Abstract
This study was designed to quantitatively analyze the expression of thaumatin-like protein (TLP) at the transcriptional level in different sorghum lines when they were infested with greenbugs. Three sorghum lines, Tx7000, PI550607, and PI550610, were used. RNAs were isolated from the different sorghum lines that were infested with greenbugs at different infestation times. The resultant mRNA was reverse transcribed into cDNA, and the RT-PCR products were separated by agarose gel. Then, real-time PCR data of the TLP gene expression were analyzed in comparison with the beta-actin gene as a reference. The expression levels of the TLP gene were also compared between samples. The results showed that the transcripts of the TLP were induced by greenbug feeding and the increased levels were time-dependent. In the susceptible line, the TLP's transcripts increased several thousand-fold at 120 hours post infestation, while for the two resistant sorghum lines the TLP expression level increased less than one hundred-fold compared to the controls. This is the first demonstration that thaumatin-like proteins are involved in plant defense response against insects.
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Claims 3
Claim · model-written summary
Greenbug feeding induced TLP transcripts over time.
- Treatment
- greenbug feedinge0
- Interactor
- greenbug
Schizaphis graminume0pest - Gene
- TLPe0
- Outcome
- transcripts of the TLPe0
- Direction
- increasede0
SorghumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that the transcripts of the TLP were induced by greenbug feeding and the increased levels were time-dependent”
t0“Differential Expression of Thaumatin-Like Proteins in Sorghum Infested with Greenbugs”
Claim · model-written summary
TLP transcripts in the susceptible line increased several thousand-fold at 120 hours.
- Material
- susceptible linee0
- Treatment
- infestatione0
- Interactor
- GreenbugsSchizaphis graminumt0pest
- Gene
- TLPe0
- Outcome
- TLP's transcriptse0
- Direction
- increasede0
- Value
- several thousand-folde0
- Qualifier
- 120 hours post infestatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the susceptible line, the TLP's transcripts increased several thousand-fold at 120 hours post infestation”
t0“Differential Expression of Thaumatin-Like Proteins in Sorghum Infested with Greenbugs”
Claim · model-written summary
TLP expression in the two resistant lines rose less than one hundred-fold versus controls.
- Interactor
- GreenbugsSchizaphis graminumt0e1pest
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“for the two resistant sorghum lines the TLP expression level increased less than one hundred-fold compared to the controls”
t0“Differential Expression of Thaumatin-Like Proteins in Sorghum Infested with Greenbugs”
e1“The results showed that the transcripts of the TLP were induced by greenbug feeding and the increased levels were time-dependent”
The same questions in other papers
infection · quality: protein · SorghumPositive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.