Paper · 1986 · European Journal of Biochemistry

Inhibition of carotenoid accumulation and abscisic acid biosynthesis in fluridone‐treated dark‐grown barley

Patricia E. GAMBLE; John E. MULLET

11
claims
22
verbatim quotes
127
citations
2
research areas
✓
Open access

Abstract

Treatment of dark-grown barley with 0.1 mM fluridone inhibited carotenoid accumulation but did not alter plastid biogenesis. Plastids isolated from dark-grown control and dark-grown fluridone-treated plants were similar in size and protein compositions. Dehydration of dark-grown control barley caused abscisic acid levels to increase 30-40-fold in 4 h, while plants treated with 0.1 mM fluridone accumulated very little abscisic acid in response to dehydration. These results suggest that fluridone-treated plants do not accumulate abscisic acid because of carotenoid deficiency rather than plastid dysfunction. Dark-grown barley plants treated with 0.31 microM fluridone accumulated low levels of carotenoids. Dehydration of these plants resulted in a 4-8-fold increase in abscisic acid and a decrease in antheraxanthin, violaxanthin and neoxanthin, but no change in beta-carotene or lutein plus zeaxanthin levels. This result is consistent with the suggestion that xanthophylls are precursors to abscisic acid in dehydrated plants.

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Claims 11

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Fluridone at 0.1 mM inhibited carotenoid accumulation in dark-grown barley relative to the dark-grown control.

Treatment
0.1 mM fluridonee1
Outcome
carotenoid accumulatione1
Direction
inhibitede1
Comparator
dark-grown controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Treatment of dark-grown barley with 0.1 mM fluridone inhibited carotenoid accumulation but did not alter plastid biogenesis”

✓ located verbatim in the source · abstract, characters 0–123 · show in text ↑
e2

“Plastids isolated from dark-grown control and dark-grown fluridone-treated plants were similar in size and protein compositions”

✓ located verbatim in the source · abstract, characters 125–252 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Fluridone at 0.1 mM did not alter plastid biogenesis in dark-grown barley.

Treatment
0.1 mM fluridonee1
Outcome
plastid biogenesise1
Direction
did not altere1
Comparator
dark-grown controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Treatment of dark-grown barley with 0.1 mM fluridone inhibited carotenoid accumulation but did not alter plastid biogenesis”

✓ located verbatim in the source · abstract, characters 0–123 · show in text ↑
e2

“Plastids isolated from dark-grown control and dark-grown fluridone-treated plants were similar in size and protein compositions”

✓ located verbatim in the source · abstract, characters 125–252 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Plastids from fluridone-treated and control barley were similar in size and protein composition.

Treatment
dark-grown fluridone-treated plantse2
Outcome
size and protein compositionse2
Direction
similare2
Comparator
dark-grown controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Treatment of dark-grown barley with 0.1 mM fluridone inhibited carotenoid accumulation but did not alter plastid biogenesis”

✓ located verbatim in the source · abstract, characters 0–123 · show in text ↑
e2

“Plastids isolated from dark-grown control and dark-grown fluridone-treated plants were similar in size and protein compositions”

✓ located verbatim in the source · abstract, characters 125–252 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Dehydration of control barley increased abscisic acid levels 30-40-fold within 4 h.

Treatment
Dehydratione3
Outcome
abscisic acid levelse3
Direction
increasee3
Value
30-40-folde3
Qualifier
in 4 he3
Comparator
dark-grown control barleye3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e3

“Dehydration of dark-grown control barley caused abscisic acid levels to increase 30-40-fold in 4 h”

✓ located verbatim in the source · abstract, characters 254–352 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Fluridone at 0.1 mM caused barley to accumulate very little abscisic acid in response to dehydration.

Treatment
0.1 mM fluridonee4
Outcome
abscisic acide4
Direction
very littlee4
Qualifier
in response to dehydratione4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Treatment of dark-grown barley with 0.1 mM fluridone inhibited carotenoid accumulation but did not alter plastid biogenesis”

✓ located verbatim in the source · abstract, characters 0–123 · show in text ↑
e4

“while plants treated with 0.1 mM fluridone accumulated very little abscisic acid in response to dehydration”

✓ located verbatim in the source · abstract, characters 354–461 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Dark-grown barley treated with 0.31 microM fluridone accumulated low levels of carotenoids.

Treatment
0.31 microM fluridonee5
Outcome
carotenoidse5
Direction
lowe5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“Dark-grown barley plants treated with 0.31 microM fluridone accumulated low levels of carotenoids”

✓ located verbatim in the source · abstract, characters 613–710 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Dehydration of barley treated with 0.31 microM fluridone increased abscisic acid 4-8-fold.

Treatment
Dehydratione6
Outcome
abscisic acide6
Direction
increasee6
Value
4-8-folde6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“Dark-grown barley plants treated with 0.31 microM fluridone accumulated low levels of carotenoids”

✓ located verbatim in the source · abstract, characters 613–710 · show in text ↑
e6

“Dehydration of these plants resulted in a 4-8-fold increase in abscisic acid”

✓ located verbatim in the source · abstract, characters 712–788 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Dehydration decreased antheraxanthin, violaxanthin and neoxanthin in barley treated with 0.31 microM fluridone.

Treatment
Dehydratione6
Outcome
antheraxanthin, violaxanthin and neoxanthine7
Direction
decreasee7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“Dark-grown barley plants treated with 0.31 microM fluridone accumulated low levels of carotenoids”

✓ located verbatim in the source · abstract, characters 613–710 · show in text ↑
e6

“Dehydration of these plants resulted in a 4-8-fold increase in abscisic acid”

✓ located verbatim in the source · abstract, characters 712–788 · show in text ↑
e7

“a decrease in antheraxanthin, violaxanthin and neoxanthin, but no change in beta-carotene or lutein plus zeaxanthin levels”

✓ located verbatim in the source · abstract, characters 793–915 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Dehydration did not change beta-carotene or lutein plus zeaxanthin levels in barley treated with 0.31 microM fluridone.

Treatment
Dehydratione6
Outcome
beta-carotene or lutein plus zeaxanthin levelse7
Direction
no changee7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e5

“Dark-grown barley plants treated with 0.31 microM fluridone accumulated low levels of carotenoids”

✓ located verbatim in the source · abstract, characters 613–710 · show in text ↑
e6

“Dehydration of these plants resulted in a 4-8-fold increase in abscisic acid”

✓ located verbatim in the source · abstract, characters 712–788 · show in text ↑
e7

“a decrease in antheraxanthin, violaxanthin and neoxanthin, but no change in beta-carotene or lutein plus zeaxanthin levels”

✓ located verbatim in the source · abstract, characters 793–915 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Xanthophylls are suggested to be precursors of abscisic acid in dehydrated barley.

Compound
xanthophyllse2
Outcome
abscisic acide2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Treatment of dark-grown barley with 0.1 mM fluridone inhibited carotenoid accumulation but did not alter plastid biogenesis”

✓ located verbatim in the source · abstract, characters 0–123 · show in text ↑
e2

“This result is consistent with the suggestion that xanthophylls are precursors to abscisic acid in dehydrated plants”

✓ located verbatim in the source · abstract, characters 917–1033 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Fluridone-treated barley does not accumulate abscisic acid because of carotenoid deficiency rather than plastid dysfunction.

Treatment
fluridone-treated plantse2
Outcome
accumulate abscisic acide2
Qualifier
carotenoid deficiency rather than plastid dysfunctione2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Treatment of dark-grown barley with 0.1 mM fluridone inhibited carotenoid accumulation but did not alter plastid biogenesis”

✓ located verbatim in the source · abstract, characters 0–123 · show in text ↑
e2

“These results suggest that fluridone-treated plants do not accumulate abscisic acid because of carotenoid deficiency rather than plastid dysfunction”

✓ located verbatim in the source · abstract, characters 463–611 · show in text ↑

light · quality: protein · Hordeum vulgare (barley)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

3 in the opposite direction

  1. positive
  2. positive
  3. positive
See all 4 papers →

drought · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results

32 papers: 12 positive, 20 negative or no effect

19 papers in the same direction as this paper · 12 in the opposite direction

  1. positive
  2. positive
  3. positive

28 papers more in this group

See all 32 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.