Abstract
Bread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of a high cytosolic K(+) /Na(+) ratio in the leaves of salt stressed plants. The Kna1 locus encompasses a large DNA fragment, the distal 14% of chromosome 4DL. Limited recombination has been observed at this locus making it difficult to map genetically and identify the causal gene. Here, we decipher the function of TaHKT1;5-D, a candidate gene underlying the Kna1 locus. Transport studies using the heterologous expression systems Saccharomyces cerevisiae and Xenopus laevis oocytes indicated that TaHKT1;5-D is a Na(+) -selective transporter. Transient expression in Arabidopsis thaliana mesophyll protoplasts and in situ polymerase chain reaction indicated that TaHKT1;5-D is localised on the plasma membrane in the wheat root stele. RNA interference-induced silencing decreased the expression of TaHKT1;5-D in transgenic bread wheat lines which led to an increase in the Na(+) concentration in the leaves. This indicates that TaHKT1;5-D retrieves Na(+) from the xylem vessels in the root and has an important role in restricting the transport of Na(+) from the root to the leaves in bread wheat. Thus, TaHKT1;5-D confers the essential salinity tolerance mechanism in bread wheat associated with the Kna1 locus via shoot Na(+) exclusion and is critical in maintaining a high K(+) /Na(+) ratio in the leaves. These findings show there is potential to increase the salinity tolerance of bread wheat by manipulation of HKT1;5 genes.
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Claims 8
Claim · model-written summary
TaHKT1;5-D is a sodium-selective transporter.
- Taxon
- bread wheat
Triticum aestivumt0 - Gene
- TaHKT1;5-De0
- Outcome
- Na(+) -selective transportere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHKT1;5-D is a Na(+) -selective transporter”
t0“bread wheat”
Claim · model-written summary
TaHKT1;5-D localizes to the plasma membrane of the wheat root stele.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- root stelee0
- Gene
- TaHKT1;5-De0
- Outcome
- localised on the plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHKT1;5-D is localised on the plasma membrane in the wheat root stele”
Claim · model-written summary
RNA interference lowered TaHKT1;5-D expression in transgenic bread wheat.
- Taxon
- bread wheatTriticum aestivume0
- Treatment
- RNA interference-induced silencinge0
- Gene
- TaHKT1;5-De0
- Outcome
- expression of TaHKT1;5-De0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference-induced silencing decreased the expression of TaHKT1;5-D in transgenic bread wheat lines”
Claim · model-written summary
Silencing TaHKT1;5-D increased leaf sodium concentration.
- Taxon
- bread wheatTriticum aestivume0
- Treatment
- RNA interference-induced silencinge0
- Gene
- TaHKT1;5-De0
- Outcome
- Na(+) concentration in the leavese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference-induced silencing decreased the expression of TaHKT1;5-D in transgenic bread wheat lines which led to an increase in the Na(+) concentration in the leaves”
t0“bread wheat”
Claim · model-written summary
The authors infer TaHKT1;5-D retrieves sodium from root xylem.
- Taxon
- bread wheatTriticum aestivumt0
- Gene
- TaHKT1;5-De0
- Outcome
- retrieves Na(+) from the xylem vessels in the roote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHKT1;5-D retrieves Na(+) from the xylem vessels in the root”
t0“bread wheat”
Claim · model-written summary
The authors infer TaHKT1;5-D restricts sodium transport from roots to leaves.
- Taxon
- bread wheatTriticum aestivume0
- Gene
- TaHKT1;5-De1
- Outcome
- restricting the transport of Na(+) from the root to the leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“restricting the transport of Na(+) from the root to the leaves in bread wheat”
e1“This indicates that TaHKT1;5-D retrieves Na(+)”
Claim · model-written summary
The authors infer TaHKT1;5-D supports the high leaf potassium-to-sodium ratio associated with salinity tolerance.
- Taxon
- bread wheatTriticum aestivume0
- Gene
- TaHKT1;5-De0
- Outcome
- maintaining a high K(+) /Na(+) ratio in the leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaHKT1;5-D confers the essential salinity tolerance mechanism in bread wheat associated with the Kna1 locus via shoot Na(+) exclusion and is critical in maintaining a high K(+) /Na(+) ratio in the leaves”
Claim · model-written summary
The authors see potential to raise bread wheat salinity tolerance through HKT1;5 gene manipulation.
- Taxon
- bread wheatTriticum aestivume0
- Gene
- HKT1;5 genese0
- Outcome
- increase the salinity tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potential to increase the salinity tolerance of bread wheat by manipulation of HKT1;5 genes”
The same questions in other papers
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