Abstract
Several Bacillus strains belonging to the B. subtilis/amyloliquefaciens group isolated from plant-pathogen-infested soil possess plant-growth-promoting activity [Krebs, B. et al. (1998) J Plant Dis Prot 105, 181-197]. Three out of the four strains investigated were identified as B. amyloliquefaciens and were able to degrade extracellular phytate (myo-inositol hexakisphosphate). The highest extracellular phytase activity was detected in strain FZB45, and diluted culture filtrates of this strain stimulated growth of maize seedlings under phosphate limitation in the presence of phytate. The amino acid sequence deduced from the phytase phyA gene cloned from FZB45 displayed a high degree of similarity to known Bacillus phytases. Weak similarity between FZB45 phytase and B. subtilis alkaline phosphatase IV pointed to a possible common origin of these two enzymes. The recombinant protein expressed by B. subtilis MU331 displayed 3(1)-phytase activity yielding D/L-Ins(1,2,4,5,6)P5 as the first product of phytate hydrolysis. A phytase-negative mutant strain, FZB45/M2, whose phyA gene is disrupted, was generated by replacing the entire wild-type gene on the chromosome of FZB45 with a km::phyA fragment, and culture filtrates obtained from FZB45/M2 did not stimulate plant growth. In addition, the growth of maize seedlings was promoted in the presence of purified phytase and the absence of culture filtrate. These genetic and biochemical experiments provide strong evidence that phytase activity of B. amyloliquefaciens FZB45 is important for plant growth stimulation under phosphate limitation.
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Claims 9
Claim · model-written summary
Three of four Bacillus strains were identified as B. amyloliquefaciens and could degrade phytate.
- Interactor
- B. amyloliquefaciens
.jpg?width=60)
Bacillus amyloliquefaciense0symbionte1 - Outcome
- degrade extracellular phytatee0
- Value
- Three out of the foure0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Three out of the four strains investigated were identified as B. amyloliquefaciens and were able to degrade extracellular phytate (myo-inositol hexakisphosphate).”
t0“growth of maize seedlings under phosphate limitation”
e1“plant-growth-promoting activity”
Claim · model-written summary
FZB45 had the highest extracellular phytase activity.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest extracellular phytase activity was detected in strain FZB45”
t0“growth of maize seedlings under phosphate limitation”
e1“plant-growth-promoting activity”
Claim · model-written summary
Diluted FZB45 culture filtrates stimulated maize seedling growth under phosphate limitation with phytate.
- Treatment
- diluted culture filtratese0
- Interactor
- FZB45e1symbiont
- Outcome
- growthe0
- Direction
- stimulatede0
- Qualifier
- under phosphate limitation in the presence of phytatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“diluted culture filtrates of this strain stimulated growth of maize seedlings under phosphate limitation in the presence of phytate.”
e1“strain FZB45”
Claim · model-written summary
FZB45 phyA had high sequence similarity to known Bacillus phytases.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amino acid sequence deduced from the phytase phyA gene cloned from FZB45 displayed a high degree of similarity to known Bacillus phytases.”
t0“growth of maize seedlings under phosphate limitation”
e1“plant-growth-promoting activity”
Claim · model-written summary
FZB45 phytase showed weak similarity to B. subtilis alkaline phosphatase IV.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Weak similarity between FZB45 phytase and B. subtilis alkaline phosphatase IV pointed to a possible common origin of these two enzymes.”
t0“growth of maize seedlings under phosphate limitation”
e1“plant-growth-promoting activity”
Claim · model-written summary
Recombinant protein displayed 3(1)-phytase activity and yielded D/L-Ins(1,2,4,5,6)P5 first.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recombinant protein expressed by B. subtilis MU331 displayed 3(1)-phytase activity yielding D/L-Ins(1,2,4,5,6)P5 as the first product of phytate hydrolysis.”
t0“growth of maize seedlings under phosphate limitation”
Claim · model-written summary
FZB45/M2 filtrates did not stimulate plant growth.
- Treatment
- culture filtrates obtained from FZB45/M2e0
- Outcome
- plant growthe0
- Direction
- did not stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“culture filtrates obtained from FZB45/M2 did not stimulate plant growth.”
t0“growth of maize seedlings under phosphate limitation”
e1“plant-growth-promoting activity”
Claim · model-written summary
Purified phytase promoted maize seedling growth without culture filtrate.
- Treatment
- purified phytasee0
- Outcome
- growthe0
- Direction
- promotede0
- Comparator
- absence of culture filtratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the growth of maize seedlings was promoted in the presence of purified phytase and the absence of culture filtrate.”
Claim · model-written summary
The authors conclude FZB45 phytase activity is important for growth stimulation under phosphate limitation.
- Interactor
- B. amyloliquefaciens FZB45Bacillus amyloliquefaciense0symbionte1
- Gene
- phytasee0
- Outcome
- plant growth stimulatione0
- Qualifier
- under phosphate limitatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phytase activity of B. amyloliquefaciens FZB45 is important for plant growth stimulation under phosphate limitation.”
t0“growth of maize seedlings under phosphate limitation”
e1“plant-growth-promoting activity”
The same questions in other papers
pathogen product · growth · Zea mays (maize)Positive and negative results
11 papers in the same direction as this paper · 12 in the opposite direction
- negative
- negative
- no effect
20 papers more in this group
See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.