Paper · 2004 · Journal of Experimental Botany

A physical, enzymatic, and genetic characterization of perturbations in the seeds of the brownseed tomato mutants

A. B. Downie

17
claims
33
verbatim quotes
18
citations
3
research areas
✓
Open access

Abstract

The brownseed mutants (bs(1), bs(2), and bs(4)) of tomato all possess dark testae and deleteriously affect seed germination speed and/or final percentage. Poor germination performance of the bs(1) but not the bs(4) mutant, was due to greater impediment to radicle egress. Testa toughening (bs(1)) was prevented by drying in N(2). However, poor germination speed was hardly affected by drying. GA(4+7) did not ameliorate germination percentage or speed (bs(1), bs(2)), whereas bs(4) seeds commenced radicle protrusion sooner and had a greater germination percentage. bs(1) mutant seeds have two times more catalase activity while those of bs(4) contained six times more peroxidase and almost two times more catalase activity than WTs. bs(4) release only half of the reactive oxygen species into the media than WT during imbibition. EPR detected the presence of free radicals in bs(1) and its WT. bs mutants were epistatic to 12 anthocyaninless mutations, at least some of which produce seeds of lighter than usual testa colour. Macro-arrays of subtractive, suppressive PCR products identified differentially regulated transcripts between seeds of bs(4) and WT. EST identity suggests bs(4) does not exit the developmental programme upon attaining maturity.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 17

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Tomato brownseed mutants possess dark testae and impair germination speed or final percentage.

Material
brownseed mutantse0
Outcome
dark testae and deleteriously affect seed germination speed and/or final percentagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The brownseed mutants (bs(1), bs(2), and bs(4)) of tomato all possess dark testae and deleteriously affect seed germination speed and/or final percentage.”

✓ located verbatim in the source · abstract, characters 0–154 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Greater impediment to radicle egress explains poor germination in bs(1), but not bs(4).

Material
bs(1)e0
Outcome
Poor germination performancee0
Qualifier
greater impediment to radicle egresse0
Comparator
bs(4)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Poor germination performance of the bs(1) but not the bs(4) mutant, was due to greater impediment to radicle egress.”

✓ located verbatim in the source · abstract, characters 155–271 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Drying in nitrogen prevented bs(1) testa toughening.

Material
bs(1)e0
Treatment
drying in N(2)e0
Outcome
Testa tougheninge0
Direction
preventede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Testa toughening (bs(1)) was prevented by drying in N(2).”

✓ located verbatim in the source · abstract, characters 272–329 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Drying hardly affected poor germination speed.

Treatment
dryinge0
Outcome
poor germination speede0
Direction
hardly affectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“However, poor germination speed was hardly affected by drying.”

✓ located verbatim in the source · abstract, characters 330–392 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

GA(4+7) did not ameliorate bs(1) germination percentage or speed.

Material
bs(1)e0
Treatment
GA(4+7)e0
Outcome
germination percentage or speede0
Direction
did not amelioratee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“GA(4+7) did not ameliorate germination percentage or speed (bs(1), bs(2))”

✓ located verbatim in the source · abstract, characters 393–466 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

GA(4+7) did not ameliorate bs(2) germination percentage or speed.

Material
bs(2)e0
Treatment
GA(4+7)e0
Outcome
germination percentage or speede0
Direction
did not amelioratee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“GA(4+7) did not ameliorate germination percentage or speed (bs(1), bs(2))”

✓ located verbatim in the source · abstract, characters 393–466 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under GA(4+7), bs(4) seeds commenced radicle protrusion sooner.

Material
bs(4)e0
Treatment
GA(4+7)e0
Outcome
radicle protrusione0
Direction
soonere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“GA(4+7) did not ameliorate germination percentage or speed (bs(1), bs(2)), whereas bs(4) seeds commenced radicle protrusion sooner and had a greater germination percentage.”

✓ located verbatim in the source · abstract, characters 393–565 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under GA(4+7), bs(4) seeds had greater germination percentage.

Material
bs(4)e0
Treatment
GA(4+7)e0
Outcome
germination percentagee0
Direction
greatere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“GA(4+7) did not ameliorate germination percentage or speed (bs(1), bs(2)), whereas bs(4) seeds commenced radicle protrusion sooner and had a greater germination percentage.”

✓ located verbatim in the source · abstract, characters 393–565 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

bs(1) seeds have twice the catalase activity of wild types.

Material
bs(1)e0
Outcome
catalase activitye0
Direction
moree0
Value
two timese0
Comparator
WTse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bs(1) mutant seeds have two times more catalase activity while those of bs(4) contained six times more peroxidase and almost two times more catalase activity than WTs.”

✓ located verbatim in the source · abstract, characters 566–733 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

bs(4) seeds contain six times more peroxidase than wild types.

Material
bs(4)e0
Outcome
peroxidasee0
Direction
moree0
Value
six timese0
Comparator
WTse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bs(1) mutant seeds have two times more catalase activity while those of bs(4) contained six times more peroxidase and almost two times more catalase activity than WTs.”

✓ located verbatim in the source · abstract, characters 566–733 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

bs(4) seeds contain almost twice the catalase activity of wild types.

Material
bs(4)e0
Outcome
catalase activitye0
Direction
moree0
Value
almost two timese0
Comparator
WTse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bs(1) mutant seeds have two times more catalase activity while those of bs(4) contained six times more peroxidase and almost two times more catalase activity than WTs.”

✓ located verbatim in the source · abstract, characters 566–733 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

During imbibition bs(4) releases half the reactive oxygen species released by WT.

Material
bs(4)e0
Organ / stage
imbibitione0
Outcome
reactive oxygen speciese0
Direction
lowerinferrede0
Value
halfe0
Comparator
WTe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bs(4) release only half of the reactive oxygen species into the media than WT during imbibition.”

✓ located verbatim in the source · abstract, characters 734–830 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Free radicals were detected in bs(1).

Material
bs(1)e0
Outcome
presence of free radicalse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“EPR detected the presence of free radicals in bs(1) and its WT.”

✓ located verbatim in the source · abstract, characters 831–894 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Free radicals were detected in the bs(1) wild type.

Material
WTe0
Outcome
presence of free radicalse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“EPR detected the presence of free radicals in bs(1) and its WT.”

✓ located verbatim in the source · abstract, characters 831–894 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Brownseed mutants were epistatic to 12 anthocyaninless mutations.

Material
bs mutantse0
Outcome
epistatice0
Value
12e0
Qualifier
anthocyaninless mutationse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“bs mutants were epistatic to 12 anthocyaninless mutations”

✓ located verbatim in the source · abstract, characters 895–952 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Differentially regulated transcripts were identified between bs(4) and WT seeds.

Material
bs(4)e0
Outcome
differentially regulated transcriptse0
Comparator
WTe0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Macro-arrays of subtractive, suppressive PCR products identified differentially regulated transcripts between seeds of bs(4) and WT.”

✓ located verbatim in the source · abstract, characters 1027–1159 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

EST identity suggests bs(4) remains in the developmental programme at maturity.

Material
bs(4)e0
Organ / stage
maturitye0
Outcome
does not exit the developmental programmee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“EST identity suggests bs(4) does not exit the developmental programme upon attaining maturity.”

✓ located verbatim in the source · abstract, characters 1160–1254 · show in text ↑
t0

“tomato”

✓ located verbatim in the source · title, characters 99–105 · show in text ↑

Gibberellic acid · growth · Solanum lycopersicum (tomato)Results differ

87 papers: 80 positive, 7 negative or no effect

79 papers in the same direction as this paper · 7 in the opposite direction

  1. positive
  2. negative
  3. no effect

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processing · growth · Solanum lycopersicum (tomato)Positive and negative results

66 papers: 45 positive, 21 negative or no effect

20 papers in the same direction as this paper · 45 in the opposite direction

  1. positive
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  3. positive

62 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.