Abstract
Seeds of Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid) were encapsulated in alginate beads with hyphae of the basidomycete fungus Ceratobasidium cornigerum. Pre-treatment of beads for 18 h with sucrose at an optimum concentration of 0.75 M decreased the desiccation rate in a flow of sterile air (c. 23 degree C, 30% RH) and increased seed and fungal survival after up to 16 h drying. Pre-treated and 16-h dried beads were transferred to cryo-vials and subsequently stored at a range of low temperatures for up to 30 d. Neither embryo growth of both orchids nor fungal development was detrimentally affected by 1 d storage at -196 degree C when the beads were pre-dried to c. 20% moisture content. Encapsulated D. fuchsii seed and compatible fungus had < 5% and < 45% viability when beads of the same moisture content were stored for 1 d at -20 degree C and -70 degree C respectively. In contrast, viability of the seed and the fungus remained unchanged during 30 days storage at -196 degree C but was progressively lost at 16 degree C over the same interval. The results indicate opportunities for the use of simultaneous cryopreservation as a conservation tool for diverse taxa.
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Claims 11
Claim · model-written summary
Sucrose pretreatment decreased bead desiccation rate.
- Taxon
- Dactylorhiza fuchsii
Dactylorhiza fuchsiie1 - Treatment
- sucrose at an optimum concentration of 0.75 Me0
- Outcome
- desiccation ratee0
- Direction
- decreasede0
- Setting
- c. 23 degree C, 30% RHe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“Pre-treatment of beads for 18 h with sucrose at an optimum concentration of 0.75 M decreased the desiccation rate in a flow of sterile air (c. 23 degree C, 30% RH)”
Claim · model-written summary
Sucrose pretreatment increased seed survival after drying.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- sucrose at an optimum concentration of 0.75 Me0
- Outcome
- seed and fungal survivale0
- Direction
- increasede0
- Qualifier
- up to 16 h dryinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“Pre-treatment of beads for 18 h with sucrose at an optimum concentration of 0.75 M decreased the desiccation rate in a flow of sterile air (c. 23 degree C, 30% RH) and increased seed and fungal survival after up to 16 h drying.”
Claim · model-written summary
Sucrose pretreatment increased fungal survival after drying.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- sucrosee0
- Interactor
- Ceratobasidium cornigerum
.jpg?width=60)
Ceratobasidium cornigerume2symbiont - Outcome
- fungal survivale0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“Pre-treatment of beads for 18 h with sucrose at an optimum concentration of 0.75 M decreased the desiccation rate in a flow of sterile air (c. 23 degree C, 30% RH) and increased seed and fungal survival after up to 16 h drying.”
Claim · model-written summary
Cryostorage for one day did not harm embryo growth in D. fuchsii.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- 1 d storage at -196 degree Ce0
- Outcome
- embryo growthe0
- Direction
- Neithere0
- Qualifier
- c. 20% moisture contente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“Neither embryo growth of both orchids nor fungal development was detrimentally affected by 1 d storage at -196 degree C when the beads were pre-dried to c. 20% moisture content.”
Claim · model-written summary
Cryostorage for one day did not harm embryo growth in A. morio.
- Taxon
- Anacamptis morio
Anacamptis morioe1 - Treatment
- 1 d storage at -196 degree Ce0
- Outcome
- embryo growthe0
- Direction
- Neithere0
- Qualifier
- c. 20% moisture contente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“Neither embryo growth of both orchids nor fungal development was detrimentally affected by 1 d storage at -196 degree C when the beads were pre-dried to c. 20% moisture content.”
Claim · model-written summary
One day of cryostorage did not harm fungal development.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- 1 d storage at -196 degree Ce0
- Interactor
- Ceratobasidium cornigerumCeratobasidium cornigerume2symbiont
- Outcome
- fungal developmente0
- Direction
- Neithere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“Neither embryo growth of both orchids nor fungal development was detrimentally affected by 1 d storage at -196 degree C when the beads were pre-dried to c. 20% moisture content.”
Claim · model-written summary
At -20°C, D. fuchsii seed viability was below 5%.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- 1 d at -20 degree Ce0
- Outcome
- D. fuchsii seede0
- Value
- < 5%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“Encapsulated D. fuchsii seed and compatible fungus had < 5% and < 45% viability when beads of the same moisture content were stored for 1 d at -20 degree C and -70 degree C respectively.”
Claim · model-written summary
At -70°C, compatible fungus viability was below 45%.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- -70 degree Ce0
- Interactor
- Ceratobasidium cornigerumCeratobasidium cornigerume2symbiont
- Outcome
- compatible funguse0
- Value
- < 45%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“Encapsulated D. fuchsii seed and compatible fungus had < 5% and < 45% viability when beads of the same moisture content were stored for 1 d at -20 degree C and -70 degree C respectively.”
Claim · model-written summary
Seed viability remained unchanged for 30 days at -196°C.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- 30 days storage at -196 degree Ce0
- Outcome
- viability of the seede0
- Direction
- remained unchangede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“viability of the seed and the fungus remained unchanged during 30 days storage at -196 degree C”
Claim · model-written summary
Fungal viability remained unchanged for 30 days at -196°C.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- 30 days storage at -196 degree Ce0
- Interactor
- Ceratobasidium cornigerumCeratobasidium cornigerume2symbiont
- Outcome
- the funguse0
- Direction
- remained unchangede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“viability of the seed and the fungus remained unchanged during 30 days storage at -196 degree C”
Claim · model-written summary
Seed and fungal viability progressively declined at 16°C over 30 days.
- Taxon
- Dactylorhiza fuchsiiDactylorhiza fuchsiie1
- Treatment
- 16 degree Ce0
- Interactor
- Ceratobasidium cornigerumCeratobasidium cornigerume2symbiont
- Outcome
- viability of the seed and the funguse0
- Direction
- progressively loste0
- Comparator
- -196 degree Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Dactylorhiza fuchsii (common spotted orchid) and Anacamptis morio (green-winged orchid)”
e2“hyphae of the basidomycete fungus Ceratobasidium cornigerum”
e0“viability of the seed and the fungus remained unchanged during 30 days storage at -196 degree C but was progressively lost at 16 degree C over the same interval.”
The same questions in other papers
storage · growth · Dactylorhiza fuchsiiResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.