Abstract
Survival of Botrytis cinerea conidia was studied after storage without pretreatments at different temperatures (-80 degrees C, -20 degrees C, 4 degrees C and 21 degrees C). Germination tests performed during 3 years showed that viability at 21 degrees C was completely lost after 1 month. Conidia stored for 30 months at -80 degrees C, -20 degrees C and 4 degrees C were able to germinate, respectively, at 79%, 8% and 0.2%. Changes in adenylate level, energy charge and respiration (O(2) consumption) made on each set of conidia were correlated to the germination rate. The 30-month-old stored conidia showed differences in pathogenicity tests on apples. While the pathogenic aggressiveness of conidia stored at -80 degrees C was almost the same as for fresh conidia, it decreased with increasing temperature of storage. An ultrastructural study made on conidia stored for 30 months at -80 degrees C has shown the emergence of a new wall layer in a retraction zone of the cytoplasm by comparison to fresh conidia. However, the integrity of the cytoplasmic content was maintained. The effects of low temperature storage, maintenance of cell integrity and pathogenicity of conidia of B. cinerea are discussed.
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Claims 2
Claim · model-written summary
After 30 months, conidia stored at −80 °C had almost the same pathogenic aggressiveness on apples as fresh conidia.
- Taxon
- apples
Malus domesticae0 - Treatment
- stored at -80 degrees Ce0
- Interactor
- Botrytis cinerea
Botrytis cinereae1 - Outcome
- pathogenic aggressivenesse0
- Direction
- almost the samee0
- Qualifier
- 30-month-olde0
- Comparator
- fresh conidiae0
Not reportedInteractor role
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 30-month-old stored conidia showed differences in pathogenicity tests on apples. While the pathogenic aggressiveness of conidia stored at -80 degrees C was almost the same as for fresh conidia”
e1“Survival of Botrytis cinerea conidia was studied”
Claim · model-written summary
Botrytis conidial pathogenic aggressiveness on apples decreased with increasing storage temperature.
- Taxon
- applesMalus domesticae0
- Treatment
- increasing temperature of storagee0
- Interactor
- Botrytis cinereaBotrytis cinereae1
- Outcome
- pathogenic aggressivenesse0
- Direction
- decreasede0
Not reportedInteractor role
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 30-month-old stored conidia showed differences in pathogenicity tests on apples. While the pathogenic aggressiveness of conidia stored at -80 degrees C was almost the same as for fresh conidia, it decreased with increasing temperature of storage”
e1“Survival of Botrytis cinerea conidia was studied”
The same questions in other papers
storage · Botrytis cinerea · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 1 reporting no difference from an alternative
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.