Abstract
The vacuolar membrane (tonoplast) of plant cells contains a polytopic integral membrane protein with six membrane-spanning domains and cytoplasmically oriented amino-terminal and carboxy-terminal domains. This protein, tonoplast intrinsic protein (TIP), is a member of the membrane intrinsic protein (MIP) family of proteins, a family of channel proteins found in a variety of organisms. In bean seeds, alpha-TIP is synthesized on the rough endoplasmic reticulum and its transport to the tonoplast is mediated by the secretory system. In this study, we report that a polypeptide segment that includes the sixth membrane domain and the cytoplasmic tail of 18 amino acids of alpha-TIP is sufficient to target the reporter protein phosphinotricine acetyltransferase to the tonoplast of stably transformed tobacco cells. To determine if the carboxy-terminal cytoplasmic tail of alpha-TIP contains important tonoplast targeting information, a deletion construct lacking the 15 carboxy-terminal amino acids was introduced for transient expression in tobacco cells; we found that the slightly truncated protein still accumulated in the tonoplast. From these results, we concluded that a transmembrane domain of a tonoplast protein probably contains sufficient information for transport to the tonoplast. Whether such transport occurs by bulk flow or involves specific cellular machinery remains to be determined.
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Claims 2
Claim · model-written summary
The alpha-TIP sixth membrane domain and cytoplasmic tail targeted a reporter protein to the tonoplast of transformed tobacco cells.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- a polypeptide segment that includes the sixth membrane domain and the cytoplasmic tail of 18 amino acids of alpha-TIPe0
- Gene
- alpha-TIPe0
- Outcome
- target the reporter protein phosphinotricine acetyltransferase to the tonoplaste0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a polypeptide segment that includes the sixth membrane domain and the cytoplasmic tail of 18 amino acids of alpha-TIP is sufficient to target the reporter protein phosphinotricine acetyltransferase to the tonoplast of stably transformed tobacco cells”
Claim · model-written summary
An alpha-TIP construct lacking 15 carboxy-terminal amino acids still accumulated in the tobacco tonoplast.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- a deletion construct lacking the 15 carboxy-terminal amino acidse0
- Outcome
- slightly truncated protein still accumulated in the tonoplaste0
Not reportedGeneComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a deletion construct lacking the 15 carboxy-terminal amino acids was introduced for transient expression in tobacco cells; we found that the slightly truncated protein still accumulated in the tonoplast”
The same questions in other papers
loss of function · quality: protein · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.