Abstract
OsTZF1 is a member of the CCCH-type zinc finger gene family in rice (Oryza sativa). Expression of OsTZF1 was induced by drought, high-salt stress, and hydrogen peroxide. OsTZF1 gene expression was also induced by abscisic acid, methyl jasmonate, and salicylic acid. Histochemical activity of β-glucuronidase in transgenic rice plants containing the promoter of OsTZF1 fused with β-glucuronidase was observed in callus, coleoptile, young leaf, and panicle tissues. Upon stress, OsTZF1-green fluorescent protein localization was observed in the cytoplasm and cytoplasmic foci. Transgenic rice plants overexpressing OsTZF1 driven by a maize (Zea mays) ubiquitin promoter (Ubi:OsTZF1-OX [for overexpression]) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence. RNA interference (RNAi) knocked-down plants (OsTZF1-RNAi) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls. Ubi:OsTZF1-OX plants showed improved tolerance to high-salt and drought stresses and vice versa for OsTZF1-RNAi plants. Microarray analysis revealed that genes related to stress, reactive oxygen species homeostasis, and metal homeostasis were regulated in the Ubi:OsTZF1-OX plants. RNA-binding assays indicated that OsTZF1 binds to U-rich regions in the 3' untranslated region of messenger RNAs, suggesting that OsTZF1 might be associated with RNA metabolism of stress-responsive genes. OsTZF1 may serve as a useful biotechnological tool for the improvement of stress tolerance in various plants through the control of RNA metabolism of stress-responsive genes.
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Claims 15
Claim · model-written summary
Drought, salt stress and hydrogen peroxide induced OsTZF1 expression.
- Taxon
- Rice
Oryza sativat0 - Treatment
- drought, high-salt stress, and hydrogen peroxidee0
- Gene
- OsTZF1e0
- Outcome
- Expression of OsTZF1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsTZF1 was induced by drought, high-salt stress, and hydrogen peroxide.”
t0“Rice”
Claim · model-written summary
Abscisic acid, methyl jasmonate and salicylic acid induced OsTZF1 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- abscisic acid, methyl jasmonate, and salicylic acide0
- Gene
- OsTZF1e0
- Outcome
- OsTZF1 gene expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsTZF1 gene expression was also induced by abscisic acid, methyl jasmonate, and salicylic acid.”
t0“Rice”
Claim · model-written summary
OsTZF1 promoter activity occurred in callus, coleoptile, young leaf and panicle tissues.
- Taxon
- riceOryza sativae0
- Organ / stage
- callus, coleoptile, young leaf, and panicle tissuese0
- Gene
- OsTZF1e0
- Outcome
- Histochemical activity of β-glucuronidasee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histochemical activity of β-glucuronidase in transgenic rice plants containing the promoter of OsTZF1 fused with β-glucuronidase was observed in callus, coleoptile, young leaf, and panicle tissues.”
Claim · model-written summary
OsTZF1 protein localized to cytoplasm and cytoplasmic foci under stress.
- Taxon
- RiceOryza sativat0
- Gene
- OsTZF1-green fluorescent proteine0
- Outcome
- localizatione0
- Qualifier
- Upon stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon stress, OsTZF1-green fluorescent protein localization was observed in the cytoplasm and cytoplasmic foci.”
t0“Rice”
Claim · model-written summary
OsTZF1 overexpression delayed germination.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressing OsTZF1e0
- Gene
- OsTZF1e0
- Outcome
- seed germinatione0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice plants overexpressing OsTZF1 driven by a maize (Zea mays) ubiquitin promoter (Ubi:OsTZF1-OX [for overexpression]) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence.”
Claim · model-written summary
OsTZF1 overexpression retarded seedling growth.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedling stagee0
- Treatment
- overexpressing OsTZF1e0
- Gene
- OsTZF1e0
- Outcome
- growthe0
- Direction
- retardatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice plants overexpressing OsTZF1 driven by a maize (Zea mays) ubiquitin promoter (Ubi:OsTZF1-OX [for overexpression]) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence.”
Claim · model-written summary
OsTZF1 overexpression delayed leaf senescence.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressing OsTZF1e0
- Gene
- OsTZF1e0
- Outcome
- leaf senescencee0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice plants overexpressing OsTZF1 driven by a maize (Zea mays) ubiquitin promoter (Ubi:OsTZF1-OX [for overexpression]) exhibited delayed seed germination, growth retardation at the seedling stage, and delayed leaf senescence.”
Claim · model-written summary
OsTZF1 knockdown caused early germination.
- Taxon
- RiceOryza sativat0
- Treatment
- OsTZF1-RNAie0
- Outcome
- seed germinatione0
- Direction
- earlye0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference (RNAi) knocked-down plants (OsTZF1-RNAi) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls.”
t0“Rice”
Claim · model-written summary
OsTZF1 knockdown enhanced seedling growth.
- Taxon
- RiceOryza sativat0
- Treatment
- OsTZF1-RNAie0
- Outcome
- seedling growthe0
- Direction
- enhancede0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference (RNAi) knocked-down plants (OsTZF1-RNAi) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls.”
t0“Rice”
Claim · model-written summary
OsTZF1 knockdown advanced leaf senescence.
- Taxon
- RiceOryza sativat0
- Treatment
- OsTZF1-RNAie0
- Outcome
- leaf senescencee0
- Direction
- earlye0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA interference (RNAi) knocked-down plants (OsTZF1-RNAi) showed early seed germination, enhanced seedling growth, and early leaf senescence compared with controls.”
t0“Rice”
Claim · model-written summary
OsTZF1 overexpression improved salt and drought tolerance.
- Taxon
- RiceOryza sativat0
- Treatment
- Ubi:OsTZF1-OXe0
- Outcome
- tolerance to high-salt and drought stressese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ubi:OsTZF1-OX plants showed improved tolerance to high-salt and drought stresses and vice versa for OsTZF1-RNAi plants.”
t0“Rice”
Claim · model-written summary
OsTZF1 knockdown reduced salt and drought tolerance.
- Taxon
- RiceOryza sativat0
- Treatment
- OsTZF1-RNAie0
- Outcome
- tolerance to high-salt and drought stressese0
- Direction
- vice versae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ubi:OsTZF1-OX plants showed improved tolerance to high-salt and drought stresses and vice versa for OsTZF1-RNAi plants.”
t0“Rice”
Claim · model-written summary
Overexpression regulated genes related to stress, reactive oxygen species and metal homeostasis.
- Taxon
- RiceOryza sativat0
- Material
- Ubi:OsTZF1-OXe0
- Gene
- genes related to stress, reactive oxygen species homeostasis, and metal homeostasise0
- Outcome
- genes related to stress, reactive oxygen species homeostasis, and metal homeostasis were regulatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Microarray analysis revealed that genes related to stress, reactive oxygen species homeostasis, and metal homeostasis were regulated in the Ubi:OsTZF1-OX plants.”
t0“Rice”
Claim · model-written summary
OsTZF1 bound U-rich regions in messenger RNA 3-prime untranslated regions.
- Taxon
- RiceOryza sativat0
- Gene
- OsTZF1e0
- Outcome
- binds to U-rich regions in the 3' untranslated region of messenger RNAse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-binding assays indicated that OsTZF1 binds to U-rich regions in the 3' untranslated region of messenger RNAs”
t0“Rice”
Claim · model-written summary
Authors suggest OsTZF1 as a tool for improving plant stress tolerance.
- Taxon
- plantsViridiplantaee0
- Gene
- OsTZF1e0
- Outcome
- improvement of stress tolerancee0
Not reportedDirection
Verbatim
e0“OsTZF1 may serve as a useful biotechnological tool for the improvement of stress tolerance in various plants through the control of RNA metabolism of stress-responsive genes.”
The same questions in other papers
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- no effect
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See all 80 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.