Abstract
We isolated a calcineurin B-like protein (CBL)-interacting protein kinase (EC 2.7.11.1) homolog (VuCIPK1) from cowpea (Vigna unguiculata (L.) Walp.) with significant similarity to AtCIPK3 (86% amino acid identity), which is involved in K+ transport. VuCIPK1 mRNA was detected in the whole plant by RT-PCR. Any significant change in VuCIPK1 mRNA levels was not detected when cowpea was subjected to various environmental stresses. Immunoblot assay with an anti-VuCIPK1 specific antibody and an anti-CBL antibody showed that immunologically CIPK- and CBL-related polypeptides were preferentially associated with membrane fractions of cowpea. The anti-VuCIPK1 antibody cross-reacted to two different CIPK-related polypeptides with relative molecular masses of 51 kDa in root and 55 kDa in mature leaf. Immunoprecipitation assay by anti-VuCIPK1 antibody using cowpea leaf extracts showed that endogenous VuCIPK1 was autophosphorylated on the threonine residues in response to salt stress. The recombinant VuCIPK1 phosphorylated casein in the presence of Mn2+. These observations suggest that membrane-associated VuCIPK1 is involved osmotic stress-activated kinase regulated by its phosphorylation status.
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Claims 9
Claim · model-written summary
Cowpea VuCIPK1 homolog has 86% amino acid identity to AtCIPK3.
- Taxon
- cowpea
Vigna unguiculatae0 - Gene
- VuCIPK1e0
- Outcome
- amino acid identitye0
- Value
- 86%e0
- Comparator
- AtCIPK3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“homolog (VuCIPK1) from cowpea (Vigna unguiculata (L.) Walp.) with significant similarity to AtCIPK3 (86% amino acid identity)”
Claim · model-written summary
VuCIPK1 mRNA was detected throughout the cowpea plant.
- Taxon
- cowpeaVigna unguiculatat0
- Organ / stage
- whole plante0
- Gene
- VuCIPK1e0
- Outcome
- VuCIPK1 mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VuCIPK1 mRNA was detected in the whole plant by RT-PCR”
t0“cowpea”
Claim · model-written summary
Environmental stresses produced no significant change in cowpea VuCIPK1 mRNA levels.
- Taxon
- cowpeaVigna unguiculatae0
- Treatment
- various environmental stressese0
- Gene
- VuCIPK1e0
- Outcome
- VuCIPK1 mRNA levelse0
- Direction
- no_changeinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Any significant change in VuCIPK1 mRNA levels was not detected when cowpea was subjected to various environmental stresses”
Claim · model-written summary
CIPK- and CBL-related polypeptides were preferentially associated with cowpea membrane fractions.
- Taxon
- cowpeaVigna unguiculatae0
- Gene
- CIPK- and CBL-related polypeptidese0
- Outcome
- preferentially associated with membrane fractionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CIPK- and CBL-related polypeptides were preferentially associated with membrane fractions of cowpea”
Claim · model-written summary
The antibody cross-reacted with a 51 kDa CIPK-related polypeptide in cowpea root.
- Taxon
- cowpeaVigna unguiculatat0
- Organ / stage
- roote0
- Gene
- CIPK-related polypeptidese0
- Outcome
- relative molecular massese0
- Value
- 51 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two different CIPK-related polypeptides with relative molecular masses of 51 kDa in root and 55 kDa in mature leaf”
t0“cowpea”
Claim · model-written summary
The antibody cross-reacted with a 55 kDa CIPK-related polypeptide in cowpea mature leaf.
- Taxon
- cowpeaVigna unguiculatat0
- Organ / stage
- mature leafe0
- Gene
- CIPK-related polypeptidese0
- Outcome
- relative molecular massese0
- Value
- 55 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two different CIPK-related polypeptides with relative molecular masses of 51 kDa in root and 55 kDa in mature leaf”
t0“cowpea”
Claim · model-written summary
Endogenous VuCIPK1 autophosphorylated on threonine residues in response to salt stress.
- Taxon
- cowpeaVigna unguiculatat0
- Treatment
- salt stresse0
- Gene
- VuCIPK1e0
- Outcome
- autophosphorylated on the threonine residuese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous VuCIPK1 was autophosphorylated on the threonine residues in response to salt stress”
t0“cowpea”
Claim · model-written summary
Recombinant VuCIPK1 phosphorylated casein in the presence of Mn2+.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- VuCIPK1e0
- Compound
- Mn2+e0
- Outcome
- phosphorylated caseine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recombinant VuCIPK1 phosphorylated casein in the presence of Mn2+”
t0“cowpea”
Claim · model-written summary
The authors suggest membrane-associated VuCIPK1 acts as an osmotic stress activated kinase regulated by phosphorylation status.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- VuCIPK1e0
- Outcome
- osmotic stress-activated kinasee0
- Qualifier
- regulated by its phosphorylation statuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“membrane-associated VuCIPK1 is involved osmotic stress-activated kinase regulated by its phosphorylation status”
t0“cowpea”
The same questions in other papers
climate site · molecular: expression · Vigna unguiculata (cowpea)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.