Abstract
Because copper nanoparticles are being increasingly used in agriculture as pesticides, it is important to assess their potential implications for agriculture. Concerns have been raised about the bioaccumulation of nano-Cu and their toxicity to crop plants. Here, the response of cucumber plants in hydroponic culture at early development stages to two concentrations of nano-Cu (10 and 20 mg/L) was evaluated by proton nuclear magnetic resonance spectroscopy ((1)H NMR) and gas chromatography-mass spectrometry (GC-MS) based metabolomics. Changes in mineral nutrient metabolism induced by nano-Cu were determined by inductively coupled plasma-mass spectrometry (ICP-MS). Results showed that nano-Cu at both concentrations interferes with the uptake of a number of micro- and macro-nutrients, such as Na, P, S, Mo, Zn, and Fe. Metabolomics data revealed that nano-Cu at both levels triggered significant metabolic changes in cucumber leaves and root exudates. The root exudate metabolic changes revealed an active defense mechanism against nano-Cu stress: up-regulation of amino acids to sequester/exclude Cu/nano-Cu; down-regulation of citric acid to reduce the mobilization of Cu ions; ascorbic acid up-regulation to combat reactive oxygen species; and up-regulation of phenolic compounds to improve antioxidant system. Thus, we demonstrate that nontargeted (1)H NMR and GC-MS based metabolomics can successfully identify physiological responses induced by nanoparticles. Root exudates metabolomics revealed important detoxification mechanisms.
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Claims 8
Claim · model-written summary
Nano-Cu at both concentrations interfered with uptake of several nutrients.
- Taxon
- cucumber
Cucumis sativust0 - Treatment
- nano-Cu at both concentrationse0
- Outcome
- uptake of a number of micro- and macro-nutrients, such as Na, P, S, Mo, Zn, and Fee0
- Direction
- interferese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results showed that nano-Cu at both concentrations interferes with the uptake of a number of micro- and macro-nutrients, such as Na, P, S, Mo, Zn, and Fe.”
t0“Cucumber Plant”
Claim · model-written summary
Nano-Cu caused significant metabolic changes in cucumber leaves.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- leavese0
- Treatment
- nano-Cu at both levelse0
- Outcome
- metabolic changes in cucumber leavese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Metabolomics data revealed that nano-Cu at both levels triggered significant metabolic changes in cucumber leaves and root exudates.”
Claim · model-written summary
Nano-Cu caused significant metabolic changes in cucumber root exudates.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- root exudatese0
- Treatment
- nano-Cu at both levelse0
- Outcome
- metabolic changes in cucumber leaves and root exudatese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Metabolomics data revealed that nano-Cu at both levels triggered significant metabolic changes in cucumber leaves and root exudates.”
Claim · model-written summary
Amino acids were up-regulated in root exudates under nano-Cu stress to sequester or exclude Cu.
- Taxon
- cucumberCucumis sativust0
- Treatment
- nano-Cue0
- Outcome
- up-regulation of amino acidse0
- Qualifier
- sequester/exclude Cu/nano-Cue0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“up-regulation of amino acids to sequester/exclude Cu/nano-Cu”
t0“Cucumber Plant”
Claim · model-written summary
Citric acid was down-regulated, which the authors linked to reduced Cu-ion mobilization.
- Taxon
- cucumberCucumis sativust0
- Compound
- Cu ionse0
- Outcome
- down-regulation of citric acide0
- Qualifier
- reduce the mobilization of Cu ionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down-regulation of citric acid to reduce the mobilization of Cu ions”
t0“Cucumber Plant”
Claim · model-written summary
Ascorbic acid was up-regulated to combat reactive oxygen species.
- Taxon
- cucumberCucumis sativust0
- Outcome
- ascorbic acid up-regulatione0
- Qualifier
- combat reactive oxygen speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ascorbic acid up-regulation to combat reactive oxygen species”
t0“Cucumber Plant”
Claim · model-written summary
Phenolic compounds were up-regulated to improve the antioxidant system.
- Taxon
- cucumberCucumis sativust0
- Outcome
- up-regulation of phenolic compoundse0
- Qualifier
- improve antioxidant systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“up-regulation of phenolic compounds to improve antioxidant system”
t0“Cucumber Plant”
Claim · model-written summary
Untargeted NMR and GC-MS metabolomics identified physiological responses induced by nanoparticles.
- Taxon
- cucumberCucumis sativust0
- Outcome
- physiological responses induced by nanoparticlese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we demonstrate that nontargeted (1)H NMR and GC-MS based metabolomics can successfully identify physiological responses induced by nanoparticles”
t0“Cucumber Plant”
The same questions in other papers
nanoparticle · nutrient: content · Cucumis sativus (cucumber)Positive and negative results
1 paper in the same direction as this paper · 14 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.