Abstract
The present paper deals with the effect of different concentrations (1, 2, 5, 10 and 15 μM) of 6- benzyladenine (BA) and thidiazuron (TDZ), combined with indole-3-butyric acid (IBA) at concentrations of 0, 0.5, 2.5 and 4.9 μM on in vitro multiplication of the pear rootstock 'Pyrodwarf'. Murashige and Skoog (1962) (MS) was the basal medium used in all combinations. The multiplication index as well as the length of axial and lateral shoots were monitored. The highest multiplication index (2.89) was obtained on the medium supplemented with 5 μM BA and 0.5 μM IBA. Higher concentrations of TDZ (more than 2 μM TDZ), in combination with IBA, brought about fasciation of shoots, which was confirmed by SEM technique. On the rooting medium consisting in 1/2 MS supplemented with 5 μM IBA and 0.3 μM giberellic acid (GA3), the higher rooting percentage and other superior rooting parameters were also recorded in the case of the shoots originated from BA media. The obtained results suggest that to gain good multiplication parameters it is recommended to use good quality shoots for further subcultivation. In order to avoid the occurrence of fasciation it is required to use the cytokinin BA or to limit the concentration of TDZ in the multiplication phase of pear 'Pyrodwarf' rootstock.
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Claims 7
Claim · model-written summary
The highest Pyrodwarf multiplication index, 2.89, occurred with 5 μM BA and 0.5 μM IBA.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise1 - Material
- 'Pyrodwarf'e1
- Treatment
- 5 μM BA and 0.5 μM IBAe0
- Outcome
- multiplication indexe0
- Direction
- higheste0
- Value
- 2.89e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“The highest multiplication index (2.89) was obtained on the medium supplemented with 5 μM BA and 0.5 μM IBA.”
e1“pear rootstock 'Pyrodwarf'”
Claim · model-written summary
TDZ concentrations above 2 μM combined with IBA caused shoot fasciation.
- Taxon
- pearPyrus communise1
- Material
- 'Pyrodwarf'e1
- Treatment
- more than 2 μM TDZ), in combination with IBAe0
- Outcome
- fasciation of shootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher concentrations of TDZ (more than 2 μM TDZ), in combination with IBA, brought about fasciation of shoots”
e1“pear rootstock 'Pyrodwarf'”
Claim · model-written summary
Shoots originating from BA media had a higher rooting percentage on the specified rooting medium.
- Taxon
- pearPyrus communise1
- Material
- 'Pyrodwarf'e1
- Treatment
- shoots originated from BA mediae0
- Outcome
- rooting percentagee0
- Direction
- highere0
- Setting
- rooting medium consisting in 1/2 MS supplemented with 5 μM IBA and 0.3 μM giberellic acid (GA3)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the rooting medium consisting in 1/2 MS supplemented with 5 μM IBA and 0.3 μM giberellic acid (GA3), the higher rooting percentage and other superior rooting parameters were also recorded in the case of the shoots originated from BA media.”
e1“pear rootstock 'Pyrodwarf'”
Claim · model-written summary
Shoots originating from BA media also had superior other rooting parameters.
- Taxon
- pearPyrus communise1
- Material
- 'Pyrodwarf'e1
- Treatment
- shoots originated from BA mediae0
- Outcome
- other superior rooting parameterse0
- Direction
- superiore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the higher rooting percentage and other superior rooting parameters were also recorded in the case of the shoots originated from BA media.”
e1“pear rootstock 'Pyrodwarf'”
Claim · model-written summary
The authors recommend good quality shoots for further subcultivation.
- Taxon
- pearPyrus communise1
- Outcome
- use good quality shoots for further subcultivatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is recommended to use good quality shoots for further subcultivation.”
e1“pear rootstock 'Pyrodwarf'”
Claim · model-written summary
The authors recommend BA during Pyrodwarf multiplication to avoid fasciation.
- Taxon
- pearPyrus communise0
- Material
- 'Pyrodwarf'e0
- Treatment
- cytokinin BAe0
- Outcome
- use the cytokinin BAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In order to avoid the occurrence of fasciation it is required to use the cytokinin BA or to limit the concentration of TDZ in the multiplication phase of pear 'Pyrodwarf' rootstock.”
Claim · model-written summary
The authors recommend limiting TDZ concentration during Pyrodwarf multiplication to avoid fasciation.
- Taxon
- pearPyrus communise0
- Material
- 'Pyrodwarf'e0
- Outcome
- limit the concentration of TDZe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In order to avoid the occurrence of fasciation it is required to use the cytokinin BA or to limit the concentration of TDZ in the multiplication phase of pear 'Pyrodwarf' rootstock.”
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