Abstract
Differentiation of adventitious buds and somatic embryos from mature zygotic embryos of Picea sitchensis (Bong.) Carr. is described. Adventitious buds were formed on embryos pulse-treated with 250 microM benzyladenine for 2 h and cultured on medium lacking growth regulators. Buds were initiated at different frequencies and sites depending on when the BA-pulsed embryos were transferred to fresh culture medium. Embryogenic callus was formed when the zygotic embryos were cultured on medium containing 10 microM 2,4-dichlorophenoxyacetic acid and 5 microM benzyladenine. Although 50% of the embryos gave rise to embryogenic callus, only 20% of the callus continued to proliferate after subculture. Proliferation of new somatic embryos occurred from both the embryonic region and the suspensor region on previously formed somatic embryos. The pattern for development of adventitious buds and somatic embryos in Picea sitchensis is compared to that in Picea abies under similar culture conditions.
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Claims 6
Claim · model-written summary
A benzyladenine pulse followed by regulator-free culture produced adventitious buds on Picea sitchensis embryos.
- Taxon
- Picea sitchensis
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Picea sitchensist0 - Organ / stage
- embryose0
- Treatment
- pulse-treated with 250 microM benzyladenine for 2 he0
- Outcome
- Adventitious budse0
- Direction
- formede0
- Qualifier
- cultured on medium lacking growth regulatorse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Adventitious buds were formed on embryos pulse-treated with 250 microM benzyladenine for 2 h and cultured on medium lacking growth regulators.”
t0“Picea sitchensis”
Claim · model-written summary
Bud initiation frequency and site varied with the timing of transfer to fresh medium.
- Taxon
- Picea sitchensisPicea sitchensist0
- Treatment
- BA-pulsed embryos were transferred to fresh culture mediume0
- Outcome
- different frequencies and sitese0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Buds were initiated at different frequencies and sites depending on when the BA-pulsed embryos were transferred to fresh culture medium.”
t0“Picea sitchensis”
Claim · model-written summary
Embryogenic callus formed from Picea sitchensis zygotic embryos on medium containing auxin and benzyladenine.
- Taxon
- Picea sitchensisPicea sitchensist0
- Organ / stage
- zygotic embryose0
- Treatment
- medium containing 10 microM 2,4-dichlorophenoxyacetic acid and 5 microM benzyladeninee0
- Outcome
- Embryogenic calluse0
- Direction
- formede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenic callus was formed when the zygotic embryos were cultured on medium containing 10 microM 2,4-dichlorophenoxyacetic acid and 5 microM benzyladenine.”
t0“Picea sitchensis”
Claim · model-written summary
Half of Picea sitchensis embryos yielded embryogenic callus.
- Taxon
- Picea sitchensisPicea sitchensist0
- Organ / stage
- embryose0
- Outcome
- gave rise to embryogenic calluse0
- Value
- 50%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“50% of the embryos gave rise to embryogenic callus”
t0“Picea sitchensis”
Claim · model-written summary
Only 20% of Picea sitchensis embryogenic callus continued proliferating after subculture.
- Taxon
- Picea sitchensisPicea sitchensist0
- Organ / stage
- calluse0
- Outcome
- continued to proliferatee0
- Value
- 20%e0
- Qualifier
- after subculturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only 20% of the callus continued to proliferate after subculture”
t0“Picea sitchensis”
Claim · model-written summary
New somatic embryos proliferated from both embryonic and suspensor regions of earlier somatic embryos.
- Taxon
- Picea sitchensisPicea sitchensist0
- Organ / stage
- embryonic region and the suspensor regione0
- Outcome
- Proliferation of new somatic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proliferation of new somatic embryos occurred from both the embryonic region and the suspensor region on previously formed somatic embryos.”
t0“Picea sitchensis”
The same questions in other papers
N-benzyladenine · growth · Picea sitchensis (Sitka spruce)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
in vitro · growth · Picea sitchensis (Sitka spruce)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.