Abstract
To determine the best growing conditions for in vitro culture of different genotypes of fennel to mass production, we compared different explants in various culture mediums for eight fennel genotype groups. Based on completely randomized plan, factorial experiment was done with four replications. Leaf, petiole, hypocotyl and root explants were isolated for 20 days, and seedlings were cultured on callus induction medium, 1/2 MS basal medium with 2,4-dichlorophenoxyacetic acid (2, 4-D) (2, 4 and 6 mg l -1 ) and 6-benzyaminopurine (BAP) (0.5, 0.25 and 0.1 mg l -1 ) combination. Color, Size, Percentage of callus induction, dry and fresh weight and Volume of callus were evaluated 3 month later. Through most of the genotypes, Hypocotyls and petiole explants in callus induction were better than the others. Callus induction mediums with 6 mgl -1 2-4-D and BAP combinations had more appropriate responses. Leaf explants showed the highest callus size in the German genotype in callus induction medium with 4mgl-1 2-4-D and 0.1mgl-1 BAP. Embryogenic calli were placed on regeneration medium with 1/2MS of a non-hormonal treatment and hormonal treatment with a level of 2-4-D (10 mgl -1 ) and two levels of BAP (2 and 5 mgl -1 ). The medium without hormones was recognized as the best medium for regeneration and hypocotyl and petiole had the highest rate of regeneration. Respectively, Tabriz
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Claims 5
Claim · model-written summary
Hypocotyl and petiole explants gave better callus induction than the other explants in most fennel genotypes.
- Taxon
- fennel
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=60)
Anethum foeniculume0 - Organ / stage
- Hypocotyls and petiolee1
- Treatment
- Hypocotyls and petiole explantse1
- Outcome
- callus inductione1
- Direction
- bettere1
- Qualifier
- Through most of the genotypese1
- Comparator
- the otherse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=500)
Verbatim
e1“Through most of the genotypes, Hypocotyls and petiole explants in callus induction were better than the others”
e0“we compared different explants in various culture mediums for eight fennel genotype groups”
Claim · model-written summary
Callus induction medium with 6 mg/l 2,4-D and BAP combinations gave more appropriate responses.
- Taxon
- fennelAnethum foeniculume0
- Treatment
- 6 mgl -1 2-4-D and BAP combinationse2
- Outcome
- appropriate responsese2
- Direction
- more appropriatee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Callus induction mediums with 6 mgl -1 2-4-D and BAP combinations had more appropriate responses”
e0“we compared different explants in various culture mediums for eight fennel genotype groups”
Claim · model-written summary
Leaf explants gave the highest callus size in the German fennel genotype in callus induction medium with 4 mg/l 2,4-D and 0.1 mg/l BAP.
- Taxon
- fennelAnethum foeniculume0
- Material
- German genotypee3
- Organ / stage
- Leaf explantse3
- Treatment
- callus induction medium with 4mgl-1 2-4-D and 0.1mgl-1 BAPe3
- Outcome
- callus sizee3
- Direction
- higheste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Leaf explants showed the highest callus size in the German genotype in callus induction medium with 4mgl-1 2-4-D and 0.1mgl-1 BAP”
e0“we compared different explants in various culture mediums for eight fennel genotype groups”
Claim · model-written summary
The medium without hormones was the best medium for regeneration in fennel.
- Taxon
- fennelAnethum foeniculume0
- Treatment
- medium without hormonese4
- Outcome
- regeneratione4
- Direction
- beste4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The medium without hormones was recognized as the best medium for regeneration”
e0“we compared different explants in various culture mediums for eight fennel genotype groups”
Claim · model-written summary
Hypocotyl and petiole explants had the highest rate of regeneration in fennel.
- Taxon
- fennelAnethum foeniculume0
- Organ / stage
- hypocotyl and petiolee5
- Outcome
- rate of regeneratione5
- Direction
- higheste5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“hypocotyl and petiole had the highest rate of regeneration”
e0“we compared different explants in various culture mediums for eight fennel genotype groups”
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1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.