Abstract
cDNA clones for two isogenes of nitrate reductase (NR) have been isolated from rapeseed (Brassica napus L.) androgenetic haploid embryos induced by microspore culture. NR mRNA accumulation can be detected by northern hybridization at 14 d after culture initiation when embryos develop to the heart/torpedo-shaped stage. Whole-mount in situ hybridization experiments demonstrate that the mRNA accumulation is developmental stage specific. In addition, even when cultured in media containing no nitrate, embryos accumulated NR mRNA to almost the same level as the control. This indicates the unique regulation of NR in embryogenesis in which NR mRNA transcription is activated in a developmental stage-specific manner that is independent of nitrate induction. In zygotic embryogenesis, a stage-specific accumulation of NR mRNA was also observed. By contrast, the obvious effect of nitrate on NR expression that has been reported in many plant species was also confirmed in rapeseed leaf. Quantitative combined reverse transcription-polymerase chain reaction analysis suggests that the flexible and variable regulation of NR expression, which is organ specific, nitrogen metabolite specific, and developmental stage specific, is caused principally by regulation of one major structural gene.
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Claims 8
Claim · model-written summary
Two nitrate reductase isogene cDNA clones were isolated from rapeseed androgenetic embryos.
Verbatim
e0“cDNA clones for two isogenes of nitrate reductase (NR) have been isolated from rapeseed (Brassica napus L.) androgenetic haploid embryos induced by microspore culture”
Claim · model-written summary
NR mRNA was detectable at 14 days after culture initiation at the heart or torpedo embryo stage.
Verbatim
e0“NR mRNA accumulation can be detected by northern hybridization at 14 d after culture initiation when embryos develop to the heart/torpedo-shaped stage”
e1“rapeseed (Brassica napus L.)”
Claim · model-written summary
NR mRNA accumulation in androgenetic embryos was developmental stage specific.
Verbatim
e0“the mRNA accumulation is developmental stage specific”
e1“rapeseed (Brassica napus L.)”
e2“NR mRNA accumulation”
Claim · model-written summary
Embryos cultured without nitrate accumulated NR mRNA at almost the control level.
Verbatim
e0“even when cultured in media containing no nitrate, embryos accumulated NR mRNA to almost the same level as the control”
e1“rapeseed (Brassica napus L.)”
Claim · model-written summary
NR transcription during embryogenesis was activated by developmental stage independent of nitrate induction.
Verbatim
e0“NR mRNA transcription is activated in a developmental stage-specific manner that is independent of nitrate induction”
e1“rapeseed (Brassica napus L.)”
Claim · model-written summary
NR mRNA also accumulated in a stage specific way in zygotic embryos.
Verbatim
e0“In zygotic embryogenesis, a stage-specific accumulation of NR mRNA was also observed”
e1“rapeseed (Brassica napus L.)”
Claim · model-written summary
Nitrate affected NR expression in rapeseed leaves.
Not reportedComparatorDirection
Verbatim
e0“the obvious effect of nitrate on NR expression that has been reported in many plant species was also confirmed in rapeseed leaf”
Claim · model-written summary
One major structural gene principally accounts for organ, nitrogen metabolite and developmental stage variation in NR expression.
Verbatim
e0“the flexible and variable regulation of NR expression, which is organ specific, nitrogen metabolite specific, and developmental stage specific, is caused principally by regulation of one major structural gene”
e1“rapeseed (Brassica napus L.)”
The same questions in other papers
nitrogen · physiology: enzyme activity · Brassica napus (oilseed rape)Positive and negative results
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.