Abstract
The role of auxin in controlling leaf expansion remains unclear. Experimental increases to normal auxin levels in expanding leaves have shown conflicting results, with both increases and decreases in leaf growth having been measured. Therefore, the effects of both auxin application and adjustment of endogenous leaf auxin levels on midrib elongation and final leaf size (fresh weight and area) were examined in attached primary monofoliate leaves of the common bean (Phaseolus vulgaris) and in early Arabidopsis rosette leaves. Aqueous auxin application inhibited long-term leaf blade elongation. Bean leaves, initially 40 to 50 mm in length, treated once with alpha-naphthalene acetic acid (1.0 mm), were, after 6 d, approximately 80% the length and weight of controls. When applied at 1.0 and 0.1 mm, alpha-naphthalene acetic acid significantly inhibited long-term leaf growth. The weak auxin, beta-naphthalene acetic acid, was effective at 1.0 mm; and a weak acid control, benzoic acid, was ineffective. Indole-3-acetic acid (1 microm, 10 microm, 0.1 mm, and 1 mm) required daily application to be effective at any concentration. Application of the auxin transport inhibitor, 1-N-naphthylphthalamic acid (1% [w/w] in lanolin), to petioles also inhibited long-term leaf growth. This treatment also was found to lead to a sustained elevation of leaf free indole-3-acetic acid content relative to untreated control leaves. Auxin-induced inhibition of leaf growth appeared not to be mediated by auxin-induced ethylene synthesis because growth inhibition was not rescued by inhibition of ethylene synthesis. Also, petiole treatment of Arabidopsis with 1-N-naphthylphthalamic acid similarly inhibited leaf growth of both wild-type plants and ethylene-insensitive ein4 mutants.
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Claims 10
Claim · model-written summary
Aqueous auxin inhibited long-term leaf blade elongation.
- Taxon
- Bean and Arabidopsist0
- Treatment
- Aqueous auxin applicatione0
- Outcome
- leaf blade elongatione0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Aqueous auxin application inhibited long-term leaf blade elongation”
t0“Bean and Arabidopsis”
Claim · model-written summary
Bean leaves given alpha-naphthalene acetic acid at 1.0 mm were about 80% of control length and weight after six days.
Verbatim
e0“Bean leaves, initially 40 to 50 mm in length, treated once with alpha-naphthalene acetic acid (1.0 mm), were, after 6 d, approximately 80% the length and weight of controls”
Claim · model-written summary
Alpha-naphthalene acetic acid at 1.0 and 0.1 mm significantly inhibited long-term leaf growth.
- Taxon
- Beant0
- Treatment
- alpha-naphthalene acetic acide0
- Outcome
- long-term leaf growthe0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“When applied at 1.0 and 0.1 mm, alpha-naphthalene acetic acid significantly inhibited long-term leaf growth”
t0“Bean”
Claim · model-written summary
Beta-naphthalene acetic acid was effective at 1.0 mm.
Not reportedComparator
Verbatim
e0“The weak auxin, beta-naphthalene acetic acid, was effective at 1.0 mm”
t0“Bean”
Claim · model-written summary
Benzoic acid control was ineffective.
Not reportedComparator
Verbatim
e0“a weak acid control, benzoic acid, was ineffective”
t0“Bean”
Claim · model-written summary
Indole-3-acetic acid required daily application to inhibit growth at any tested concentration.
- Taxon
- Beant0
- Treatment
- Indole-3-acetic acid (1 microm, 10 microm, 0.1 mm, and 1 mm)e0
- Outcome
- effectivee0
- Direction
- effectivee0
- Qualifier
- daily applicatione0
Not reportedComparator
Verbatim
e0“Indole-3-acetic acid (1 microm, 10 microm, 0.1 mm, and 1 mm) required daily application to be effective at any concentration”
t0“Bean”
Claim · model-written summary
Petiole NPA application inhibited long-term leaf growth.
- Taxon
- Beant0
- Treatment
- 1-N-naphthylphthalamic acid (1% [w/w] in lanolin)e0
- Outcome
- long-term leaf growthe0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Application of the auxin transport inhibitor, 1-N-naphthylphthalamic acid (1% [w/w] in lanolin), to petioles also inhibited long-term leaf growth”
t0“Bean”
Claim · model-written summary
Petiole NPA treatment sustained higher leaf free IAA than untreated controls.
Verbatim
e0“This treatment also was found to lead to a sustained elevation of leaf free indole-3-acetic acid content relative to untreated control leaves”
e1“1-N-naphthylphthalamic acid (1% [w/w] in lanolin)”
t0“Bean”
Claim · model-written summary
Inhibiting ethylene synthesis did not rescue auxin-induced leaf growth inhibition.
- Taxon
- Beant0
- Treatment
- inhibition of ethylene synthesise0
- Outcome
- growth inhibitione0
- Direction
- not rescuede0
Not reportedComparator
Verbatim
e0“growth inhibition was not rescued by inhibition of ethylene synthesis”
t0“Bean”
Claim · model-written summary
Petiole NPA inhibited leaf growth in wild-type Arabidopsis and ethylene-insensitive ein4 mutants.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- wild-type plants and ethylene-insensitive ein4 mutantse0
- Treatment
- 1-N-naphthylphthalamic acide0
- Outcome
- leaf growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“petiole treatment of Arabidopsis with 1-N-naphthylphthalamic acid similarly inhibited leaf growth of both wild-type plants and ethylene-insensitive ein4 mutants”
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See all 70 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.