Abstract
Gibberellin (GA) regulates growth and development in plants. We isolated and characterized a rice GA-insensitive dwarf mutant, gid2. The GID2 gene encodes a putative F-box protein, which interacted with the rice Skp1 homolog in a yeast two-hybrid assay. In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type. We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCF(GID2)-proteasome pathway.
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Claims 8
Claim · model-written summary
The rice gid2 mutant was insensitive to gibberellin and dwarf.
- Taxon
- rice
Oryza sativae0 - Material
- gid2e0
- Outcome
- GA-insensitive dwarf mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice GA-insensitive dwarf mutant, gid2”
Claim · model-written summary
GID2 encodes a putative F-box protein.
- Taxon
- riceOryza sativae1
- Gene
- GID2e0
- Outcome
- putative F-box proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GID2 gene encodes a putative F-box protein”
e1“rice GA-insensitive dwarf mutant, gid2”
Claim · model-written summary
GID2 interacted with the rice Skp1 homolog in a yeast two-hybrid assay.
- Taxon
- riceOryza sativat0e0
- Gene
- rice Skp1 homologe0
- Outcome
- interactede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which interacted with the rice Skp1 homolog in a yeast two-hybrid assay”
t0“rice Skp1 homolog”
Claim · model-written summary
SLR1 accumulated highly in phosphorylated form in gid2.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form”
e1“rice GA-insensitive dwarf mutant, gid2”
Claim · model-written summary
GA increased SLR1 concentration in gid2.
- Taxon
- riceOryza sativae2
- Material
- gid2e1
- Treatment
- GAe0
- Gene
- SLR1e1
- Outcome
- concentratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA increased its concentration”
e1“In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form”
e2“rice GA-insensitive dwarf mutant, gid2”
Claim · model-written summary
GA rapidly degraded SLR1 through ubiquitination in wild type.
- Taxon
- riceOryza sativae1
- Treatment
- GAe0
- Gene
- SLR1e0
- Outcome
- SLR1e0
- Direction
- rapidly degradede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SLR1 was rapidly degraded by GA through ubiquitination in the wild type”
e1“rice GA-insensitive dwarf mutant, gid2”
Claim · model-written summary
The authors conclude that GID2 positively regulates GA signaling.
- Taxon
- riceOryza sativae1
- Gene
- GID2e0
- Outcome
- GA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GID2 is a positive regulator of GA signaling”
e1“rice GA-insensitive dwarf mutant, gid2”
Claim · model-written summary
The authors conclude that GA-dependent phosphorylation initiates SLR1 degradation and an SCF(GID2)-proteasome pathway finalizes it.
- Taxon
- riceOryza sativae1
- Gene
- SLR1e0
- Outcome
- regulated degradation of SLR1e0
- Qualifier
- GA-dependent phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCF(GID2)-proteasome pathway”
e1“rice GA-insensitive dwarf mutant, gid2”
The same questions in other papers
Gibberellic acid · molecular: metabolites · Oryza sativa (rice)Positive and negative results
11 in the opposite direction
- positive
- positive
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.