Abstract
Insect egg deposition activates plant defence, but very little is known about signalling events that control this response. In Arabidopsis thaliana, oviposition by Pieris brassicae triggers salicylic acid (SA) accumulation and induces the expression of defence genes. This is similar to the recognition of pathogen-associated molecular patterns (PAMPs), which are involved in PAMP-triggered immunity (PTI). Here, the involvement of known signalling components of PTI in response to oviposition was studied. Treatment with P. brassicae egg extract caused a rapid induction of early PAMP-responsive genes. In addition, expression of the defence gene PR-1 required EDS1, SID2, and, partially, NPR1, thus implicating the SA pathway downstream of egg recognition. PR-1 expression was triggered by a non-polar fraction of egg extract and by an oxidative burst modulated through the antagonistic action of EDS1 and NUDT7, but which did not depend on the NADPH oxidases RBOHD and RBOHF. Searching for receptors of egg-derived elicitors, a receptor-like kinase mutant, lecRK-I.8, was identified which shows a much reduced induction of PR-1 in response to egg extract treatment. These results demonstrate the importance of the SA pathway in response to egg-derived elicitor(s) and unravel intriguing similarities between the detection of insect eggs and PTI in Arabidopsis.
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Claims 5
Claim · model-written summary
Egg extract rapidly induced early PAMP-responsive genes.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- P. brassicae egg extracte0
- Interactor
- P. brassicae
_Pieris_brassicae_-_Foret_de_Bouconne%2C_France_-_female.jpg?width=60)
Pieris brassicaee0herbivore - Outcome
- early PAMP-responsive genese0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Pieris_brassicae_-_Foret_de_Bouconne%2C_France_-_female.jpg?width=500)
Verbatim
e0“Treatment with P. brassicae egg extract caused a rapid induction of early PAMP-responsive genes.”
t0“Arabidopsis thaliana”
Claim · model-written summary
PR-1 expression required EDS1, SID2 and partly NPR1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- EDS1, SID2, and, partially, NPR1e0
- Outcome
- expression of the defence gene PR-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the defence gene PR-1 required EDS1, SID2, and, partially, NPR1”
t0“Arabidopsis thaliana”
Claim · model-written summary
A non-polar egg extract fraction triggered PR-1 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- non-polar fraction of egg extracte0
- Outcome
- PR-1 expressione0
- Direction
- triggerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PR-1 expression was triggered by a non-polar fraction of egg extract”
t0“Arabidopsis thaliana”
Claim · model-written summary
An oxidative burst modulated by EDS1 and NUDT7 triggered PR-1 expression without dependence on RBOHD and RBOHF.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- EDS1 and NUDT7e0
- Outcome
- oxidative burste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by an oxidative burst modulated through the antagonistic action of EDS1 and NUDT7, but which did not depend on the NADPH oxidases RBOHD and RBOHF.”
t0“Arabidopsis thaliana”
Claim · model-written summary
lecRK-I.8 showed much reduced PR-1 induction after egg extract treatment.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- lecRK-I.8e0
- Treatment
- egg extract treatmente0
- Outcome
- induction of PR-1e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lecRK-I.8, was identified which shows a much reduced induction of PR-1 in response to egg extract treatment.”
t0“Arabidopsis thaliana”
The same questions in other papers
Pieris brassicae · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
1 in the opposite direction
- negative
botanical · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.