Abstract
Traditional methods used to study strawberry ripening-related gene function are time-consuming, and require at least 15 months from initiating the transformation experiment until the first ripe fruits are available for analysis. To accelerate data acquisition during gene function studies, we explored a transient assay method that employs an Agrobacterium-mediated RNAi (AmRNAi) technique in post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar. Our results showed that artificial white light induced strong expression of Fa′chalcone synthase (Fa′CHS), Fa′chalcone isomerase (Fa′CHI), and Fa′flavonoid 3′-hydroxylase orthologues (Fa′F3′H) in post-harvest fruit. Fa′CHS and Fa′F3′H function was subsequently examined by performing AmRNAi with post-harvest fruit. Although reduction of light-induced Fa′F3′H expression by AmRNAi resulted in no significant change in anthocyanin content, reduction of Fa′CHS significantly decreased anthocyanin levels, and up-regulated Fa′F3′H levels. Our results are consistent with previous data indicating that while CHS is required for anthocyanin accumulation during late stage strawberry fruit maturation, Fa′F3′H is not required. The novel system described here enabled gene function data to be available within 10 days of initiating the incubation period following infiltration. Therefore, we conclude our system is a valuable tool to elucidate the molecular mechanisms underlying light-induced ripening of strawberry fruit.
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Claims 5
Claim · model-written summary
Artificial white light strongly induced expression of Fa′CHS, Fa′CHI and Fa′F3′H in post-harvest strawberry fruit.
- Taxon
- Fragaria×ananassae0
- Material
- Sachinokae0
- Organ / stage
- post-harvest fruite1
- Treatment
- artificial white lighte1
- Gene
- Fa′chalcone synthase (Fa′CHS), Fa′chalcone isomerase (Fa′CHI), and Fa′flavonoid 3′-hydroxylase orthologues (Fa′F3′H)e1
- Outcome
- expressione1
- Direction
- inducede1
Not reportedComparator
Verbatim
e0“post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar”
e1“artificial white light induced strong expression of Fa′chalcone synthase (Fa′CHS), Fa′chalcone isomerase (Fa′CHI), and Fa′flavonoid 3′-hydroxylase orthologues (Fa′F3′H) in post-harvest fruit”
Claim · model-written summary
Silencing light-induced Fa′F3′H by AmRNAi did not significantly change anthocyanin content.
- Taxon
- Fragaria×ananassae0
- Treatment
- reduction of light-induced Fa′F3′H expression by AmRNAie2
- Gene
- Fa′F3′He2
- Outcome
- anthocyanin contente2
- Direction
- no significant changee2
Not reportedComparator
Verbatim
e0“post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar”
e2“reduction of light-induced Fa′F3′H expression by AmRNAi resulted in no significant change in anthocyanin content”
Claim · model-written summary
Silencing Fa′CHS by AmRNAi significantly decreased anthocyanin levels.
- Taxon
- Fragaria×ananassae0
- Treatment
- reduction of Fa′CHSe3
- Gene
- Fa′CHSe3
- Outcome
- anthocyanin levelse3
- Direction
- significantly decreasede3
Not reportedComparator
Verbatim
e0“post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar”
e3“reduction of Fa′CHS significantly decreased anthocyanin levels, and up-regulated Fa′F3′H levels”
Claim · model-written summary
Silencing Fa′CHS by AmRNAi up-regulated Fa′F3′H levels.
- Taxon
- Fragaria×ananassae0
- Treatment
- reduction of Fa′CHSe3
- Gene
- Fa′F3′He3
- Outcome
- Fa′F3′H levelse3
- Direction
- up-regulatede3
Not reportedComparator
Verbatim
e0“post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar”
e3“reduction of Fa′CHS significantly decreased anthocyanin levels, and up-regulated Fa′F3′H levels”
Claim · model-written summary
The AmRNAi system made strawberry gene function data available within 10 days, whereas traditional methods required at least 15 months.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae5 - Outcome
- gene function data to be availablee4
- Value
- within 10 dayse4
- Comparator
- Traditional methodse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e4“The novel system described here enabled gene function data to be available within 10 days of initiating the incubation period following infiltration”
e5“Traditional methods used to study strawberry ripening-related gene function are time-consuming, and require at least 15 months from initiating the transformation experiment until the first ripe fruits are available for analysis”
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light · quality: phytochemicals · Fragaria x ananassa (strawberry)Results differ
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.