Paper · 1968 · Journal of Experimental Botany

Effects of Monochromatic Radiations on Growth of Pelargonium Callus Tissue

H. BAILEY WARD; B. DWAIN VANCE

9
claims
17
verbatim quotes
18
citations
2
research areas
—
Not open access

Abstract

Pith callus tissues were grown under continuous blue (450 mμ), green (545 mμ), red (650 mμ), and ‘white’ (full-spectrum) light, and in the dark for 22 days at 27±2° C at energy levels of 15,000 ergs cm−2 sec−1. Mean increases in fresh weight of tissues grown under ‘white’ and blue light were significantly greater than those of tissues grown in green and red light and in the dark. Tissues grown in the dark yielded mean fresh weight increases significantly lower than tissues grown under blue, red, and ‘white’ light. No significant differences were shown between blue and ‘white’, red and green, and green and dark treatments respectively. Cell differentiation occurred in all treatments only to the extent of vessel element formation. There were no differences in degree of differentiation between treatments. It was proposed that the high-energy reaction of photomorphogenesis was in operation in the Pelargonium callus tissue. The results indicated the presence in the tissue of high-energy photoreceptor(s). The use of high-intensity, incandescent illumination for experimental procedures approximating natural conditions of irradiation was indicated as desirable for pith callus tissues of Pelargonium zonale var. Enchantress Fiat.

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Claims 9

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

White and blue light produced greater callus fresh-weight gains than green, red or dark treatments.

Taxon
PelargoniumPelargoniumt0
Treatment
‘white’ and blue lighte0
Outcome
increases in fresh weighte0
Direction
greatere0
Comparator
green and red light and in the darke0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium
Pelargonium · Taxon · picture of the genus (one of its species)Krzysztof Golik · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Mean increases in fresh weight of tissues grown under ‘white’ and blue light were significantly greater than those of tissues grown in green and red light and in the dark”

✓ located verbatim in the source · abstract, characters 211–381 · show in text ↑
t0

“Pelargonium Callus Tissue”

✓ located verbatim in the source · title, characters 49–74 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Dark-grown callus had lower fresh-weight gains than blue, red and white light treatments.

Taxon
PelargoniumPelargoniumt0
Treatment
in the darke0
Outcome
fresh weight increasese0
Direction
lowere0
Comparator
blue, red, and ‘white’ lighte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium
Pelargonium · Taxon · picture of the genus (one of its species)Krzysztof Golik · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Tissues grown in the dark yielded mean fresh weight increases significantly lower than tissues grown under blue, red, and ‘white’ light”

✓ located verbatim in the source · abstract, characters 383–518 · show in text ↑
t0

“Pelargonium Callus Tissue”

✓ located verbatim in the source · title, characters 49–74 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Fresh-weight gain did not differ significantly between blue and white light.

Taxon
PelargoniumPelargoniumt0
Treatment
bluee0
Outcome
significant differencese0
Direction
No significant differencese0
Comparator
‘white’e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium
Pelargonium · Taxon · picture of the genus (one of its species)Krzysztof Golik · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“No significant differences were shown between blue and ‘white’, red and green, and green and dark treatments respectively”

✓ located verbatim in the source · abstract, characters 520–641 · show in text ↑
t0

“Pelargonium Callus Tissue”

✓ located verbatim in the source · title, characters 49–74 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Fresh-weight gain did not differ significantly between red and green light.

Taxon
PelargoniumPelargoniumt0
Treatment
rede0
Outcome
significant differencese0
Direction
No significant differencese0
Comparator
greene0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium
Pelargonium · Taxon · picture of the genus (one of its species)Krzysztof Golik · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“No significant differences were shown between blue and ‘white’, red and green, and green and dark treatments respectively”

✓ located verbatim in the source · abstract, characters 520–641 · show in text ↑
t0

“Pelargonium Callus Tissue”

✓ located verbatim in the source · title, characters 49–74 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Fresh-weight gain did not differ significantly between green light and darkness.

Taxon
PelargoniumPelargoniumt0
Treatment
greene0
Outcome
significant differencese0
Direction
No significant differencese0
Comparator
darke0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium
Pelargonium · Taxon · picture of the genus (one of its species)Krzysztof Golik · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“No significant differences were shown between blue and ‘white’, red and green, and green and dark treatments respectively”

✓ located verbatim in the source · abstract, characters 520–641 · show in text ↑
t0

“Pelargonium Callus Tissue”

✓ located verbatim in the source · title, characters 49–74 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

All light treatments produced vessel elements as the only observed differentiation.

Taxon
PelargoniumPelargoniumt0
Organ / stage
Celle0
Outcome
vessel element formatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium
Pelargonium · Taxon · picture of the genus (one of its species)Krzysztof Golik · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Cell differentiation occurred in all treatments only to the extent of vessel element formation”

✓ located verbatim in the source · abstract, characters 643–737 · show in text ↑
t0

“Pelargonium Callus Tissue”

✓ located verbatim in the source · title, characters 49–74 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Degree of callus differentiation did not differ among treatments.

Taxon
PelargoniumPelargoniumt0
Treatment
treatmentse0
Outcome
degree of differentiatione0
Direction
no differencese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium
Pelargonium · Taxon · picture of the genus (one of its species)Krzysztof Golik · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“There were no differences in degree of differentiation between treatments”

✓ located verbatim in the source · abstract, characters 739–812 · show in text ↑
t0

“Pelargonium Callus Tissue”

✓ located verbatim in the source · title, characters 49–74 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors inferred high-energy photoreceptors in Pelargonium callus.

Taxon
PelargoniumPelargoniumt0
Outcome
presence in the tissue of high-energy photoreceptor(s)e0
Qualifier
high-energy photoreceptor(s)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium
Pelargonium · Taxon · picture of the genus (one of its species)Krzysztof Golik · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The results indicated the presence in the tissue of high-energy photoreceptor(s)”

✓ located verbatim in the source · abstract, characters 933–1013 · show in text ↑
t0

“Pelargonium Callus Tissue”

✓ located verbatim in the source · title, characters 49–74 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend high-intensity incandescent illumination for experiments approximating natural irradiation conditions in Pelargonium pith callus.

Taxon
Pelargonium zonale var. Enchantress FiatPelargonium zonalee0
Outcome
use of high-intensity, incandescent illumination for experimental procedures approximating natural conditions of irradiatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pelargonium zonale
Pelargonium zonale · TaxonChrisaliv · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The use of high-intensity, incandescent illumination for experimental procedures approximating natural conditions of irradiation was indicated as desirable for pith callus tissues of Pelargonium zonale var. Enchantress Fiat”

✓ located verbatim in the source · abstract, characters 1015–1238 · show in text ↑

light · growth · PelargoniumPositive and negative results

5 papers: 4 positive, 1 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.