Abstract
Abstract Arachis hypogaea L. cv. NC 4 was used as a model plant system in an effort to develop an in vitro embryo rescue protocol which could have application to interspecific hybrid embryos, which often abort at very early growth stages. Embryo growth and development was studied in 1- to 4-day-old peg tips containing proembryos equivalent to a stage where many interspecific hybrid embryos abort. Three independent experiments were conducted to 1) determine the most favorable basal media, 2) evaluate the effects of auxins and cytokinins on growth, and 3) determine a favorable combination of auxins and cytokinins for in vitro peanut embryo growth. The results indicated that MS (Murashige and Skoog) medium with 3% sucrose was the most favorable basal medium among seven media and two sucrose concentrations analyzed. IAA (indole-3-acetic acid) at 1.5 mg L-1 in combination with a range of KN (kinetin) levels from 0.5 to 1.25 mg L-1 were the growth regulator combinations of choice. Proembryo growth reached the multicellular globular stage, but differentiation into heart-shaped embryos did not occur.
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Claims 4
Claim · model-written summary
MS medium with 3% sucrose was the most favorable basal medium among seven media and two sucrose concentrations for in vitro peanut embryo growth.
- Taxon
- Arachis hypogaea
Arachis hypogaeae0 - Material
- cv. NC 4e0
- Treatment
- MS (Murashige and Skoog) medium with 3% sucrosee1
- Outcome
- most favorable basal mediume1
- Direction
- most favorablee1
- Comparator
- seven media and two sucrose concentrationse1
- Setting
- In Vitrot0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arachis hypogaea L. cv. NC 4”
e1“MS (Murashige and Skoog) medium with 3% sucrose was the most favorable basal medium among seven media and two sucrose concentrations analyzed”
t0“In Vitro Culture of Arachis hypogaea Peg Tips1”
Claim · model-written summary
IAA at 1.5 mg L-1 combined with kinetin at 0.5 to 1.25 mg L-1 were the growth regulator combinations of choice for proembryo growth.
- Taxon
- Arachis hypogaeaArachis hypogaeae0
- Treatment
- IAA (indole-3-acetic acid) at 1.5 mg L-1 in combination with a range of KN (kinetin) levels from 0.5 to 1.25 mg L-1e2
- Outcome
- growth regulator combinations of choicee2
- Direction
- of choicee2
- Setting
- In Vitrot0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arachis hypogaea L. cv. NC 4”
e2“IAA (indole-3-acetic acid) at 1.5 mg L-1 in combination with a range of KN (kinetin) levels from 0.5 to 1.25 mg L-1 were the growth regulator combinations of choice”
t0“In Vitro Culture of Arachis hypogaea Peg Tips1”
Claim · model-written summary
Proembryo growth reached the multicellular globular stage in vitro.
- Taxon
- Arachis hypogaeaArachis hypogaeae0
- Organ / stage
- proembryoe3
- Outcome
- growthe3
- Qualifier
- multicellular globular stagee3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arachis hypogaea L. cv. NC 4”
e3“Proembryo growth reached the multicellular globular stage, but differentiation into heart-shaped embryos did not occur”
Claim · model-written summary
Differentiation of proembryos into heart-shaped embryos did not occur in vitro.
- Taxon
- Arachis hypogaeaArachis hypogaeae0
- Organ / stage
- proembryoe3
- Outcome
- differentiation into heart-shaped embryose3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arachis hypogaea L. cv. NC 4”
e3“Proembryo growth reached the multicellular globular stage, but differentiation into heart-shaped embryos did not occur”
The same questions in other papers
kinetin · growth · Arachis hypogaea (peanut)Results agree
2 papers in the same direction as this paper
- positive
- positive
indole-3-acetic acid · growth · Arachis hypogaea (peanut)Results agree
7 papers in the same direction as this paper
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.