Abstract
ABSTRACT Polypeptide synthesis and accumulation were examined in the roots of tomato seedlings exposed to a polyethylene glycol‐imposed water deficit stress. In these roots, the synthesis of a number of polypeptides was induced, while that of several others was enhanced or repressed. To examine the role played by abscisic acid (ABA) in co‐ordinating the accumulation of these proteins, water‐deficit‐stress‐responsive polypeptide synthesis was investigated in the roots of the ABA‐deficient mutant flacca. In the roots of this mutant, the ability to accumulate a complete set of water‐deficit‐stress‐responsive polypeptides was impaired, indicating that ABA is required for their synthesis. The role of ABA was further examined by exposing the roots of both genotypes to exogenous ABA, which, with one exception, elicited the accumulation of all water‐deficit‐stress‐responsive proteins. Polyethylene glycol‐induced polypeptide accumulation was accompanied by a 1·6‐fold increase in the level of endogenous ABA in the roots of wild‐type plants and a 5‐fold increase in the roots of flc . Thus, although the absolute level was lower than that of the wild‐type, flc has the capacity to accumulate ABA in its roots. When fluridone was used to prevent the biosynthesis of ABA, the accumulation of several water‐deficit‐stress‐responsive polypeptides was reduced further. The synthesis of polypeptides was also examined in the roots of salt‐treated seedlings. Salt altered the accumulation of several polypeptides, all of which were previously observed in water‐deficit‐stressed roots, indicating that their synthesis was the result of the osmotic component of the salt stress. However, the accumulation of these polypeptides was not impaired in flc roots, indicating that the role played by ABA in regulating their accumulation in salt‐and polyethylene glycol‐treated roots differs. As such, salt‐ and water‐deficit‐stress‐induced changes in gene expression may be effected by different mechanisms, at least at the level of polypeptide accumulation.
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Claims 8
Claim · model-written summary
Water deficit induced, enhanced, or repressed different polypeptides in roots.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- polyethylene glycol‐imposed water deficit stresse1
- Outcome
- synthesis of a number of polypeptidese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In these roots, the synthesis of a number of polypeptides was induced, while that of several others was enhanced or repressed.”
t0“tomato”
t1“tomato”
e1“a polyethylene glycol‐imposed water deficit stress”
Claim · model-written summary
The flacca mutant could not accumulate the full set of water deficit responsive polypeptides.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- flaccae1
- Organ / stage
- rootse0
- Outcome
- ability to accumulate a complete set of water‐deficit‐stress‐responsive polypeptidese0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the roots of this mutant, the ability to accumulate a complete set of water‐deficit‐stress‐responsive polypeptides was impaired”
t0“tomato”
t1“tomato”
e1“ABA‐deficient mutant flacca”
Claim · model-written summary
Exogenous ABA elicited nearly all water deficit responsive proteins in both genotypes.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- exogenous ABAe0
- Outcome
- accumulation of all water‐deficit‐stress‐responsive proteinse0
- Direction
- elicitede0
- Qualifier
- with one exceptione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous ABA, which, with one exception, elicited the accumulation of all water‐deficit‐stress‐responsive proteins”
t0“tomato”
Claim · model-written summary
PEG increased endogenous root ABA 1·6 fold in wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- wild‐type plantse0
- Treatment
- Polyethylene glycole0
- Outcome
- level of endogenous ABAe0
- Direction
- increasee0
- Value
- 1·6‐folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Polyethylene glycol‐induced polypeptide accumulation was accompanied by a 1·6‐fold increase in the level of endogenous ABA in the roots of wild‐type plants”
t0“tomato”
Claim · model-written summary
PEG increased root ABA five fold in flc.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- flce0
- Treatment
- Polyethylene glycole1
- Outcome
- endogenous ABAe1
- Direction
- increasee0
- Value
- 5‐folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 5‐fold increase in the roots of flc”
t0“tomato”
t1“tomato”
e1“Polyethylene glycol‐induced polypeptide accumulation was accompanied by a 1·6‐fold increase in the level of endogenous ABA in the roots of wild‐type plants”
Claim · model-written summary
Fluridone further reduced accumulation of several responsive polypeptides.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- fluridonee0
- Outcome
- accumulation of several water‐deficit‐stress‐responsive polypeptidese0
- Direction
- reduced furthere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When fluridone was used to prevent the biosynthesis of ABA, the accumulation of several water‐deficit‐stress‐responsive polypeptides was reduced further.”
t0“tomato”
Claim · model-written summary
Salt altered accumulation of polypeptides also seen under water deficit.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Salte0
- Outcome
- accumulation of several polypeptidese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salt altered the accumulation of several polypeptides, all of which were previously observed in water‐deficit‐stressed roots”
t0“tomato”
Claim · model-written summary
Salt responsive polypeptide accumulation was unimpaired in flc roots.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- flce0
- Organ / stage
- rootse0
- Outcome
- accumulation of these polypeptidese0
- Direction
- not impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of these polypeptides was not impaired in flc roots”
t0“tomato”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results
9 papers in the same direction as this paper · 5 in the opposite direction
- positive
- negative
- no effect
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.