Abstract
A protocol to culture nodal explants of Rosa hybrida cv. 'Heirloom' in vitro was developed. Multiple shoot formation of up to 3 shoots were obtained on Murashige and Skoog (MS) medium supplemented with 13.3 mM BA and 9.3 mM kinetin. Regenerated shoots cultured on MS medium containing various concentrations of BA, kinetin, and sucrose did not flower. Flower induction occurred when regenerated shoots were cultured on MS medium supplemented with 13.3 mM BA and 9.3 mM kinetin under photoperiod of 12/12 (light/dark cycle). The 3-week intervals for two consecutive subcultures on this medium were efficient for flower induction. Shoots readily rooted on 1/4 MS medium were found to be devoid of growth regulators. Rooted plantlets were hardened and established in pots at 100% survival.
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Claims 6
Claim · model-written summary
Nodal explants of Rosa hybrida cv. 'Heirloom' formed up to 3 shoots in vitro on MS medium with 13.3 mM BA and 9.3 mM kinetin.
- Taxon
- Rosa hybrida
Rosa hybrid cultivare0 - Material
- cv. 'Heirloom'e0
- Organ / stage
- nodal explantse0
- Treatment
- 13.3 mM BA and 9.3 mM kinetine1
- Outcome
- Multiple shoot formatione1
- Value
- up to 3 shootse1
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A protocol to culture nodal explants of Rosa hybrida cv. 'Heirloom' in vitro was developed”
e1“Multiple shoot formation of up to 3 shoots were obtained on Murashige and Skoog (MS) medium supplemented with 13.3 mM BA and 9.3 mM kinetin”
Claim · model-written summary
Regenerated Rosa hybrida shoots did not flower on MS medium with various concentrations of BA, kinetin and sucrose.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Organ / stage
- Regenerated shootse2
- Treatment
- various concentrations of BA, kinetin, and sucrosee2
- Outcome
- flowere2
- Qualifier
- did not flowere2
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A protocol to culture nodal explants of Rosa hybrida cv. 'Heirloom' in vitro was developed”
e2“Regenerated shoots cultured on MS medium containing various concentrations of BA, kinetin, and sucrose did not flower”
Claim · model-written summary
Flower induction occurred in regenerated Rosa hybrida shoots cultured on MS medium with 13.3 mM BA and 9.3 mM kinetin under a 12/12 photoperiod.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- 13.3 mM BA and 9.3 mM kinetin under photoperiod of 12/12e3
- Outcome
- Flower inductione3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A protocol to culture nodal explants of Rosa hybrida cv. 'Heirloom' in vitro was developed”
e3“Flower induction occurred when regenerated shoots were cultured on MS medium supplemented with 13.3 mM BA and 9.3 mM kinetin under photoperiod of 12/12 (light/dark cycle)”
Claim · model-written summary
Two consecutive 3-week subcultures on this medium were efficient for Rosa hybrida flower induction.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- 3-week intervals for two consecutive subculturese4
- Outcome
- flower inductione4
- Qualifier
- efficiente4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A protocol to culture nodal explants of Rosa hybrida cv. 'Heirloom' in vitro was developed”
e4“The 3-week intervals for two consecutive subcultures on this medium were efficient for flower induction”
Claim · model-written summary
Shoots of Rosa hybrida rooted readily on 1/4 MS medium without growth regulators.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Treatment
- 1/4 MS mediume5
- Outcome
- rootede5
- Qualifier
- readilye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A protocol to culture nodal explants of Rosa hybrida cv. 'Heirloom' in vitro was developed”
e5“Shoots readily rooted on 1/4 MS medium were found to be devoid of growth regulators”
Claim · model-written summary
Rooted Rosa hybrida plantlets, hardened and established in pots, had 100% survival.
- Taxon
- Rosa hybridaRosa hybrid cultivare0
- Outcome
- survivale6
- Value
- 100%e6
- Setting
- potse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A protocol to culture nodal explants of Rosa hybrida cv. 'Heirloom' in vitro was developed”
e6“Rooted plantlets were hardened and established in pots at 100% survival”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.