Abstract
In Arabidopsis thaliana, Argonaute1 (AGO1) interacts with miR168 to modulate the small RNA regulatory pathway. However, the underlying mechanism of regulation and relationship between AGO1 and miR168 is poorly understood in the cash crop Solanum lycopersicum (tomato). We previously found that SlAGO1A and SlAGO1B were cleaved by miR168 in tomato. In this study, we show that SlAGO1A and SlAGO1B accumulate in miR168-sponge transgenic plants, and that expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit. Accumulation of miR156 was found when 4m-SlAGO1A was at an early developmental stage compared to the wild type and original SlAGO1A transgenic plants, and miR172 was highly expressed in adult 4m-SlAGO1A compared to the controls. In addition, the expression of multiple small RNAs was altered in 4m-SlAGO1A. Taken together, our data provide novel insights into the interaction between SlAGO1s and miR168 in determining growth rate, phase change, leaf epinasty, fruit initiation and expansion, and other developmental processes in tomato.
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Claims 8
Claim · model-written summary
SlAGO1A and SlAGO1B accumulate in miR168-sponge transgenic tomato plants.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- miR168-sponge transgenic plantse1
- Gene
- SlAGO1Ae1
- Outcome
- SlAGO1A and SlAGO1Be1
- Direction
- accumulatee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“miR168 influences phase transition, leaf epinasty, and fruit development via SlAGO1s in tomato”
e1“SlAGO1A and SlAGO1B accumulate in miR168-sponge transgenic plants”
Claim · model-written summary
Expression of miR168-resistant SlAGO1 in tomato causes defects in growth rate.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- expression of miR168-resistant SlAGO1A (4m-SlAGO1A)e2
- Outcome
- growth ratee2
- Direction
- defectse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit”
Claim · model-written summary
Expression of miR168-resistant SlAGO1 in tomato causes defects in floral timing.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- expression of miR168-resistant SlAGO1A (4m-SlAGO1A)e2
- Outcome
- floral timinge2
- Direction
- defectse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit”
Claim · model-written summary
Expression of miR168-resistant SlAGO1 in tomato causes defects in leaves.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- expression of miR168-resistant SlAGO1A (4m-SlAGO1A)e2
- Outcome
- leavese2
- Direction
- defectse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit”
Claim · model-written summary
Expression of miR168-resistant SlAGO1 in tomato causes defects in fruit.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- expression of miR168-resistant SlAGO1A (4m-SlAGO1A)e2
- Outcome
- fruite2
- Direction
- defectse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit”
Claim · model-written summary
miR156 accumulated at an early developmental stage in 4m-SlAGO1A tomato compared with wild type and original SlAGO1A transgenic plants.
- Taxon
- tomatoSolanum lycopersicume2
- Organ / stage
- early developmental stagee3
- Treatment
- 4m-SlAGO1Ae3
- Outcome
- miR156e3
- Direction
- Accumulatione3
- Comparator
- wild type and original SlAGO1A transgenic plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit”
e3“Accumulation of miR156 was found when 4m-SlAGO1A was at an early developmental stage compared to the wild type and original SlAGO1A transgenic plants”
Claim · model-written summary
miR172 was highly expressed in adult 4m-SlAGO1A tomato compared with controls.
- Taxon
- tomatoSolanum lycopersicume2
- Organ / stage
- adulte4
- Treatment
- 4m-SlAGO1Ae4
- Outcome
- miR172e4
- Direction
- highly expressede4
- Comparator
- controlse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit”
e4“miR172 was highly expressed in adult 4m-SlAGO1A compared to the controls”
Claim · model-written summary
The expression of multiple small RNAs was altered in 4m-SlAGO1A tomato.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- 4m-SlAGO1Ae5
- Outcome
- expression of multiple small RNAse5
- Direction
- alterede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit”
e5“the expression of multiple small RNAs was altered in 4m-SlAGO1A”
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