0 mg/L BAPe0
- Outcome
- multiple shoots regeneratione0
- Direction
- optimume0
- Value
- 33.0 ± 0.5%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna indica”
e0“optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%)”
Claim · model-written summary
Greenhouse acclimatization yielded 75% survival.
- Taxon
- Canna indicaCanna indicat0
- Outcome
- survivale0
- Value
- 75%e0
- Setting
- greenhousee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna indica”
e0“acclimatized in the greenhouse, with 75% survival”
Claim · model-written summary
In vitro rhizomes were underdeveloped relative to in vivo specimens.
- Taxon
- Canna indicaCanna indicat0
- Outcome
- rhizomese0
- Direction
- underdevelopede0
- Comparator
- in vivo specimense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna indica”
e0“rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens”
Claim · model-written summary
In vivo leaves had more open stomata than in vitro leaves.
- Taxon
- Canna indicaCanna indicat0
- Organ / stage
- leavese0
- Outcome
- open stomatae0
- Direction
- moree0
- Comparator
- in vitro specimense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna indica”
e0“the leaves of in vivo specimens had more open stomata compared to in vitro specimens”
Claim · model-written summary
Paracytic stomatal structures were similar between in vivo and in vitro specimens.
- Taxon
- Canna indicaCanna indicat0
- Outcome
- paracytic stomata structurese0
- Direction
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna indica”
e0“yet their paracytic stomata structures were similar”
Claim · model-written summary
Authors found no major differences between regenerants and mother plants.
- Taxon
- Canna indicaCanna indicat0
- Outcome
- abnormalities or major differencese0
- Direction
- noe0
- Comparator
- mother plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Canna indica”
e0“there were no abnormalities or major differences between in vitro regenerants and mother plants”