Abstract
Tomato ringspot virus (ToRSV, a Nepovirus sp.) systemically infects many herbaceous plants. Viral RNA accumulates in symptomatic leaves and in young, asymptomatic leaves that emerge late in infection. Here, we show that systemic infection by ToRSV is restricted in tobacco. After an initial hypersensitive response in inoculated leaves, only a few plants showed limited systemic symptoms. Viral RNA did not usually accumulate to detectable levels in asymptomatic leaves. ToRSV-derived small-interfering RNAs and PR1a transcripts were only detected in tissues that contained viral RNA, indicating local induction of RNA silencing and salicylic acid (SA)-dependent defense responses. Lesion size and viral systemic spread were reduced with SA pretreatment but enhanced in NahG transgenic lines deficient in SA accumulation, suggesting that SA-dependent mechanisms play a key role in limiting ToRSV spread in tobacco. Restriction of virus infection was enhanced in transgenic lines expressing the P1-HC-Pro suppressor of silencing. Knocking down the SA-inducible RNA-dependent RNA polymerase 1 exacerbated the necrotic reaction but did not affect viral systemic spread. ToRSV-infected tobacco plants were susceptible to reinoculation by ToRSV or Tobacco mosaic virus, although a small reduction in lesion size was observed. This moderate systemic resistance suggests inefficient induction or spread of RNA silencing and systemic acquired resistance signal molecules.
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Claims 13
Claim · model-written summary
Systemic infection by ToRSV is restricted in tobacco, with only a few plants showing limited systemic symptoms after an initial hypersensitive response.
- Taxon
- tobacco
Nicotiana tabacume0 - Interactor
- ToRSVe0pathogen
- Outcome
- systemic infection by ToRSVe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e1“After an initial hypersensitive response in inoculated leaves, only a few plants showed limited systemic symptoms”
Claim · model-written summary
Viral RNA did not usually accumulate to detectable levels in asymptomatic leaves of ToRSV-infected tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- asymptomatic leavese2
- Outcome
- accumulate to detectable levelse2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e2“Viral RNA did not usually accumulate to detectable levels in asymptomatic leaves”
Claim · model-written summary
ToRSV-derived small-interfering RNAs and PR1a transcripts were detected only in tissues that contained viral RNA.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- tissues that contained viral RNAe3
- Interactor
- ToRSVe3pathogen
- Outcome
- ToRSV-derived small-interfering RNAs and PR1a transcriptse3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e3“ToRSV-derived small-interfering RNAs and PR1a transcripts were only detected in tissues that contained viral RNA”
Claim · model-written summary
Local induction of RNA silencing and SA-dependent defence responses occurs in tobacco infected with ToRSV.
- Taxon
- tobaccoNicotiana tabacume0
- Compound
- salicylic acid (SA)e4
- Outcome
- local induction of RNA silencing and salicylic acid (SA)-dependent defense responsese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e4“indicating local induction of RNA silencing and salicylic acid (SA)-dependent defense responses”
Claim · model-written summary
SA pretreatment reduced lesion size and viral systemic spread in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- SA pretreatmente5
- Interactor
- ToRSVe0pathogen
- Outcome
- Lesion size and viral systemic spreade5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e5“Lesion size and viral systemic spread were reduced with SA pretreatment but enhanced in NahG transgenic lines deficient in SA accumulation”
Claim · model-written summary
Lesion size and viral systemic spread were enhanced in NahG transgenic tobacco lines deficient in SA accumulation.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- NahG transgenic lines deficient in SA accumulatione5
- Interactor
- ToRSVe0pathogen
- Outcome
- Lesion size and viral systemic spreade5
- Direction
- enhancede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e5“Lesion size and viral systemic spread were reduced with SA pretreatment but enhanced in NahG transgenic lines deficient in SA accumulation”
Claim · model-written summary
SA-dependent mechanisms are suggested to play a key role in limiting ToRSV spread in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- limiting ToRSV spreade6
- Qualifier
- SA-dependent mechanismse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e6“suggesting that SA-dependent mechanisms play a key role in limiting ToRSV spread in tobacco”
Claim · model-written summary
Restriction of virus infection was enhanced in tobacco lines expressing the P1-HC-Pro suppressor of silencing.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- transgenic lines expressing the P1-HC-Pro suppressor of silencinge7
- Gene
- P1-HC-Proe7
- Outcome
- Restriction of virus infectione7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e7“Restriction of virus infection was enhanced in transgenic lines expressing the P1-HC-Pro suppressor of silencing”
Claim · model-written summary
Knocking down SA-inducible RNA-dependent RNA polymerase 1 exacerbated the necrotic reaction in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Knocking down the SA-inducible RNA-dependent RNA polymerase 1e1
- Gene
- RNA-dependent RNA polymerase 1e1
- Outcome
- necrotic reactione1
- Direction
- exacerbatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e1“Knocking down the SA-inducible RNA-dependent RNA polymerase 1 exacerbated the necrotic reaction but did not affect viral systemic spread”
Claim · model-written summary
Knocking down SA-inducible RNA-dependent RNA polymerase 1 did not affect viral systemic spread in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Knocking down the SA-inducible RNA-dependent RNA polymerase 1e1
- Gene
- RNA-dependent RNA polymerase 1e1
- Outcome
- viral systemic spreade1
- Direction
- did not affecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e1“Knocking down the SA-inducible RNA-dependent RNA polymerase 1 exacerbated the necrotic reaction but did not affect viral systemic spread”
Claim · model-written summary
ToRSV-infected tobacco plants were susceptible to reinoculation by ToRSV or Tobacco mosaic virus.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- Tobacco mosaic virus
Tobacco mosaic viruse1pathogen - Second interactor
- ToRSVe1pathogen
- Outcome
- susceptible to reinoculatione1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ToRSV-infected tobacco plants were susceptible to reinoculation by ToRSV or Tobacco mosaic virus, although a small reduction in lesion size was observed”
Claim · model-written summary
A small reduction in lesion size was observed in ToRSV-infected tobacco after reinoculation.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- lesion sizee1
- Direction
- small reductione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“ToRSV-infected tobacco plants were susceptible to reinoculation by ToRSV or Tobacco mosaic virus, although a small reduction in lesion size was observed”
Claim · model-written summary
The moderate systemic resistance suggests inefficient induction or spread of RNA silencing and systemic acquired resistance signal molecules in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- moderate systemic resistancee1
- Qualifier
- inefficient induction or spread of RNA silencing and systemic acquired resistance signal moleculese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we show that systemic infection by ToRSV is restricted in tobacco”
e1“This moderate systemic resistance suggests inefficient induction or spread of RNA silencing and systemic acquired resistance signal molecules”
The same questions in other papers
salicylic acid · disease · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.