Abstract
A simplified technique which cryoprotects zygotic embryos by encapsulation-dehydration was developed for the germplasm conservation of herbaceous peony (Paeonia lactiflora Pall.). The highest survival rate (85%) was obtained from embryos treated by encapsulation-dehydration. The zygotic embryos were precultured on MS medium containing 0.3㎎/l GA₃ for 1 day. The precultured embryos were encapsulated in 3% (w/v) alginate beads and immersed for 1 h in MS medium containing 2 M glycerol and 0.5 M sucrose. The encapsulated embryos were dehydrated for 5 h by air drying prior to direct immersion in liquid nitrogen. This encapsulation-dehydration method appears to be a promising technique for germplasm cryopreservation of a herbaceous peony.
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Claims 2
Claim · model-written summary
Encapsulation dehydration gave the highest embryo survival rate, 85%.
- Taxon
- Paeonia lactiflora Pall.
Paeonia lactiflorat0 - Organ / stage
- embryose0
- Treatment
- encapsulation-dehydratione0
- Outcome
- survival ratee0
- Direction
- higheste0
- Value
- 85%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest survival rate (85%) was obtained from embryos treated by encapsulation-dehydration.”
t0“Paeonia lactiflora Pall.”
Claim · model-written summary
The authors considered encapsulation dehydration promising for peony germplasm cryopreservation.
Verbatim
e0“This encapsulation-dehydration method appears to be a promising technique for germplasm cryopreservation of a herbaceous peony.”