Abstract
Hypocotyl explants of Brassica napus L. cv. Dunkled were used to investigate the effects of various plant growth regulators and silver nitrate on morphogenesis in vitro. Two types of explants, such as upper and lower portion of hypocotyl were examined. A high frequency of shoot regeneration (96 %) was achieved on MS supplemented with growth regulators. Shoots regenerated earlier (within three weeks) from segments taken from the upper portion of the hypocotyl than those from lower portion (after three weeks), although shoot regeneration frequency was similar in both the cases. Exclusion of silver nitrate from the medium drastically reduced the regeneration potential (50 %). Multiple shoot regeneration was obtained from the periodic subculture of the explants in the same medium. In vitro elongated shoots were rooted with a 100 % success by treating them with half-strength MS supplemented with 0.250 mg/l IBA and 0.125 mg/l IAA. Rooted plantlets were successfully established into the soil.
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Claims 7
Claim · model-written summary
A high frequency of shoot regeneration (96 %) was achieved on MS supplemented with growth regulators.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Material
- Dunklede1
- Organ / stage
- hypocotyl explantse1
- Treatment
- MS supplemented with growth regulatorse3
- Outcome
- shoot regeneratione3
- Value
- 96 %e3
- Setting
- in vitroe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e1“Hypocotyl explants of Brassica napus L. cv. Dunkled”
e2“on morphogenesis in vitro”
e3“A high frequency of shoot regeneration (96 %) was achieved on MS supplemented with growth regulators”
Claim · model-written summary
Shoots regenerated earlier from upper hypocotyl segments than from lower hypocotyl segments.
- Taxon
- Brassica napusBrassica napuse1
- Material
- Dunklede1
- Treatment
- segments taken from the upper portion of the hypocotyle4
- Outcome
- Shoots regeneratede4
- Direction
- earliere4
- Value
- within three weekse4
- Comparator
- lower portione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Hypocotyl explants of Brassica napus L. cv. Dunkled”
e4“Shoots regenerated earlier (within three weeks) from segments taken from the upper portion of the hypocotyl than those from lower portion (after three weeks)”
Claim · model-written summary
Shoot regeneration frequency was similar for upper and lower hypocotyl segments.
- Taxon
- Brassica napusBrassica napuse1
- Material
- Dunklede1
- Treatment
- segments taken from the upper portion of the hypocotyle4
- Outcome
- shoot regeneration frequencye5
- Direction
- similare5
- Comparator
- lower portione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Hypocotyl explants of Brassica napus L. cv. Dunkled”
e4“Shoots regenerated earlier (within three weeks) from segments taken from the upper portion of the hypocotyl than those from lower portion (after three weeks)”
e5“although shoot regeneration frequency was similar in both the cases”
Claim · model-written summary
Excluding silver nitrate from the medium reduced shoot regeneration potential to 50 %.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- Exclusion of silver nitrate from the mediume6
- Outcome
- regeneration potentiale6
- Direction
- reducede6
- Value
- 50 %e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Hypocotyl explants of Brassica napus L. cv. Dunkled”
e6“Exclusion of silver nitrate from the medium drastically reduced the regeneration potential (50 %)”
Claim · model-written summary
Multiple shoot regeneration was obtained from periodic subculture of the explants in the same medium.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- periodic subculture of the explants in the same mediume7
- Outcome
- Multiple shoot regeneratione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Hypocotyl explants of Brassica napus L. cv. Dunkled”
e7“Multiple shoot regeneration was obtained from the periodic subculture of the explants in the same medium”
Claim · model-written summary
Half-strength MS medium with 0.250 mg/l IBA and 0.125 mg/l IAA rooted in vitro elongated shoots with 100 % success.
- Taxon
- Brassica napusBrassica napuse1
- Organ / stage
- elongated shootse2
- Treatment
- half-strength MS supplemented with 0.250 mg/l IBA and 0.125 mg/l IAAe2
- Outcome
- rootede2
- Value
- 100 %e2
- Setting
- In vitroe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Hypocotyl explants of Brassica napus L. cv. Dunkled”
e2“In vitro elongated shoots were rooted with a 100 % success by treating them with half-strength MS supplemented with 0.250 mg/l IBA and 0.125 mg/l IAA”
Claim · model-written summary
Rooted plantlets were successfully established in soil.
- Taxon
- Brassica napusBrassica napuse1
- Organ / stage
- Rooted plantletse2
- Outcome
- successfully establishede2
- Qualifier
- into the soile2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Hypocotyl explants of Brassica napus L. cv. Dunkled”
e2“Rooted plantlets were successfully established into the soil”
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See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.