Abstract
To assess the efficacy of near lethal heat stress for endodormancy release of Superior Seedless grapevine buds, single-node cuttings were soaked for 1 h in hot water (50 degrees C) and then forced for one month. The effects of hot water treatment (HWT) on budburst, metabolic changes of carbohydrates, proline, free polyamines PAs (putrescine (Put), spermidine (Spd) and spermine (Spm)) and antioxidant enzymes' activity were investigated in bud under forcing conditions. Near-lethal heat stress caused a transient increase in starch hydrolysis, leading to an abrupt accumulation of soluble sugars, especially sucrose concentration during the first 3 days after treatment. This accumulation of soluble sugars coincided with a brief stimulation of the antioxidant system represented by ascorbate peroxidase (APX; EC 1.11.1.11), peroxidase (POD; EC 1.11.1.7) as well as an increase in the concentration of proline and free polyamines, especially putrescine (Put) and spermidine (Spd). These changes, which occurred immediately upon treatment, appear to be related with a process leading to endodormancy release. These results support the argument that a temporary and acute oxidative stress is involved in the mechanism leading to dormancy release and budbreak. Furthermore, it is possible that the stimulation of both peroxidases' activity and proline biosynthesis activated the pentose phosphate pathway (PPP) which helped the bud to overcome endodormancy. After this initial period and towards budbreak initiation, there was a rapid decline in the concentration of soluble sugars, proline and Put, while, Spm and Spd became abundant. Such post stress changes appear to be associated with the reactivation of growth leading to an earlier and more vigorous budbreak. The metabolic response to HWT was compared to those observed after bud chilling or the application of restbreaking agents such as hydrogen cyanamide (HC). The similarity in the response to these various stimuli suggests the presence of common regulatory pathways involved in bud dormancy release and subsequent sprouting.
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Claims 11
Claim · model-written summary
Heat stress transiently increased starch hydrolysis.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)
Vitis viniferat0 - Treatment
- heat stresse0
- Outcome
- starch hydrolysise0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Near-lethal heat stress caused a transient increase in starch hydrolysis”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
Heat stress abruptly accumulated soluble sugars, particularly sucrose, in the first three days.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Treatment
- heat stresse1
- Outcome
- soluble sugarse0
- Direction
- accumulatione0
- Qualifier
- first 3 days after treatmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leading to an abrupt accumulation of soluble sugars, especially sucrose concentration during the first 3 days after treatment.”
e1“Near-lethal heat stress”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
The antioxidant enzymes APX and POD were briefly stimulated during sugar accumulation.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Treatment
- heat stresse1
- Gene
- ascorbate peroxidase (APX; EC 1.11.1.11), peroxidase (POD; EC 1.11.1.7)e0
- Outcome
- antioxidant systeme0
- Direction
- stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This accumulation of soluble sugars coincided with a brief stimulation of the antioxidant system represented by ascorbate peroxidase (APX; EC 1.11.1.11), peroxidase (POD; EC 1.11.1.7)”
e1“Near-lethal heat stress”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
Proline and free polyamines, especially putrescine and spermidine, increased after treatment.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Treatment
- heat stresse1
- Outcome
- concentration of proline and free polyaminese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as well as an increase in the concentration of proline and free polyamines, especially putrescine (Put) and spermidine (Spd).”
e1“Near-lethal heat stress”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
Immediate metabolic changes appeared related to endodormancy release.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Treatment
- treatmente0
- Outcome
- endodormancy releasee0
- Qualifier
- immediately upon treatmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These changes, which occurred immediately upon treatment, appear to be related with a process leading to endodormancy release.”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
The authors interpret temporary acute oxidative stress as involved in dormancy release and budbreak.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Outcome
- dormancy release and budbreake0
- Qualifier
- temporary and acute oxidative stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results support the argument that a temporary and acute oxidative stress is involved in the mechanism leading to dormancy release and budbreak.”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
The authors suggest peroxidase activity and proline biosynthesis may activate the pentose phosphate pathway and help buds overcome endodormancy.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Outcome
- overcome endodormancye0
- Qualifier
- pentose phosphate pathway (PPP)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is possible that the stimulation of both peroxidases' activity and proline biosynthesis activated the pentose phosphate pathway (PPP) which helped the bud to overcome endodormancy.”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
Soluble sugars, proline and Put declined rapidly toward budbreak.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Treatment
- heat stresse1
- Outcome
- concentration of soluble sugars, proline and Pute0
- Direction
- declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was a rapid decline in the concentration of soluble sugars, proline and Put”
e1“Near-lethal heat stress”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
Spm and Spd became abundant toward budbreak.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Treatment
- heat stresse1
- Outcome
- Spm and Spde0
- Direction
- abundante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Spm and Spd became abundant.”
e1“Near-lethal heat stress”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
Post-stress metabolic changes appeared associated with earlier, more vigorous budbreak.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Organ / stage
- budbreake0
- Outcome
- earlier and more vigorous budbreake0
- Direction
- earlier and more vigorouse0
- Qualifier
- post stress changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such post stress changes appear to be associated with the reactivation of growth leading to an earlier and more vigorous budbreak.”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
Claim · model-written summary
Similarity among heat, chilling and restbreaking responses suggested common regulatory pathways.
- Taxon
- Superior Seedless grapevine cv. (Vitis vinifera L.)Vitis viniferat0
- Outcome
- common regulatory pathways involved in bud dormancy release and subsequent sproutinge0
- Qualifier
- similarity in the response to these various stimulie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The similarity in the response to these various stimuli suggests the presence of common regulatory pathways involved in bud dormancy release and subsequent sprouting.”
t0“Superior Seedless grapevine cv. (Vitis vinifera L.)”
The same questions in other papers
heat · quality: sugar starch · Vitis vinifera (wine grape)Positive and negative results
2 in the opposite direction
- no effect
- no effect
heat · molecular: metabolites · Vitis vinifera (wine grape)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.