Paper · 2010

In-Vitro Plantlet Regeneration Through Somatic Embryogenesis in Solanum melongena (Thengaithittu Variety)

B. Swamynathan; S. Nadanakunjidam; A. Ramamourti; et al.

9
claims
18
verbatim quotes
11
citations
2
research areas
—
Not open access

Abstract

3 Abstract: The present investigation relates to the study of In vitro regeneration of Solanum melongena (Thengaithittu variety), which is an agronomically important crop grown primarily for its large oval fruit and its medicinal property. The embryo from mature seeds, cotyledon and shoot explants were inoculated on MS medium supplemented with various concentrations and combinations of 1-naphthaleneacetic acid, Kinetin, 2, 4-dichloro phenoxy acetic acid, Thidiazuron and Benzyl amino purine. Cotyledon cultures were highly responsive for callus production on medium supplemented with NAA (10.6 mg/l) alone. However embryo explants cultures showed good responses for callus production and embryogenesis on MS medium supplemented with NAA (8.0mg/l) and Kinetin (0.1mg/l). Callusing response for shoot culture was low. In the presence of NAA, the embryo and cotyledon cultures showed callusing response of 40% and 80% respectively. Callusing response for embryos was comparatively better on 2-4D containing media (45%). Cotyledon explants responded in a similar manner in the presence of 2-4D and NAA (75%). The embryo development occurred in the same medium that contained NAA (8.0mg/l) and Kinetin (0.1mg/l) in two months and a total of 85 embryos were developed. In callus cultures derived from cotyledon explants, a total of 65 embryos were developed in the presence of high concentration of NAA (10.6mg/l) alone. All these embryos derived from cotyledon, embryo and shoot explants were transferred to hormone free medium for germination. After 3-4 weeks the regenerated plantlets were transferred to green house.

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cotyledon cultures were highly responsive for callus production on medium with NAA (10.6 mg/l) alone.

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
Cotyledone1
Treatment
NAA (10.6 mg/l) alonee1
Outcome
callus productione1
Direction
highly responsivee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“Cotyledon cultures were highly responsive for callus production on medium supplemented with NAA (10.6 mg/l) alone”

✓ located verbatim in the source · abstract, characters 496–609 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Embryo explant cultures showed good callus production and embryogenesis on MS medium with NAA (8.0 mg/l) and Kinetin (0.1 mg/l).

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
embryo explantse1
Treatment
NAA (8.0mg/l) and Kinetin (0.1mg/l)e1
Outcome
callus production and embryogenesise1
Direction
good responsese1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“embryo explants cultures showed good responses for callus production and embryogenesis on MS medium supplemented with NAA (8.0mg/l) and Kinetin (0.1mg/l)”

✓ located verbatim in the source · abstract, characters 619–772 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Callusing response of shoot cultures was low.

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
shoote1
Outcome
Callusing responsee1
Direction
lowe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“Callusing response for shoot culture was low”

✓ located verbatim in the source · abstract, characters 774–818 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In the presence of NAA, embryo cultures showed a callusing response of 40%.

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
embryoe1
Treatment
NAAe1
Outcome
callusing responsee1
Value
40%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“In the presence of NAA, the embryo and cotyledon cultures showed callusing response of 40% and 80% respectively”

✓ located verbatim in the source · abstract, characters 820–931 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In the presence of NAA, cotyledon cultures showed a callusing response of 80%.

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
cotyledone1
Treatment
NAAe1
Outcome
callusing responsee1
Value
80%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“In the presence of NAA, the embryo and cotyledon cultures showed callusing response of 40% and 80% respectively”

✓ located verbatim in the source · abstract, characters 820–931 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Callusing response of embryos was comparatively better on 2-4D containing media (45%).

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
embryose1
Treatment
2-4D containing mediae1
Outcome
Callusing responsee1
Direction
bettere1
Value
45%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“Callusing response for embryos was comparatively better on 2-4D containing media (45%)”

✓ located verbatim in the source · abstract, characters 933–1019 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cotyledon explants responded in a similar manner with 2-4D and NAA (75%).

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
Cotyledon explantse1
Treatment
2-4D and NAAe1
Outcome
responded in a similar mannere1
Value
75%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“Cotyledon explants responded in a similar manner in the presence of 2-4D and NAA (75%)”

✓ located verbatim in the source · abstract, characters 1021–1107 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Embryo development occurred on the medium with NAA (8.0 mg/l) and Kinetin (0.1 mg/l) within two months, producing 85 embryos.

Taxon
Solanum melongenaSolanum melongenat0
Treatment
NAA (8.0mg/l) and Kinetin (0.1mg/l)e1
Outcome
embryo developmente1
Value
85 embryose1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“The embryo development occurred in the same medium that contained NAA (8.0mg/l) and Kinetin (0.1mg/l) in two months and a total of 85 embryos were developed”

✓ located verbatim in the source · abstract, characters 1109–1265 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In callus cultures from cotyledon explants, 65 embryos developed with a high concentration of NAA (10.6 mg/l) alone.

Taxon
Solanum melongenaSolanum melongenat0
Organ / stage
cotyledon explantse1
Treatment
NAA (10.6mg/l) alonee1
Outcome
embryos were developede1
Value
65 embryose1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum melongena
Solanum melongena · TaxonBeraDigle · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Solanum melongena”

✓ located verbatim in the source · title, characters 64–81 · show in text ↑
e1

“In callus cultures derived from cotyledon explants, a total of 65 embryos were developed in the presence of high concentration of NAA (10.6mg/l) alone”

✓ located verbatim in the source · abstract, characters 1267–1417 · show in text ↑

kinetin · yield · Solanum melongena (eggplant)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. no effect
See all 2 papers →

1-naphthaleneacetic acid · yield · Solanum melongena (eggplant)Results agree

18 papers: 18 positive, 0 negative or no effect

17 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

14 papers more in this group

See all 18 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.