Abstract
Dry seeds from two lines of common bean (Phaseolus vulgaris L.) and one cultivar of faba bean (Vicia faba L.) were germinated on Murashige and Skoog (MS) medium containing B vitamins, 30 g sucrose/liter, and either 2.5, 5.0, or 7.5 μ m benzyladenine (BA). Axenic seed cultures were grown at 22 to 24C in darkness and under continuous light from cool-white fluorescent tubes (40 μmol·m -2 ·s -1 ). Explant tissues were prepared from cotyledonary nodes (CN) and primary nodes (PN) of 14-day-old seedlings. Explants were cultured on corresponding seedling growth medium and maintained under continuous cool-white light (40 μmol·m -2 ·s -1 ). The percentages of CN and PN (in one line of common bean) explants that regenerated shoots and the number of shoots per explant (in all germplasm) were highest when nodal tissues were prepared from seedlings germinated in darkness. These responses were optimal on medium containing 5 μ m BA during seedling growth and subsequent culture of explants. The number of shoots per explant was two to five times higher on explants cultured on medium with 0.25 to 1.0 μ m forchlorfenuron (CPPU) or thidiazuron (TDZ) than on medium with 5 μ m BA. Higher (2.5 and 5 μ m ) CPPU and TDZ concentrations inhibited shoot elongation and stimulated callus production. Histological analyses indicated that adventitious meristems formed 6 to 8 days after explant culture. Progenies from regenerated plants appeared similar to plants raised from the original seed stocks. Chemical names used: N- (phenylmethyl) -1 H- purin-6-amine (benzyladenine, BA); N- (2-chloro-4-pyridyl)- N'- phenylurea (forchlorfenuron, CPPU); N- phenyl -N' -1,2,3-thiadiazol-5-ylurea (thidiazuron, TDZ).
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Claims 11
Claim · model-written summary
Dark seedling germination maximized shoot regeneration percentages of cotyledonary and primary nodes in one common bean line.
- Taxon
- common bean
Phaseolus vulgarise0 - Organ / stage
- CN and PNe0
- Treatment
- germinated in darknesse0
- Outcome
- percentages of CN and PN (in one line of common bean) explants that regenerated shootse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentages of CN and PN (in one line of common bean) explants that regenerated shoots and the number of shoots per explant (in all germplasm) were highest when nodal tissues were prepared from seedlings germinated in darkness.”
Claim · model-written summary
Dark seedling germination maximized shoots per explant across all bean germplasm.
- Taxon
- common beanPhaseolus vulgarise0
- Treatment
- germinated in darknesse0
- Outcome
- number of shoots per explante0
- Direction
- higheste0
- Qualifier
- all germplasme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentages of CN and PN (in one line of common bean) explants that regenerated shoots and the number of shoots per explant (in all germplasm) were highest when nodal tissues were prepared from seedlings germinated in darkness.”
Claim · model-written summary
Responses were optimal with 5 μ m BA during seedling growth and explant culture.
- Taxon
- Common and Faba Beanst0
- Treatment
- medium containing 5 μ m BAe0
- Outcome
- responsese0
- Direction
- optimale0
Not reportedComparator
Verbatim
e0“These responses were optimal on medium containing 5 μ m BA during seedling growth and subsequent culture of explants.”
t0“Common and Faba Beans”
Claim · model-written summary
Low-dose CPPU produced two to five times as many shoots per explant as 5 μ m BA.
Verbatim
e0“The number of shoots per explant was two to five times higher on explants cultured on medium with 0.25 to 1.0 μ m forchlorfenuron (CPPU) or thidiazuron (TDZ) than on medium with 5 μ m BA.”
t0“Common and Faba Beans”
Claim · model-written summary
Low-dose TDZ produced two to five times as many shoots per explant as 5 μ m BA.
Verbatim
e0“The number of shoots per explant was two to five times higher on explants cultured on medium with 0.25 to 1.0 μ m forchlorfenuron (CPPU) or thidiazuron (TDZ) than on medium with 5 μ m BA.”
t0“Common and Faba Beans”
Claim · model-written summary
Higher CPPU concentrations inhibited shoot elongation and stimulated callus production.
- Taxon
- Common and Faba Beanst0
- Treatment
- (2.5 and 5 μ m ) CPPUe0
- Outcome
- shoot elongatione0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Higher (2.5 and 5 μ m ) CPPU and TDZ concentrations inhibited shoot elongation and stimulated callus production.”
t0“Common and Faba Beans”
Claim · model-written summary
Higher TDZ concentrations inhibited shoot elongation.
- Taxon
- Common and Faba Beanst0
- Treatment
- (2.5 and 5 μ m ) CPPU and TDZe0
- Outcome
- shoot elongatione0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Higher (2.5 and 5 μ m ) CPPU and TDZ concentrations inhibited shoot elongation and stimulated callus production.”
t0“Common and Faba Beans”
Claim · model-written summary
Higher CPPU concentrations stimulated callus production.
- Taxon
- Common and Faba Beanst0
- Treatment
- (2.5 and 5 μ m ) CPPUe0
- Outcome
- callus productione0
- Direction
- stimulatede0
Not reportedComparator
Verbatim
e0“Higher (2.5 and 5 μ m ) CPPU and TDZ concentrations inhibited shoot elongation and stimulated callus production.”
t0“Common and Faba Beans”
Claim · model-written summary
Higher TDZ concentrations stimulated callus production.
- Taxon
- Common and Faba Beanst0
- Treatment
- (2.5 and 5 μ m ) CPPU and TDZe0
- Outcome
- callus productione0
- Direction
- stimulatede0
Not reportedComparator
Verbatim
e0“Higher (2.5 and 5 μ m ) CPPU and TDZ concentrations inhibited shoot elongation and stimulated callus production.”
t0“Common and Faba Beans”
Claim · model-written summary
Adventitious meristems formed 6 to 8 days after explant culture.
Verbatim
e0“adventitious meristems formed 6 to 8 days after explant culture.”
t0“Common and Faba Beans”
Claim · model-written summary
Progeny from regenerated bean plants resembled progeny from original seed stocks.
Verbatim
e0“Progenies from regenerated plants appeared similar to plants raised from the original seed stocks.”
t0“Common and Faba Beans”
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